IL-10 Antibody (148801)

Recombinant Porcine IL‑10 (Catalog # 693-PI) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Porcine IL‑10 Mono­clonal Antibody (Catalog # MAB6931). The ND50 is typically 0.05‑0.15 µg/mL.

• Reactivity: Po
• Applications: B/N
• Clone: 148801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-10 Antibody (148801) Summary

• Immunogen: E. coli-derived recombinant porcine IL-10
  Ser19-Asn175
  Accession # Q29055
• Specificity: Detects porcine IL‑10 in direct ELISAs. In direct ELISAs, 25-50% cross-reactivity with recombinant feline IL-10, recombinant canine IL-10, and recombinant guinea pig IL-10 is observed, approximately 10% cross-reactivity with recombinant human IL-10 is observed, less than 5% cross-reactivity with recombinant cotton rat IL-10 is observed, and no cross-reactivity with recombinant equine IL-10, recombinant mouse IL-10, recombinant rat IL-10, or recombinant viral IL-10 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL10
• Purity: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.05-0.15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody (148801)

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones which are dependent upon stimulation with antigen in the presence of antigen presenting cells (APC). Murine IL-10 is produced by Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, and LY-1⁺ (CD5⁺) and normal B cells. Human IL-10 has cross-species actvities and is active on mouse cells. Murine IL-10 is species-specific and does not act on human cells. Porcine IL-10 shares 71% and 78% amino acid sequence identitywith mouse and human IL-10, respectively. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, down‑regulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

1. Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10 Antibody (MAB6931)(3)

Publications using MAB6931

• Kandasamy S, Vlasova A, Fischer D, Kumar A, Chattha K, Rauf A, Shao L, Langel S, Rajashekara G, Saif L Differential Effects of Escherichia coli Nissle and Lactobacillus rhamnosus Strain GG on Human Rotavirus Binding, Infection, and B Cell Immunity. J Immunol, 2016;196(4):1780-9. 2016 [PMID: 26800875] (Neutralization, Porcine)
• Munoz-Gonzalez S, Ruggli N, Rosell R, Perez L, Frias-Leuporeau M, Fraile L, Montoya M, Cordoba L, Domingo M, Ehrensperger F, Summerfield A, Ganges L Postnatal persistent infection with classical Swine Fever virus and its immunological implications. PLoS ONE, 2015;10(5):e0125692. 2015 [PMID: 25938664] (Neutralization, Porcine)
• Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia. Intensive Care Med, 2007;33(6):1085-93. 2007 [PMID: 17457574] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL10
• Uniprot:
  • Q29055()