IL-10 Antibody [Biotin]

• Reactivity: Eq
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-10 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant equine IL-10
  Ser19-Asn178
  Accession # Q28374
• Specificity: Detects equine IL-10 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant canine IL-10 and recombinant porcine IL-10, and less than 0.4% cross-reactivity with recombinant human IL-10, recombinant mouse IL-10, recombinant rat IL-10, and recombinant feline IL-10 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL10
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture:Equine IL-10 Antibody (Catalog # AF1605)
  ELISA Detection: Equine IL-10 Biotinylated Antibody (Catalog # BAF1605)
  Standard: Recombinant Equine IL-10 (Catalog # 1605-IL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody [Biotin]

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of mouse T helper 2 (Th2) cells that inhibited the cytokine production by Th1 cells. It is a pleiotropic cytokine that regulates the immune and inflammatory responses of hematopoietic cells (1, 2). IL-10 has immunosuppressive activities and has been shown to inhibit the effector functions of monocyte/macrophage and CD4⁺ T cells. Conversely, IL-10 has immunostimulatory activities and can induce the proliferation and cytotoxic activity of CD8⁺ T cells and NK cells. IL-10 also regulates the growth and differentiation of B cells, mast cells, dendritic cells and neutrophils (1). The biological activities of IL-10 is mediated by the heteromeric IL-10 receptor complex, which is composed of the ligand-binding IL-10R alpha and the accessory IL-10R beta subunits. Both subunits belong to the class II cytokine receptor family. IL-10R beta is also utilized as a subunit in the heterodimer receptor complex for IL-22, IL-28 and IL-29. Besides IL-10, five novel cytokines (IL-19, -20, -22, -24, and -26) that share structural and limited sequence homology with IL-10 have been identified. These proteins constitute the IL-10 cytokine family (3).

Equine IL-10 cDNA encodes a 178 amino acid residue (aa) precursor protein with an 18 aa signal peptide and 160 aa mature protein that contains two potential N-linked glycosylation sites. Analogous to human IL-10, equine IL-10 likely exists as nondisulfide-linked homodimers. Equine IL-10 shares 71% and 78% aa sequence homology with mouse and human IL-10, respectively.

1. Moore, K. et al. (2001) Annu. Rev. Immunol. 19:683.
2. Mocellin, S. et al. (2003) Trends in Immunol. 23:36.
3. Conti, P. et al. (2003) Immunol. Letters 88:171.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL10
• Uniprot:
  • Q28374()