IGF-I R/IGF1R Antibody


Western Blot: IGF-I R Antibody [NBP1-77679] - Analysis of IGF-1R in 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. SK-BR-3 and 8. COS7
Immunocytochemistry/ Immunofluorescence: IGF-I R Antibody [NBP1-77679] - IGF-1 R antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77679] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody ...read more
Simple Western: IGF-I R Antibody [NBP1-77679] - Simple Western lane view shows a specific band for IGF-1 R in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
1.05 mg/ml

IGF-I R/IGF1R Antibody Summary

A synthetic peptide made to a C-terminal portion of the human IGF1 Receptor protein (between residues 1300-1367) [UniProt P08069].
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This IGFR1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot where a band is seen ~100 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
NIH 3T3 Whole Cell Lysate
HeLa Whole Cell Lysate
IGF-I R/IGF1R Knockout HeLa Cell Lysate

Reactivity Notes

Human, mouse, monkey and rat.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
1.05 mg/ml
Immunogen affinity purified

Alternate Names for IGF-I R/IGF1R Antibody

  • CD221 antigen
  • CD221
  • EC 2.7.10
  • EC
  • IGF1R
  • IGF-1R
  • IGF-I R
  • IGF-I receptor
  • IGF-IR
  • IGFR
  • insulin-like growth factor 1 receptor
  • Insulin-like growth factor I receptor
  • JTK13
  • MGC142170
  • MGC142172
  • MGC18216
  • soluble IGF1R variant 1
  • soluble IGF1R variant 2


Insulin-like growth factor receptor 1 (IGFR1) is a tyrosine kinase receptor that is important in embryonic development. IGFR1 has effects in breast, prostate, gastric and lung cancers, as it can be a player in drug resistance. It also affects the flow of nutrients from mother to placenta to fetus during development. IGFR1 seems to greatly influence the rate of growth of fetuses and newborn babies; when it is not appropriately expressed or regulated, fetal growth restriction occurs. The major activity of IGFR1 is to mediate the signaling of growth signals to effect downstream growth patterns.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IGF-I R/IGF1R Antibody (NBP1-77679). (Showing 1 - 1 of 1 FAQ).

  1. Our customer would like to perform IHC with samples from Rat. She has questions regarding antibodies of choice: 1. Is it possible to use NB400-142 for IHC? 2. I am having difficulty of choosing between NBP1-77679 and NBP1-77680. Would you please help suggest? 3. Although it is described as IHC (-) on the product page of NBP1-07034, This antibody did not work in IHC with mouse tissue is also described. How about Rat samples? Is it possible that the antibody can work in IHC with rat tissues?
    • To answer your questions about NB400-142, this antibody has not been validated for IHC and we cannot guarantee that it will work. Both NBP1-77679 and NBP1-77680 have been validated for use in rat and IHC. The difference between these 2 antibodies is the immunogen as they were raised against different regions of the protein. Please point out the immunogen to your customer and have them decide what would be best for their research. NBP1-07034 does not work in IHC, it is only guaranteed for Western blot in human, mouse and rat. When an application is followed by a (-) I would not recommend using this application in any species.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77679AF350
Alexa Fluor 405 NBP1-77679AF405
Alexa Fluor 488 NBP1-77679AF488
Alexa Fluor 532 NBP1-77679AF532
Alexa Fluor 594 NBP1-77679AF594
Alexa Fluor 647 NBP1-77679AF647
Alexa Fluor 700 NBP1-77679AF700
Alexa Fluor 750 NBP1-77679AF750
Biotin NBP1-77679B
DyLight 350 NBP1-77679UV
DyLight 405 NBP1-77679V
DyLight 488 NBP1-77679G
DyLight 550 NBP1-77679R
DyLight 594 NBP1-77679DL594
DyLight 650 NBP1-77679C
DyLight 680 NBP1-77679FR
DyLight 755 NBP1-77679IR
FITC NBP1-77679F
HRP NBP1-77679H
Janelia Fluor 549 NBP1-77679JF549
Janelia Fluor 646 NBP1-77679JF646

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Gene Symbol IGF1R