IKK gamma Antibody (72C627)

Images

 
Western Blot: IKK gamma Antibody (72C627) [NB100-56542] - Analysis using IKK gamma (NEMO) antibody. Lysate from 1) human Jurkat and 2) mouse NIH 3T3 cells probed with IKK gamma antibody at 2 ug/ml.
Simple Western: IKK gamma Antibody (72C627) [NB100-56542] - Simple Western lane view shows a specific band for IKK gamma in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
Clone
72C627
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

IKK gamma Antibody (72C627) Summary

Immunogen
This antibody was raised against a His-tagged full-length human IKK3/IKKg protein.
Specificity
A 48 kDa band is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IKBKG
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Simple Western 1:200
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Antagonist
Positive Control
Jurkat Lysate (NB800-PC2)
NIH 3T3 Lysate (NB800-PC8)
Publications
Read Publications using
NB100-56542 in the following applications:

  • WB
    4 publications

Reactivity Notes

Cross reacts with Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for IKK gamma Antibody (72C627)

  • FIP-3IP2
  • FIP3NF-kappa-B essential modifier
  • FIP3P
  • I-kappa-B kinase subunit gamma
  • IkB kinase gamma subunit
  • IkB kinase subunit gamma
  • IkB kinase-associated protein 1
  • IKBKG
  • IKK gamma
  • IKKAP1
  • IKKG
  • IKK-gammaIPD2
  • incontinentia pigmenti
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
  • Inhibitor of nuclear factor kappa-B kinase subunit gamma
  • IP
  • IP1
  • NEMO
  • NEMOAMCBX1
  • NFkappaB essential modulator
  • NF-kappa-B essential modulator

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK3/g preferentially interacts with IKK2/b and is required for activation of IKK complex. IKK3/g is also known as NEMO (NF-kB Essential MOdulator). Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKa nor IKKb, but complexes directly with IKKg. This suggests that IKKg may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IKK gamma Antibody (NB100-56542)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(4)
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Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-56542 Applications Species
Konrath F, Witt J, Sauter TK, Kulms D. Identification of new IkBa complexes by an iterative experimental and mathematical modeling approach. PLoS Comput. Biol. 2014 Mar 01 [PMID: 24675998] (WB, Human)

Details:
kB epithelial carcinoma cell line, Fig 5E-G. The specificity of the Ikkgamma mAb was validated with an IP/WB assay by showing in WB that it recognized Ikkgamma IP'd from kB cells with a different Ikkgamma antibody (Fig 5G).
WB Human
Ren T, Takahashi Y, Liu X et al. HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains. Oncogene 2013 Dec 23 [PMID: 24362528] (WB, Human) WB Human
Siggs OM, Berger M, Krebs P et al. A mutation of Ikbkg causes immune deficiency without impairing degradation of IkappaB alpha. Proc Natl Acad Sci U S A. 2010 Feb 16 [PMID: 20133626]
Harikumar KB, Sung B, Pandey MK et al. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway. Mol Pharmacol. 2010 May [PMID: 20103608]

Details:
Monoclonal antibodies were used in western blot; human chronic myeloid leukemia KBM-5 cell line, Fig 4. 1. IKKB/IKK2 (IMG-129A) 2. IKKa/IKK1 (IMG-136A) 3. IKBalpha (IMG-127A).
Lamothe B, Campos AD, Webster WK et al. The RING domain and first zinc finger of TRAF6 coordinate signaling by interleukin-1, lipopolysaccharide, and RANKL. J Biol Chem. 2008 Sep 5 [PMID: 18617513] (WB, Mouse)

Details:
1. NEMO (IMG-324A): WB (HEk293 cells and mouse embryonic fibroblasts), Fig. 2A; 3A; 6A,B; 7A.
WB Mouse
Wunderlich FT, Luedde T, Singer S et al. Hepatic NF-kappa B essential modulator deficiency prevents obesity-induced insulin resistance but synergizes with high-fat feeding in tumorigenesis. Proc Natl Acad Sci U S A. 2008 Jan 29 [PMID: 18216263] (WB)

Details:
Ikkgamma NEMO (IMG-324A). WB: NEMO L-kO knockout and WT mouse liver cells, Fig S5.Note: The specificity of the antibody was validated in a NEMO L knockout mouse by WB, Fig S5.
WB
Han HS, Karabiyikoglu M, Kelly S et al. Mild hypothermia inhibits nuclear factor-kappaB translocation in experimental stroke. J Cereb Blood Flow Metab. 2003 May [PMID: 12771574]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IKK gamma Antibody (NB100-56542) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56542AF405
Alexa Fluor 488 Labeled NB100-56542AF488
Alexa Fluor 647 Labeled NB100-56542AF647
Alexa Fluor 700 Labeled NB100-56542AF700
Biotin Labeled NBP2-24816
DyLight 350 Labeled NB100-56542UV
DyLight 405 Labeled NB100-56542V
DyLight 488 Labeled NB100-56542G
DyLight 550 Labeled NB100-56542R
DyLight 650 Labeled NB100-56542C
DyLight 680 Labeled NB100-56542FR
DyLight 755 Labeled NB100-56542IR
HRP Labeled NB100-56542H

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Blogs on IKK gamma.

Regulating Immune Response Pathways with IKK beta
IKK beta, also known as IKK2, activates the NFkB complex by phosphorylating the NFkB inhibitor, IkBa. Several transcript variants, some protein-coding and some not, have been found for IKKB. The Nuclear Factor-kappa B (NF-kB) family of transcription f...  Read full blog post.

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Bioinformatics

Gene Symbol IKBKG
Entrez
Uniprot