IGF-I R/IGF1R Antibody - BSA Free

Western Blot: IGF-I R Antibody [NBP1-77679] - Analysis of IGF-1R in 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. SK-BR-3 and 8. COS7

Immunocytochemistry/Immunofluorescence: IGF-I R Antibody [NBP1-77679] - IGF-1 R antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77679] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody (NBP1-77679). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.

Simple Western: IGF-I R Antibody [NBP1-77679] - Simple Western lane view shows a specific band for IGF-1 R in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.05 mg/ml

IGF-I R/IGF1R Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human IGF1 Receptor protein (between residues 1300-1367) [UniProt P08069].
• Localization: Membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGF1R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:100
  • Western Blot 1 ug/ml
• Application Notes: This IGFR1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot where a band is seen ~100 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 103 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  NIH 3T3 Whole Cell Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.05 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IGF-I R/IGF1R Antibody - BSA Free

• CD221 antigen
• CD221
• EC 2.7.10
• EC 2.7.10.1
• IGF1R
• IGF-1R
• IGF-I R
• IGF-I receptor
• IGFIR
• IGF-IR
• IGFR
• insulin-like growth factor 1 receptor
• Insulin-like growth factor I receptor
• JTK13
• MGC142170
• MGC142172
• MGC18216
• soluble IGF1R variant 1
• soluble IGF1R variant 2

Background

Insulin-like growth factor receptor 1 (IGFR1) is a tyrosine kinase receptor that is important in embryonic development. IGFR1 has effects in breast, prostate, gastric and lung cancers, as it can be a player in drug resistance. It also affects the flow of nutrients from mother to placenta to fetus during development. IGFR1 seems to greatly influence the rate of growth of fetuses and newborn babies; when it is not appropriately expressed or regulated, fetal growth restriction occurs. The major activity of IGFR1 is to mediate the signaling of growth signals to effect downstream growth patterns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGF-I R/IGF1R Antibody (NBP1-77679)(3)

Publications using NBP1-77679

• Xu G, Chen J, Lu B, Sethupathy P et Al. Verapamil Prevents Decline of IGF-I in Subjects With Type 1 Diabetes and Promotes ?-Cell IGF-I Signaling Diabetes 2023-07-26 [PMID: 37494660]
• Kalantzakos TJ, Sebel LE, Trussler J et al. MicroRNA Associated with the Invasive Phenotype in Clear Cell Renal Cell Carcinoma: Let-7c-5p Inhibits Proliferation, Migration, and Invasion by Targeting Insulin-like Growth Factor 1 Receptor Biomedicines 2022-09-28 [PMID: 36289686] (Simple Western, Human)
• Liang Z, Zhang Z, Zhang Q et al. The proprotein convertase furin regulates the development of thymic epithelial cells to ensure central immune tolerance iScience 2022-10-21 [PMID: 36274943]

FAQs for IGF-I R/IGF1R Antibody (NBP1-77679). (Showing 1 - 1 of 1 FAQs).

1. Our customer would like to perform IHC with samples from Rat. She has questions regarding antibodies of choice: 1. Is it possible to use NB400-142 for IHC? 2. I am having difficulty of choosing between NBP1-77679 and NBP1-77680. Would you please help suggest? 3. Although it is described as IHC (-) on the product page of NBP1-07034, This antibody did not work in IHC with mouse tissue is also described. How about Rat samples? Is it possible that the antibody can work in IHC with rat tissues?
   • To answer your questions about NB400-142, this antibody has not been validated for IHC and we cannot guarantee that it will work. Both NBP1-77679 and NBP1-77680 have been validated for use in rat and IHC. The difference between these 2 antibodies is the immunogen as they were raised against different regions of the protein. Please point out the immunogen to your customer and have them decide what would be best for their research. NBP1-07034 does not work in IHC, it is only guaranteed for Western blot in human, mouse and rat. When an application is followed by a (-) I would not recommend using this application in any species.

Bioinformatics

• Gene Symbol: IGF1R
• Entrez:
  • 3480(Human)
  • 101883(Mouse)
• Uniprot:
  • P08069()