IKEPP Antibody - BSA Free

Western Blot: IKEPP Antibody [NB400-150] - Analysis on Lane 1: human kidney membrane lysate and Lane 2: mouse kidney membrane lysate.

Immunohistochemistry-Paraffin: IKEPP Antibody [NB400-150] - IKEPP was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human IKEPP polyclonal Antibody (Catalog # NB400-150) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells in convoluted tubules.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, ICC/IF (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IKEPP Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IKEPP Antibody - BSA Free (NB400-150) is a polyclonal antibody validated for use in IHC and WB. Anti-IKEPP Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to the C-terminus of mouse IKEPP. [UniProt# Q99MJ6]
• Localization: Membrane-associated and cytoplasmic. In kidney, predominantly localized to the subapical compartment of proximal tubular cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NHERF4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry reported in scientific literature (PMID 24391715)
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Application Notes: This IKEPP antibody is useful for Western blot, where a single band of ~60 kDa is detected. It is not useful for Immunocytochemistry/Immunofluorescence. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for IKEPP Antibody - BSA Free

• FLJ22756
• IKEPPPDZK2
• intestinal and kidney enriched PDZ protein
• Intestinal and kidney-enriched PDZ protein
• Na(+)/H(+) exchange regulatory cofactor NHE-RF4
• Na/Pi cotransporter C-terminal-associated protein 2
• naPi-Cap2
• Natrium-phosphate cotransporter IIa C-terminal-associated protein 2
• NHERF4
• NHERF-4
• PDZ domain containing 2
• PDZ domain containing 3
• PDZ domain-containing protein 2
• PDZ domain-containing protein 3
• Sodium-hydrogen exchanger regulatory factor 4

Background

The Na+/H+ exchange regulatory factor (NHERF) family of PDZ-containing proteins comprises of four members including NHERF1/EBP50, NHERF2/E3KARP, NHERF3/PDZK1 and NHERF4/IKEPP/PDZK2; and among all, NHERF1/EBP50 and NHERF2 are most widely distributed. NHERF1/EBP50 and NHERF2 contain only two PDZ domains each, whereas, the highly homologous PDZK1 and IKEPP each contain four PDZ domains. IKEPP (intestinal and kidney enriched PDZ protein) expression is limited to kidney and gastrointestinal tissues, and at subcellular levels, it can be found in cellular membranes, cytoplasm as well as endosome membranes. In renal tissues, it predominantly localize to subapical compartment of proximal tubular cells, whereas, in intestinal epithelial cells, it localizes to ileal brush border as well as in a juxtanuclear compartment. Compared to the other NHERF family members, IKEPP has few known binding partners including guanylyl cyclase C, MAP17, NHE itself, type IIa Na-Pi co-transporter, MDR protein 2, OCT family members (OCT3, OCTN1 and OCTN2), CFTR and wild-type Cl/HCO3 exchanger down-regulated in adenoma (DRA). IKEPP acts as a regulatory protein which associates with GUCY2C and negatively modulates its heat-stable enterotoxin-mediated activation. It triggers SLC9A3 activity in the presence of elevated calcium ions through IKEPP-interactions with C-terminal region of SLC34A1, GUCY2C and SLC9A3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IKEPP Antibody (NB400-150)(1)

Publication using NB400-150

• Hovakimyan M, Meyer A, Lukas J et al. Olfactory Deficits in Niemann-Pick Type C1 (NPC1) Disease. PLoS One 2013-12-31 [PMID: 24391715] (IF/IHC, WB, Mouse)

Bioinformatics

• Gene Symbol: NHERF4
• Entrez:
  • 79849(Human)
  • 170761(Mouse)
  • 500986(Rat)
• Uniprot:
  • Q99MJ6(Mouse)