IL-10 Antibody (148801) [Alexa Fluor® 594]

• Reactivity: Po
• Applications: B/N
• Clone: 148801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

IL-10 Antibody (148801) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant porcine IL-10
  Ser19-Asn175
  Accession # Q29055
• Specificity: Detects porcine IL‑10 in direct ELISAs. In direct ELISAs, 25-50% cross-reactivity with recombinant feline IL-10, recombinant canine IL-10, and recombinant guinea pig IL-10 is observed, approximately 10% cross-reactivity with recombinant human IL-10 is obs
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL10
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Neutralization

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody (148801) [Alexa Fluor® 594]

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones which are dependent upon stimulation with antigen in the presence of antigen presenting cells (APC). Murine IL-10 is produced by Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, and LY-1⁺ (CD5⁺) and normal B cells. Human IL-10 has cross-species actvities and is active on mouse cells. Murine IL-10 is species-specific and does not act on human cells. Porcine IL-10 shares 71% and 78% amino acid sequence identitywith mouse and human IL-10, respectively. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, down‑regulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL10