IL-10 Antibody (3C12C12) - BSA Free

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Western Blot: IL-10 Antibody (3C12C12) [NB100-2185] - Analysis using IL10 mouse mAb against IL10 recombinant protein.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
3C12C12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

IL-10 Antibody (3C12C12) - BSA Free Summary

Description
Novus Biologicals Mouse IL-10 Antibody (3C12C12) - BSA Free (NB100-2185) is a monoclonal antibody validated for use in WB and ELISA. Anti-IL-10 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
Purified recombinant fragment of human IL-10 expressed in E. Coli.
Localization
Secreted
Specificity
IL-10 (3C12C12)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL10
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Western Blot 1:500-1:2000
Application Notes
Anti- IL-10 recognizes recombinant human IL-10 by Western Blot and ELISA.
Control
IL-10 Overexpression Lysate
Publications
Read Publication using NB100-2185.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Purity
Protein G purified

Alternate Names for IL-10 Antibody (3C12C12) - BSA Free

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukine 10 (IL-10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down- regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-10 Antibody (NB100-2185)(1)

We have publications tested in 1 confirmed species: Human.


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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IL-10 Antibody (NB100-2185) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional IL-10 Products

Research Areas for IL-10 Antibody (NB100-2185)

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Blogs on IL-10.


  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol IL10
Entrez