IL-10 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-10 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑10 (R&D Systems, Catalog # 417-ML)
  Ser19-Ser178
  Accession # NP_034678
• Specificity: Detects mouse IL-10 in ELISAs and Western blots. In sandwich immunoassays, less than 10% cross-reactivity with recombinant rat IL‑10 is observed, 1% cross‑reactivity with recombinant cotton rat IL-10 is observed, and less than 0.07% cross‑reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 sR, rhIL‑10 sR, recombinant canine IL-10, recombinant feline IL-10, and recombinant porcine IL-10 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL10
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse IL-10 Antibody (Catalog # MAB417)
  ELISA Detection: Mouse IL-10 Biotinylated Antibody (Catalog # BAF417)
  Standard: Recombinant Mouse IL-10 (Catalog # 417-ML)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody [Biotin]

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL-20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2 - 5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70% - 77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8 - 10). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha , or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12 - 14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15 - 17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
7. Hsu, D.-H. et al. (1990) Science 250:830.
8. Moore, K. et al. (1990) Science 248:1230.
9. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
10. Syto, R. et al. (1998) Biochemistry 37:16943.
11. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
12. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
13. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
14. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
15. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
16. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
17. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10 Antibody (BAF417)(19)

Publications using BAF417

• Gallant, RM;Snyder, JM;Ayres, JS; Fluoxetine promotes immunometabolic defenses to mediate host-pathogen cooperation during sepsis bioRxiv : the preprint server for biology 2023-11-18 [PMID: 38013994] (ELISA Detection, Mouse)
• K Kasahara, N Sasaki, HZ Amin, T Tanaka, S Horibe, T Yamashita, KI Hirata, Y Rikitake Depletion of Foxp3+ regulatory T cells augments CD4+ T cell immune responses in atherosclerosis-prone hypercholesterolemic mice Heliyon, 2022-07-19;8(7):e09981. 2022-07-19 [PMID: 35898604] (ELISA Detection, Mouse)
• Lim SW, Shin YJ, Cui S et al. Therapeutic effect of multiple functional minicircle vector encoding anti-CD25/IL-10/CXCR3 in allograft rejection model The Korean journal of internal medicine 6/21/2022 [PMID: 35725307]
• W Dawicki, C Li, J Town, X Zhang, JR Gordon Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells J. Allergy Clin. Immunol., 2016-10-26;139(5):1608-1620.e3. 2016-10-26 [PMID: 28277274] (ELISA Development (Detection), Mouse)
• AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova A critical role for IRF5 in regulating allergic airway inflammation Mucosal Immunol, 2016-10-19;10(3):716-726. 2016-10-19 [PMID: 27759022] (ELISA Development (Detection), Mouse)
• Jung-Yeon Lim Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis Sci Rep, 2016-06-01;6(0):26851. 2016-06-01 [PMID: 27246365] (ELISA Development (Detection), Mouse)
• Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice. J Immunol, 2014-11-24;194(1):334-48. 2014-11-24 [PMID: 25422508] (ELISA Development, Mouse)
• Michael A. Kron, Ahmed Metwali, Sanja Vodanovic-Jankovic, David Elliott Nematode Asparaginyl-tRNA Synthetase Resolves Intestinal Inflammation in Mice with T-Cell Transfer Colitis Clinical and Vaccine Immunology 2013-02-01 [PMID: 23254300]
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• M. Nedim Ince, David E. Elliott, Tommy Setiawan, Ahmed Metwali, Arthur Blum, Hung-lin Chen et al. Role of T cell TGF-beta signaling in intestinal cytokine responses and helminthic immune modulation European Journal of Immunology 2010-06-02 [PMID: 19544487]
• Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Microbes Infect., 2009-03-13;11(4):524-30. 2009-03-13 [PMID: 19286469] (ELISA Development, Mouse)
• Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology, 2008-04-03;125(2):272-9. 2008-04-03 [PMID: 18393969] (ELISA Development, Mouse)
• Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Tumor-specific Th17-polarized cells eradicate large established melanoma. Blood, 2008-03-19;112(2):362-73. 2008-03-19 [PMID: 18354038] (ELISA Development, Mouse)
• Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Infect. Immun., 2006-09-18;74(12):6730-8. 2006-09-18 [PMID: 16982824] (ELISA Development, Mouse)
• Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006-08-01;177(3):1967-74. 2006-08-01 [PMID: 16849510] (ELISA Development, Mouse)
• Wen H, Hogaboam CM, Gauldie J, Kunkel SL Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Am. J. Pathol., 2006-06-01;168(6):1940-50. 2006-06-01 [PMID: 16723709] (ELISA Development, Mouse)
• Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005-07-01;302(1):172-81. 2005-07-01 [PMID: 16023134] (ELISA Development, Mouse)
• Ellaban E, Bolgos G, Remick D Selective macrophage suppression during sepsis. Cell. Immunol., 2005-02-26;231(1):103-11. 2005-02-26 [PMID: 15919375] (ELISA Development, Mouse)
• Bednarski JJ, Warner RE, Rao T Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine. Arthritis Rheum., 2003-03-01;48(3):757-66. 2003-03-01 [PMID: 12632430] (ELISpot Development, Mouse)

Bioinformatics

• Gene Symbol: IL10
• Uniprot:
  • NP_034678()