IGF-II R/IGF2R Antibody (2G11) - BSA Free


Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunohistochemistry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining of human testis using NB300-514 antibody at 1:100.
Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - An intracellular stain was performed on MCF7 cells with IGF-II R/IGF2R [2G11] Antibody NB300-514AF647 (blue) and a matched isotype control (orange). Cells ...read more
Immunocytochemistry/ Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei ...read more
Immunocytochemistry/ Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - HMVEC Cells
Immunocytochemistry/ Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining in Hela Cells. Mannose 6-Phosphate Receptor staining (green), F-Actin staining with ...read more
Immunocytochemistry/ Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6 ...read more
Flow Cytometry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Mannose 6 Phosphate Receptor antibody (dark blue). ...read more

Product Details

Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
BSA Free
1.0 mg/ml

Order Details

IGF-II R/IGF2R Antibody (2G11) - BSA Free Summary

Purified bovine Mannose 6 Phosphate Receptor (Cation independent) [UniProt# P08169]
In the extracellular domain of Mannose 6 Phosphate Receptor (Cation independent).
Late Endosome Marker
IgG2a Kappa
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:250
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1 ug/ml
Application Notes
By Western Blot, a 300 kDa protein representing Mannose 6 Phosphate Receptor is seen in HeLa cell extract under non-reducing conditions. Immunofluorescence staining of Mannose 6 Phosphate Receptor in HeLa cells with this antibody results in perinuclear staining. This antibody is also useful in Flow Cytometry, Immunoprecipitation, ELISA and Immunohistochemistry-on paraffin embedded sections. This antibody is CyTOF ready.
Read Publications using
NB300-514 in the following applications:

  • 1 publication
  • 6 publications
  • IHC
    2 publications
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 9119492). Does not react with Chinese hamster ovary cell Mannose 6 Phosphate Receptor (PMID: 8163521).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein A purified

Alternate Names for IGF-II R/IGF2R Antibody (2G11) - BSA Free

  • 300 kDa mannose 6-phosphate receptor
  • cation-independent mannose-6 phosphate receptor
  • cation-independent mannose-6-phosphate receptor
  • CD222 antigen
  • CD222
  • CI Man-6-P receptor
  • CI-MPR
  • IGF2R
  • IGF-II R
  • IGF-II receptor
  • insulin-like growth factor 2 receptorM6P/IGF2R
  • Insulin-like growth factor II receptor
  • M6P/IGF2 receptor
  • M6PR
  • M6P-R
  • MPR 300
  • MPR1
  • MPRI


Mannose 6 phosphate is a sequence tag that plays a pivotal role in delivering proteins to the lysosome. Specifically, the mannose 6 phosphate sequence is tagged to N-linked oligosaccharides of lysosomal hydrolases as they pass through the cis-Golgi network. Once the lysosomal enzymes are tagged with the M6P sequence, they are destined to the late endosome by way of vesicular transport. After a low pH dissociates the M6P Receptor from its ligand, the M6P sequences are recycled. Additionally, M6P is bound by lectin in the immune system, and is converted to fructose 6-phosphate by mannose phosphate isomerase.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGF-II R/IGF2R Antibody (NB300-514)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC, WB.

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Showing Publications 1 - 10 of 10.
Publications using NB300-514 Applications Species
Aguilar-GonzAlez A, GonzAlez-Correa JE, Barriocanal-Casado E et al. Isogenic GAA-KO Murine Muscle Cell Lines Mimicking Severe Pompe Mutations as Preclinical Models for the Screening of Potential Gene Therapy Strategies International journal of molecular sciences 2022-06-04 [PMID: 35682977] (FLOW, Mouse) FLOW Mouse
Muzio L, Sirtori R, Gornati D et al. Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis Nat Commun 2020-07-31 [PMID: 32737286] (IHC, Mouse) IHC Mouse
Kakegawa W, Katoh A, Narumi S et al. Optogenetic Control of Synaptic AMPA Receptor Endocytosis Reveals Roles of LTD in Motor Learning Neuron 2018-08-08 [PMID: 30122381] (ICC/IF, Mouse) ICC/IF Mouse
Takahashi K, Mashima H, Miura K et al. Disruption of Small GTPase Rab7 Exacerbates the Severity of Acute Pancreatitis in Experimental Mouse Models. Sci Rep. 2017-06-06 [PMID: 28588238] (IHC, WB, Mouse) IHC, WB Mouse
Ganley IG, Carroll K, Bittova L, Pfeffer S. Rab9 GTPase regulates late endosome size and requires effector interaction for its stability. Mol Biol Cell. 2004-12-01 [PMID: 15456905] (ICC/IF, WB, Human) ICC/IF, WB Human
Arighi CN, Hartnell LM, Aguilar RC et al. Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. J Cell Biol. 2004-04-01 [PMID: 15078903] (ICC/IF, Human) ICC/IF Human
Valdez AC, Cabaniols JP, Brown MJ, Roche PA. Syntaxin 11 is associated with SNAP-23 on late endosomes and the trans-Golgi network. J Cell Sci. 1999-03-01 [PMID: 10036234] (ICC/IF, Human) ICC/IF Human
Barker LP, George KM, Falkow S, Small PL. Differential trafficking of live and dead Mycobacterium marinum organisms in macrophages. Infect Immun. 1997-04-01 [PMID: 9119492] (ICC/IF, Mouse) ICC/IF Mouse
Heinzen RA, Scidmore MA, Rockey DD, Hackstadt T. Differential interaction with endocytic and exocytic pathways distinguish parasitophorous vacuoles of Coxiella burnetii and Chlamydia trachomatis. Infect Immun. 1996-03-01 [PMID: 8641784] (ICC/IF, Human) ICC/IF Human
Dintzis SM et al. Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor. J Biol Chem 269:12159-66. 1994-01-01 [PMID: 8163521]

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ICC/IF Video Protocol

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Gene Symbol IGF2R