IL-10 Antibody (JES3-12G8) - Azide and BSA Free

ELISA: IL-10 Antibody (JES3-12G8) [NBP2-27577] - Standard curve generated with Rat Anti-Human IL-10-UNLB ( Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (Clone JES3-12G8) followed by Streptavidin-HRP.

• Reactivity: Hu, Pm-Cm, V-Vi
• Applications: WB, ELISA, IHC, B/N, ELISA, ELISA(Det)
• Clone: JES3-12G8
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

IL-10 Antibody (JES3-12G8) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat IL-10 Antibody (JES3-12G8) - Azide and BSA Free (NBP2-27577) is a monoclonal antibody validated for use in IHC, WB and ELISA. Anti-IL-10 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: COS-expressed human IL-10
• Specificity: Human/Viral/Cynomolgus IL-10
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: IL10
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA Detection (Matched Antibody Pair)
  • ELISA 1:2000-1:4000
  • Enzyme-linked Immunosorbent Spot
  • Immunohistochemistry 1:10 - 1:2000
  • Immunohistochemistry-Frozen 1:10 - 1:2000
  • Immunohistochemistry-Paraffin
  • Neutralization
  • Western Blot
• Application Notes: May be paired with NBP2-77379 in a sandwich ELISA.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 1.0 mL of borate buffered saline, pH 8.2.
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for IL-10 Antibody (JES3-12G8) - Azide and BSA Free

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10 Antibody (NBP2-27577)(2)

Publications using NBP2-27577

• Andersson J, Abrams J, Bjork L et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994-09-01 [PMID: 7821961]
  
  Details:
  Human tonsil tissue from children with recurrent tonsillitis or infectious mononucleosis: 1. IMG-201A (IL-1B): IHC (F) and FA, see Tables 1, 2 and Fig 3.
• Aschenbrenner D, Quaranta M, Banerjee S, Ilott N Systems-level analysis of monocyte responses in inflammatory bowel disease identifies IL-10 and IL-1 cytokine networks that regulate IL-23 bioRxiv (ESPOT)

Bioinformatics

• Gene Symbol: IL10
• Uniprot:
  • NP_000563(Human)