Novus Biologicals products are now on bio-techne.com

IL-10 Antibody (23738) [Unconjugated]

Images

 
Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-10 Monoclonal ...read more
Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL‑10 ...read more
Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, B/N
Clone
23738
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price

IL-10 Antibody (23738) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL‑10
Ser19-Asn178
Accession # P22301
Specificity
Detects human IL-10 in Western blots. In Western blots, this antibody shows approximately 5% cross-reactivity with recombinant mouse
IL-10.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
IL10
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization
  • Simple Western 10 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB217 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody (23738) [Unconjugated]

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

M6000B
Species: Mu
Applications: ELISA
6507-IL/CF
Species: Hu
Applications: BA
202-IL
Species: Hu
Applications: BA
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
M5000
Species: Mu
Applications: ELISA
7268-CT
Species: Hu
Applications: BA
DY413
Species: Mu
Applications: ELISA
201-LB
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
MAB208
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
NBP1-72042
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NB100-39002
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
NBP2-25208
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
MAB140
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
DCP00
Species: Hu
Applications: ELISA

Publications for IL-10 Antibody (MAB217)(29)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: Affinity Chromatography, Blocking, ELISA Development (Capture), Flow Cytometry, Luminex Development, Neutralization, Western Blot.


Filter By Application
Affinity Chromatography
(1)
Blocking
(1)
ELISA Development (Capture)
(1)
Flow Cytometry
(2)
Luminex Development
(1)
Neutralization
(16)
Western Blot
(1)
All Applications
Filter By Species
Human
(23)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using MAB217 Applications Species
A Dumigan, O Cappa, B Morris, J Sá Pessoa, R Calderon-G, G Mills, R Lancaster, D Simpson, A Kissenpfen, JA Bengoechea In vivo single-cell transcriptomics reveal Klebsiella pneumoniae skews lung macrophages to promote infection Embo Molecular Medicine, 2022-11-07;0(0):e16888. 2022-11-07 [PMID: 36337046] (Neutralization, Human) Neutralization Human
S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022-06-03;13(1):3121. 2022-06-03 [PMID: 35660734] (Neutralization, Human) Neutralization Human
T Fukasawa, A Yoshizaki, S Ebata, A Yoshizaki-, Y Asano, A Enomoto, K Miyagawa, Y Kazoe, K Mawatari, T Kitamori, S Sato Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model Elife, 2021-12-02;10(0):. 2021-12-02 [PMID: 34854378] (Neutralization, Human) Neutralization Human
Arpita Saha, Hamdi Ben Halima, Abhishek Saini, Juan Gallardo-Gonzalez, Nadia Zine, Clara Viñas et al. Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva Molecules 8/31/2020 [PMID: 32878151]
Amaro Nunes Duarte-Neto, Julio Croda, Carla Pagliari, Francisco Garcia Soriano, Antonio Carlos Nicodemo, Maria Irma Seixas Duarte Severe Leptospirosis Features in the Spleen Indicate Cellular Immunosuppression Similar to That Found in Septic Shock Frontiers in Immunology 2019-04-30 [PMID: 31114579]
K Brekke, M Sommerfelt, M Ökvist, AM Dyrhol-Rii, D Kvale The therapeutic HIV Env C5/gp41 vaccine candidate Vacc-C5 induces specific T cell regulation in a phase I/II clinical study BMC Infect. Dis, 2017-03-24;17(1):228. 2017-03-24 [PMID: 28340570] (Neutralization, Human) Neutralization Human
BD Hock, SA MacPherson, JL McKenzie Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells PLoS ONE, 2017-03-03;12(3):e0172858. 2017-03-03 [PMID: 28257435] (Neutralization, Human) Neutralization Human
Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages Sci Rep, 2016-08-25;6(0):31959. 2016-08-25 [PMID: 27558590] (Blocking, Human) Blocking Human
Lucio Roberto Castellano, Laurent Argiro, Helia Dessein, Alain Dessein, Marcos Vinícius da Silva, Dalmo Correia et al. Potential Use of Interleukin-10 Blockade as a Therapeutic Strategy in Human Cutaneous Leishmaniasis Journal of Immunology Research 2015-10-01 [PMID: 26495321]
Heine G, Drozdenko G, Grun J, Chang H, Radbruch A, Worm M Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts. Eur J Immunol, 2014-03-11;44(6):1615-21. 2014-03-11 [PMID: 24643722] (Neutralization, Human) Neutralization Human
Show All 29 Publications.

Reviews for IL-10 Antibody (MAB217) (0)

There are no reviews for IL-10 Antibody (MAB217). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-10 Antibody (MAB217) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IL-10 Products

Blogs on IL-10.


  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-10 Antibody (23738) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol IL10
Uniprot