| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P, KO |
| Clone | 6A920 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 350 |
| Immunogen | A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen. |
| Specificity | A 40 kDa band is observed. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NFKBIA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
![IL-6 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
| Allophycocyanin Labeled | NB100-56507APC | |
| Biotin Labeled | NBP2-24815 | |
| DyLight 405 Labeled | NB100-56507V | |
| DyLight 488 Labeled | NB100-56507G | |
| DyLight 550 Labeled | NB100-56507R | |
| DyLight 650 Labeled | NB100-56507C | |
| DyLight 680 Labeled | NB100-56507FR | |
| DyLight 755 Labeled | NB100-56507IR | |
| HRP Labeled | NB100-56507H | |
| PE Labeled | NB100-56507PE | |
| PerCP Labeled | NB100-56507PCP |
Diseases for IkB-alpha Antibody (NB100-56507UV)Discover more about diseases related to IkB-alpha Antibody (NB100-56507UV).
| Pathways for IkB-alpha Antibody (NB100-56507UV)View related products by pathway.
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Sample collection from mammalian culture cells for kinomic analysis By Jamshed Arslan Pharm.D., PhD.IntroductionKinome describes kinases, and kinomics refers to the kinase signaling. Studying the effects of reagent (exogenously applied growth factor or inhibitor) on kinase activit... Read full blog post. |
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The effects of curcumin on IKB Alpha and the NFkB signaling pathway The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta. These kinases are at the core of the NFkB signaling cascade. The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through... Read full blog post. |
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Inhibitor kappa B-alpha (IkappaB-alpha) The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NFKBIA |
![Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize2/NFkB-p105-p50-Antibody-(2J10D7)-Western-Blot-NB100-56583-img0004.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review](http://images.novusbio.com/fullsize2/NFkB-p105-p50-Antibody-(2J10D7)-Immunohistochemistry-Paraffin-NB100-56583-img0009.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize2/NFkB-p105-p50-Antibody-(2J10D7)-Flow-Cytometry-NB100-56583-img0008.jpg)
![ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Standard curve using the internal RelA standard.](http://images.novusbio.com/fullsize2/Human--Mouse--Rat-RelA-NFkB-p65-ELISA-Kit-(Colorimetric)-ELISA-NBP2-29661-img0004.jpg)
![ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Nuclear translocation of NF-kB p65 with LPS in Jurkat cells. Jurkat cells, grown to a density of 2X10(6) cells/ml, were treated with LPS (15 ug/ml). The cells were harvested at various indicated time points, and cell lysates were tested in Western analysis using NB100-56712 and also in this ELISA Kit.](http://images.novusbio.com/fullsize2/Human--Mouse--Rat-RelA-NFkB-p65-ELISA-Kit-(Colorimetric)-ELISA-NBP2-29661-img0005.jpg)
![ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Immunofluorescence indicates NF-kB p65 subunit was expressed exclusively in AA-G groups and AST-G cytoplasm. High glucose stimulation impact on TSMCs in NF-kB pathway. (A) NF-kB p65 protein subunit expression in TSMCs in each group. (B) NF-kB p65 subunit immunofluorescence. Each number denotes different subunits. Alexa 488 green served as secondary antibodies.Citation: Hara K, Hamada C, Wakabayashi K, Kanda R, Kaneko K, Horikoshi S, et al. (2017) Scavenging of reactive oxygen species by astaxanthin inhibits epithelial-mesenchymal transition in high glucose-stimulated mesothelial cells. PLoS ONE 12(9): e0184332. https://doi.org/10.1371/journal.pone.0184332](http://images.novusbio.com/fullsize2/Human--Mouse--Rat-RelA-NFkB-p65-ELISA-Kit-(Colorimetric)-ELISA-NBP2-29661-img0006.jpg)
![Western Blot: Synaptotagmin 1 Antibody [NBP1-91499] - Synaptotagmin 1 in bovine oocytes. Image from confirmed customer review.](http://images.novusbio.com/fullsize2/Synaptotagmin-1-Antibody-Western-Blot-NBP1-91499-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Synaptotagmin 1 Antibody [NBP1-91499] - Sample Type: outer mouse plexiform layer, Red: Primary, Blue: DAPI, Primary Dilution: 1:200, Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L), Secondary Dilution: 1:200, Image Submitted by: David Zenisek, Yale University.](http://images.novusbio.com/fullsize2/Synaptotagmin 1 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91499-img0005.jpg)
![Western Blot: Synaptotagmin 1 Antibody [NBP1-91499] - Mouse Spleen Lysate 1ug/ml Gel Concentration 12%](http://images.novusbio.com/fullsize2/Synaptotagmin 1 Antibody-Western Blot-NBP1-91499-img0001.jpg)
![Western Blot: WNK1 Antibody [NB600-225] - Detection of the short form of WNK1 in F-11 cell lysate.](http://images.novusbio.com/fullsize2/WNK1 Antibody-Western Blot-NB600-225-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: WNK1 Antibody [NB600-225] - WNK1 antibody was tested in HEK293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/WNK1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-225-img0005.jpg)
![Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GRASP65 monoclonal antibody.](http://images.novusbio.com/fullsize2/GRASP65-Antibody-(5G8)-Western-Blot-NBP2-02665-img0009.jpg)
![Immunocytochemistry: GRASP65 Antibody (5G8) [NBP2-02665] - Golgi localization in human Wi26 cells. Works for ICC on MeOH and PFA-fixed cells 1:100-1:200 dilution WB on human lung fibroblasts 1:2000 in TBST (membrane blocking with 5% skimmed milk). This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/GRASP65 Antibody (5G8)-Immunocytochemistry-NBP2-02665-img0010.jpg)
![Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human prostate tissue using anti-GRASP65 mouse monoclonal antibody.](http://images.novusbio.com/fullsize2/GRASP65-Antibody-(5G8)-Immunohistochemistry-Paraffin-NBP2-02665-img0007.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Western blot analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/fullsize2/c-jun-Antibody-(5B1)-Western-Blot-NBP2-37576-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Immunofluorescence analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize2/c-jun-Antibody-(5B1)-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry: c-jun Antibody (5B1) [NBP2-37576] - Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/c-jun-Antibody-(5B1)-Immunohistochemistry-NBP2-37576-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - CD40-transfected L cells stained.](http://images.novusbio.com/fullsize2/CD40-TNFRSF5-Antibody-(mAb89)-Immunocytochemistry-Immunofluorescence-DDX0131P-100-img0003.jpg)
![Immunohistochemistry: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - IHC staining on Bouin paraffinlymph node section. HumanCD40 stained with mAb 89.](http://images.novusbio.com/fullsize2/CD40-TNFRSF5-Antibody-(mAb89)-Immunohistochemistry-DDX0131P-100-img0004.jpg)
![Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12](http://images.novusbio.com/fullsize2/ERK2 Antibody (SZ25-01)-Western Blot-NBP2-67360-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/ERK2-Antibody-(SZ25-01)-Immunocytochemistry-Immunofluorescence-NBP2-67360-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/ERK2-Antibody-(SZ25-01)-Immunohistochemistry-Paraffin-NBP2-67360-img0007.jpg)
![Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1ug/mL for 16 hours (right lane) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize2/iNOS-Antibody-Western-Blot-NB300-605-img0015.jpg)
![Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Image from verified customer review.](http://images.novusbio.com/fullsize2/iNOS-Antibody-Western-Blot-NB300-605-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in A549 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a iNOS polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. iNOS staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize2/iNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB300-605-img0012.jpg)
![Western Blot: ARHGEF7 Antibody [NBP1-88650] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize2/ARHGEF7-Antibody-Western-Blot-NBP1-88650-img0007.jpg)
![Immunohistochemistry-Paraffin: ARHGEF7 Antibody [NBP1-88650] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize2/ARHGEF7-Antibody-Immunohistochemistry-Paraffin-NBP1-88650-img0010.jpg)
![Immunohistochemistry-Paraffin: ARHGEF7 Antibody [NBP1-88650] - Staining of human duodenum shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/ARHGEF7-Antibody-Immunohistochemistry-Paraffin-NBP1-88650-img0002.jpg)
![Western Blot: ASCC1 Antibody (6G5) [NBP2-01345] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCC1.](http://images.novusbio.com/fullsize/ASCC1 Antibody (6G5)-Western Blot-NBP2-01345-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ASCC1 Antibody (6G5) [NBP2-01345] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ASCC1.](http://images.novusbio.com/fullsize/ASCC1 Antibody (6G5)-Immunocytochemistry-Immunofluorescence-NBP2-01345-img0006.jpg)
![Immunohistochemistry-Paraffin: ASCC1 Antibody (6G5) [NBP2-01345] - Staining of paraffin-embedded Human tonsil using anti-ASCC1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ASCC1 Antibody (6G5)-Immunohistochemistry-Paraffin-NBP2-01345-img0005.jpg)
![Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml.](http://images.novusbio.com/fullsize2/IKK-beta-Antibody-(10AG2)-Western-Blot-NB100-56509-img0007.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [NB100-56509] - Human gall bladder probed with IKKbeta antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize2/IKK-beta-Antibody-(10AG2)-Immunohistochemistry-Paraffin-NB100-56509-img0006.jpg)
![Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of PE conjugate of NB100-56509. An intracellular stain was performed on Jurkat cells with IKK Beta antibody (10AG2) NB100-56509PE (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized wi](http://images.novusbio.com/fullsize2/IKK-beta-Antibody-(10AG2)-Flow-Cytometry-NB100-56509-img0012.jpg)
![Western Blot: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Analysis using Azide Free version of NB100-56704. IKK in Daudi cell lysate using IKKa monoclonal antibody at 1 ug/mL.](http://images.novusbio.com/fullsize2/IKK-alpha-Antibody-(14A231)-Western-Blot-NB100-56704-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilution of 1:10 was used. Image objective 40X.](http://images.novusbio.com/fullsize2/IKK-alpha-Antibody-(14A231)-Immunocytochemistry-Immunofluorescence-NB100-56704-img0008.jpg)
![Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Analysis using Azide Free version of NB100-56704. FFPE human kidney stained with IKKa antibody at 5 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10 min.](http://images.novusbio.com/fullsize2/IKK-alpha-Antibody-(14A231)-Immunohistochemistry-Paraffin-NB100-56704-img0011.jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody.](http://images.novusbio.com/fullsize2/COX-2-Antibody-Immunohistochemistry-Paraffin-NB100-689-img0009.jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded human colon carcinoma stained with COX2 antibody.](http://images.novusbio.com/fullsize2/COX-2-Antibody-Immunohistochemistry-Paraffin-NB100-689-img0004.jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded human colon carcinoma stained with COX2 antibody.](http://images.novusbio.com/fullsize2/COX-2-Antibody-Immunohistochemistry-Paraffin-NB100-689-img0005.jpg)
