IKEPP Antibody

Images

 
Western Blot: IKEPP Antibody [NB400-150] - Analysis on Lane 1: human kidney membrane lysate and Lane 2: mouse kidney membrane lysate.
Immunohistochemistry-Paraffin: IKEPP Antibody [NB400-150] - IKEPP was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human IKEPP polyclonal Antibody (Catalog # NB400-150) at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

IKEPP Antibody Summary

Immunogen
A synthetic peptide made to the C-terminus of mouse IKEPP. [UniProt# Q99MJ6]
Localization
Membrane-associated and cytoplasmic. In kidney, predominantly localized to the subapical compartment of proximal tubular cells.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDZD3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This IKEPP antibody is useful for Western blot, where a single band of ~60 kDa is detected. It is not useful for Immunocytochemistry/Immunofluorescence. Use in Immunohistochemistry reported in scientific literature (PMID 24391715) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB400-150 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse, human, and rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for IKEPP Antibody

  • FLJ22756
  • IKEPPPDZK2
  • intestinal and kidney enriched PDZ protein
  • Intestinal and kidney-enriched PDZ protein
  • Na(+)/H(+) exchange regulatory cofactor NHE-RF4
  • Na/Pi cotransporter C-terminal-associated protein 2
  • naPi-Cap2
  • Natrium-phosphate cotransporter IIa C-terminal-associated protein 2
  • NHERF4
  • NHERF-4
  • PDZ domain containing 2
  • PDZ domain containing 3
  • PDZ domain-containing protein 2
  • PDZ domain-containing protein 3
  • Sodium-hydrogen exchanger regulatory factor 4

Background

The Na+/H+ exchange regulatory factor (NHERF) family of PDZ-containing proteins comprises of four members including NHERF1/EBP50, NHERF2/E3KARP, NHERF3/PDZK1 and NHERF4/IKEPP/PDZK2; and among all, NHERF1/EBP50 and NHERF2 are most widely distributed. NHERF1/EBP50 and NHERF2 contain only two PDZ domains each, whereas, the highly homologous PDZK1 and IKEPP each contain four PDZ domains. IKEPP (intestinal and kidney enriched PDZ protein) expression is limited to kidney and gastrointestinal tissues, and at subcellular levels, it can be found in cellular membranes, cytoplasm as well as endosome membranes. In renal tissues, it predominantly localize to subapical compartment of proximal tubular cells, whereas, in intestinal epithelial cells, it localizes to ileal brush border as well as in a juxtanuclear compartment. Compared to the other NHERF family members, IKEPP has few known binding partners including guanylyl cyclase C, MAP17, NHE itself, type IIa Na-Pi co-transporter, MDR protein 2, OCT family members (OCT3, OCTN1 and OCTN2), CFTR and wild-type Cl/HCO3 exchanger down-regulated in adenoma (DRA). IKEPP acts as a regulatory protein which associates with GUCY2C and negatively modulates its heat-stable enterotoxin-mediated activation. It triggers SLC9A3 activity in the presence of elevated calcium ions through IKEPP-interactions with C-terminal region of SLC34A1, GUCY2C and SLC9A3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IKEPP Antibody (NB400-150)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, WB.


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Bioinformatics

Gene Symbol PDZD3
Entrez
Uniprot