IGF-II R/IGF2R Antibody (MEM-238) [FITC]

Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [FITC] [NB100-63599] - Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238)[NB100-63599]- Staining human peripheral blood monocytes following permeablisation with Leucoperm (Fix & Perm) (BUF09).

• Reactivity: Hu, Pm
• Applications: WB, Flow, IP
• Clone: MEM-238
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

IGF-II R/IGF2R Antibody (MEM-238) [FITC] Summary

• Immunogen: Recombinant Vaccinia virus encoding CD222.
• Epitope: Recognizes an epitope between domains 2 and 5.
• Marker: Late Endosome Marker
• Specificity: The antibody MEM-238 recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal ompartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGF2R
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
• Theoretical MW: 250 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS and 0.2% (w/v) high grade BSA (protease free)
• Preservative: 0.09% Sodium Azide
• Purity: Size Exclusion Chromatography

Alternate Names for IGF-II R/IGF2R Antibody (MEM-238) [FITC]

• 300 kDa mannose 6-phosphate receptor
• cation-independent mannose-6 phosphate receptor
• cation-independent mannose-6-phosphate receptor
• CD222 antigen
• CD222
• CI Man-6-P receptor
• CIMPR
• CI-MPR
• IGF2R
• IGF-II R
• IGF-II receptor
• IGFIIR
• IGF-IIR
• insulin-like growth factor 2 receptorM6P/IGF2R
• Insulin-like growth factor II receptor
• M6P/IGF2 receptor
• M6PR
• M6P-R
• MPR 300
• MPR1
• MPRI
• MPRIM6PR

Background

CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGF-II R/IGF2R Antibody (NB100-63599)(1)

Publication using NB100-63599

• Leksa V, Godar S, Cebecauer M et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration J Biol Chem 2002-10-25 [PMID: 12189157]
  
  Details:
  This citation used the FITC version of this antibody.

Bioinformatics

• Gene Symbol: IGF2R