Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunohistochemistry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining of human testis using NB300-514 antibody at 1:100.
Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - An intracellular stain was performed on MCF7 cells with IGF-II R/IGF2R [2G11] Antibody NB300-514AF647 (blue) and a matched isotype control (orange). Cells ...read more
Immunocytochemistry/ Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei ...read more
By Western Blot, a 300 kDa protein representing Mannose 6 Phosphate Receptor is seen in HeLa cell extract under non-reducing conditions. Immunofluorescence staining of Mannose 6 Phosphate Receptor in HeLa cells with this antibody results in perinuclear staining. This antibody is also useful in Flow Cytometry, Immunoprecipitation, ELISA and Immunohistochemistry-on paraffin embedded sections. This antibody is CyTOF ready.
Mouse reactivity reported in scientific literature (PMID: 9119492). Does not react with Chinese hamster ovary cell Mannose 6 Phosphate Receptor (PMID: 8163521).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein A purified
Alternate Names for IGF-II R/IGF2R Antibody (2G11)
300 kDa mannose 6-phosphate receptor
cation-independent mannose-6 phosphate receptor
cation-independent mannose-6-phosphate receptor
CI Man-6-P receptor
insulin-like growth factor 2 receptorM6P/IGF2R
Insulin-like growth factor II receptor
Mannose 6 phosphate is a sequence tag that plays a pivotal role in delivering proteins to the lysosome. Specifically, the mannose 6 phosphate sequence is tagged to N-linked oligosaccharides of lysosomal hydrolases as they pass through the cis-Golgi network. Once the lysosomal enzymes are tagged with the M6P sequence, they are destined to the late endosome by way of vesicular transport. After a low pH dissociates the M6P Receptor from its ligand, the M6P sequences are recycled. Additionally, M6P is bound by lectin in the immune system, and is converted to fructose 6-phosphate by mannose phosphate isomerase.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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