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IkB-alpha Antibody (6A920) [DyLight 350]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, KO
Clone
6A920
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 350

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-56507AF405
Alexa Fluor 488
NB100-56507AF488
Alexa Fluor 647
NB100-56507AF647
Alexa Fluor 700
NB100-56507AF700
Allophycocyanin
NB100-56507APC
Biotin
NBP2-24815
DyLight 405
NB100-56507V
DyLight 488
NB100-56507G
DyLight 550
NB100-56507R
DyLight 650
NB100-56507C
DyLight 680
NB100-56507FR
DyLight 755
NB100-56507IR
FITC
NB100-56507F
HRP
NB100-56507H
Janelia Fluor 549
NB100-56507JF549
Janelia Fluor 646
NB100-56507JF646
PE
NB100-56507PE
PerCP
NB100-56507PCP
Unconjugated
NB100-56507
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IkB-alpha Antibody (6A920) [DyLight 350] Summary

Immunogen
A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.
Specificity
A 40 kDa band is observed.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NFKBIA
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 23840265).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for IkB-alpha Antibody (6A920) [DyLight 350]

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • NFKBI
  • NFKBIA
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Nuclear translocation of NF-kB p65 with LPS in Jurkat cells. Jurkat cells, grown to a density of 2X10(6) cells/ml, were treated with LPS (15 ug/ml). The cells were harvested at various indicated time points, and cell lysates were tested in Western analysis using NB100-56712 and also in this ELISA Kit.
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NBP2-67471
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Simple Western lane view shows lysates of CH‑1 mouse B cell lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Rel at approximately 71 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse c‑Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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IKK epsilon/IKBKE
Antibody (346909) [Un ...
MAB3199
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line, Ramos human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat IKK epsilon Monoclonal Antibody (Catalog # MAB3199) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IKK epsilon at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
IKK epsilon was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat IKK epsilon Monoclonal Antibody (Catalog # MAB3199) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IKK epsilon was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human/Mouse/Rat IKK epsilon Monoclonal Antibody (Catalog # MAB3199) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-56507APC
Biotin Labeled NBP2-24815
DyLight 405 Labeled NB100-56507V
DyLight 488 Labeled NB100-56507G
DyLight 550 Labeled NB100-56507R
DyLight 650 Labeled NB100-56507C
DyLight 680 Labeled NB100-56507FR
DyLight 755 Labeled NB100-56507IR
HRP Labeled NB100-56507H
PE Labeled NB100-56507PE
PerCP Labeled NB100-56507PCP
Learn about Custom Antibody Labeling

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Blogs on IkB-alpha.

Check out the latest blog posts on IkB-alpha.
The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.
Inhibitor kappa B-alpha (IkappaB-alpha)
The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess...  Read full blog post.

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Bioinformatics

Gene Symbol NFKBIA