IGF-II R/IGF2R Antibody (2G11) - BSA Free

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Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunohistochemistry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining of human testis using NB300-514 antibody at 1:100.
Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - An intracellular stain was performed on MCF7 cells with IGF-II R/IGF2R [2G11] Antibody NB300-514AF647 (blue) and a matched isotype control (orange). Cells ...read more
Immunocytochemistry/ Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei ...read more
Immunocytochemistry/ Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - HMVEC Cells
Immunocytochemistry/ Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining in Hela Cells. Mannose 6-Phosphate Receptor staining (green), F-Actin staining with ...read more
Immunocytochemistry/ Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6 ...read more
Flow Cytometry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Mannose 6 Phosphate Receptor antibody (dark blue). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
2G11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

IGF-II R/IGF2R Antibody (2G11) - BSA Free Summary

Immunogen
Purified bovine Mannose 6 Phosphate Receptor (Cation independent) [UniProt# P08169]
Epitope
In the extracellular domain of Mannose 6 Phosphate Receptor (Cation independent).
Marker
Late Endosome Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGF2R
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 1:10-1:250
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1 ug/ml
Application Notes
By Western Blot, a 300 kDa protein representing Mannose 6 Phosphate Receptor is seen in HeLa cell extract under non-reducing conditions. Immunofluorescence staining of Mannose 6 Phosphate Receptor in HeLa cells with this antibody results in perinuclear staining. This antibody is also useful in Flow Cytometry, Immunoprecipitation, ELISA and Immunohistochemistry-on paraffin embedded sections. This antibody is CyTOF ready.
Publications
Read Publications using
NB300-514 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 9119492). Does not react with Chinese hamster ovary cell Mannose 6 Phosphate Receptor (PMID: 8163521).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for IGF-II R/IGF2R Antibody (2G11) - BSA Free

  • 300 kDa mannose 6-phosphate receptor
  • cation-independent mannose-6 phosphate receptor
  • cation-independent mannose-6-phosphate receptor
  • CD222 antigen
  • CD222
  • CI Man-6-P receptor
  • CIMPR
  • CI-MPR
  • IGF2R
  • IGF-II R
  • IGF-II receptor
  • IGFIIR
  • IGF-IIR
  • insulin-like growth factor 2 receptorM6P/IGF2R
  • Insulin-like growth factor II receptor
  • M6P/IGF2 receptor
  • M6PR
  • M6P-R
  • MPR 300
  • MPR1
  • MPRI
  • MPRIM6PR

Background

Mannose 6 phosphate is a sequence tag that plays a pivotal role in delivering proteins to the lysosome. Specifically, the mannose 6 phosphate sequence is tagged to N-linked oligosaccharides of lysosomal hydrolases as they pass through the cis-Golgi network. Once the lysosomal enzymes are tagged with the M6P sequence, they are destined to the late endosome by way of vesicular transport. After a low pH dissociates the M6P Receptor from its ligand, the M6P sequences are recycled. Additionally, M6P is bound by lectin in the immune system, and is converted to fructose 6-phosphate by mannose phosphate isomerase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready

Publications for IGF-II R/IGF2R Antibody (NB300-514)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, WB.


Filter By Application
FLOW
(2)
ICC/IF
(7)
IF/IHC
(2)
IHC
(1)
IHC-Fr
(1)
WB
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB300-514 Applications Species
Calcagni' A, Staiano L, Zampelli N et al. Loss of the batten disease protein CLN3 leads to mis-trafficking of M6PR and defective autophagic-lysosomal reformation Nature communications 2023-07-03 [PMID: 37400440] (ICC/IF, FLOW) ICC/IF, FLOW
Aguilar-GonzAlez A, GonzAlez-Correa JE, Barriocanal-Casado E et al. Isogenic GAA-KO Murine Muscle Cell Lines Mimicking Severe Pompe Mutations as Preclinical Models for the Screening of Potential Gene Therapy Strategies International journal of molecular sciences 2022-06-04 [PMID: 35682977] (FLOW, Mouse) FLOW Mouse
Wang Q, Tao C, Hannan A et al. Lacrimal gland budding requires PI3K-dependent suppression of EGF signaling Science Advances 2021-07-02 [PMID: 34193412] (IHC, IHC-Fr) IHC, IHC-Fr
Muzio L, Sirtori R, Gornati D et al. Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis Nat Commun 2020-07-31 [PMID: 32737286] (IF/IHC, Mouse) IF/IHC Mouse
Kakegawa W, Katoh A, Narumi S et al. Optogenetic Control of Synaptic AMPA Receptor Endocytosis Reveals Roles of LTD in Motor Learning Neuron 2018-08-08 [PMID: 30122381] (ICC/IF, Mouse) ICC/IF Mouse
Takahashi K, Mashima H, Miura K et al. Disruption of Small GTPase Rab7 Exacerbates the Severity of Acute Pancreatitis in Experimental Mouse Models. Sci Rep. 2017-06-06 [PMID: 28588238] (IF/IHC, WB, Mouse) IF/IHC, WB Mouse
Ganley IG, Carroll K, Bittova L, Pfeffer S. Rab9 GTPase regulates late endosome size and requires effector interaction for its stability. Mol Biol Cell. 2004-12-01 [PMID: 15456905] (ICC/IF, WB, Human) ICC/IF, WB Human
Arighi CN, Hartnell LM, Aguilar RC et al. Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. J Cell Biol. 2004-04-01 [PMID: 15078903] (ICC/IF, Human) ICC/IF Human
Valdez AC, Cabaniols JP, Brown MJ, Roche PA. Syntaxin 11 is associated with SNAP-23 on late endosomes and the trans-Golgi network. J Cell Sci. 1999-03-01 [PMID: 10036234] (ICC/IF, Human) ICC/IF Human
Barker LP, George KM, Falkow S, Small PL. Differential trafficking of live and dead Mycobacterium marinum organisms in macrophages. Infect Immun. 1997-04-01 [PMID: 9119492] (ICC/IF, Mouse) ICC/IF Mouse
Show All 12 Publications.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IGF2R
Entrez
Uniprot