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IGF-II R/IGF2R Antibody (MEM-240) - BSA Free

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Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Detection of CD222 in human peripheral blood with anti-CD222 (MEM-240) purified, GAM-APC.
Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in ...read more
Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP
Clone
MEM-240
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

IGF-II R/IGF2R Antibody (MEM-240) - BSA Free Summary

Additional Information
Clone MEM-240 was used by HLDA to establish CD designation.
Immunogen
Recombinant Vaccinia virus encoding IGF-II R/IGF2R.
Specificity
The antibody MEM-238 recognizes an extracellular epitope between amino acids 698-1262 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGF2R
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 2-6 ug/ml
  • Immunoprecipitation
  • Western Blot
Application Notes
Flow cytometry: Extracellular and intracellular staining. Recommended dilution: 2-6 ug/ml. Western blotting: non-reducing conditions. Recommended dilution: 1-2 ug/ml.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for IGF-II R/IGF2R Antibody (MEM-240) - BSA Free

  • 300 kDa mannose 6-phosphate receptor
  • cation-independent mannose-6 phosphate receptor
  • cation-independent mannose-6-phosphate receptor
  • CD222 antigen
  • CD222
  • CI Man-6-P receptor
  • CIMPR
  • CI-MPR
  • IGF2R
  • IGF-II R
  • IGF-II receptor
  • IGFIIR
  • IGF-IIR
  • insulin-like growth factor 2 receptorM6P/IGF2R
  • Insulin-like growth factor II receptor
  • M6P/IGF2 receptor
  • M6PR
  • M6P-R
  • MPR 300
  • MPR1
  • MPRI
  • MPRIM6PR

Background

CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IGF2R