IGFBP-5 Antibody

Images

 
IGFBP‑5 was detected in perfusion fixed frozen sections of mouse ovary (follicle with oocyte) using 5 µg/mL Goat Anti-Mouse IGFBP‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

IGFBP-5 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-5
Val15-Glu271
Accession # Q3UQV0
Specificity
Detects mouse IGFBP-5 in ELISAs and Western blots. In sandwich ELISAs, less than 3% cross-reactivity with recombinant human IGFBP-5 is observed, and less than 0.3% cross-reactivity with recombinant mouse (rm) IGF‑I, rmIGF-II, rmIGFBP-1, rmIGFBP-2, rmIGFBP-3, and rmIGFBP-6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGFBP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Mouse IGFBP-5 Antibody (Catalog # AF578)
ELISA Detection: Mouse IGFBP-5 Biotinylated Antibody (Catalog # BAF578)
Standard: Recombinant Mouse IGFBP-5 (Catalog # 578-B5)
Publications
Read Publications using
AF578 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGFBP-5 Antibody

  • IBP5IBP-5
  • IGF-binding protein 5
  • IGFBP5
  • IGFBP-5
  • insulin-like growth factor binding protein 5
  • insulin-like growth factor-binding protein 5

Background

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Mouse IGFBP-5 cDNA encodes a 271 amino acid (aa) residue precursor protein with a putative 19 aa residue signal peptide that is processed to generate the 252 aa residue mature protein. Mouse, human and rat IGFBP-5 share 97% identity. IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracelluar matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment.

  1. James, P.L. et al. (1993) J. Biol. Chem. 268:22305.
  2. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  3. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-80549
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
MAB8761
Species: Hu
Applications: WB, ELISA(Cap), ELISA(Det), ELISA(Sta)
MAB391
Species: Hu, Mu
Applications: WB, Flow, IHC, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF1067
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, Neut
AF1067
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, Neut
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
MAB1419
Species: Hu, Rt
Applications: IHC, CyTOF-ready, ICC, ICFlow
AF1360
Species: Mu
Applications: WB, IHC

Publications for IGFBP-5 Antibody (AF578)(3)

Reviews for IGFBP-5 Antibody (AF578) (0)

There are no reviews for IGFBP-5 Antibody (AF578). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IGFBP-5 Antibody (AF578) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF578

Additional IGFBP-5 Products

Bioinformatics Tool for IGFBP-5 Antibody (AF578)

Discover related pathways, diseases and genes to IGFBP-5 Antibody (AF578). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IGFBP-5 Antibody (AF578)

Discover more about diseases related to IGFBP-5 Antibody (AF578).
 

Pathways for IGFBP-5 Antibody (AF578)

View related products by pathway.

PTMs for IGFBP-5 Antibody (AF578)

Learn more about PTMs related to IGFBP-5 Antibody (AF578).

Blogs on IGFBP-5

There are no specific blogs for IGFBP-5, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IGFBP-5 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol IGFBP5
Uniprot