IGF-II R/IGF2R Antibody (2G11)

Images

 
Immunocytochemistry/ Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6 ...read more
Immunohistochemistry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining of human testis using NB300-514 antibody at 1:100.
Flow Cytometry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Mannose 6 Phosphate Receptor antibody (dark blue). ...read more
Immunocytochemistry/ Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei ...read more
Immunocytochemistry/ Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - HMVEC Cells
Immunocytochemistry/ Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining in Hela Cells. Mannose 6-Phosphate Receptor staining (green), F-Actin staining with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
2G11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

IGF-II R/IGF2R Antibody (2G11) Summary

Immunogen
Purified bovine Mannose 6 Phosphate Receptor (Cation independent) [UniProt# P08169]
Epitope
In the extracellular domain of Mannose 6 Phosphate Receptor (Cation independent).
Marker
Late Endosome Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGF2R
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:250
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
By Western Blot, a 300 kDa protein representing Mannose 6 Phosphate Receptor is seen in HeLa cell extract under non-reducing conditions. Immunofluorescence staining of Mannose 6 Phosphate Receptor in HeLa cells with this antibody results in perinuclear staining. This antibody is also useful in Flow Cytometry, Immunoprecipitation, ELISA and Immunohistochemistry-on paraffin embedded sections. This antibody is CyTOF ready.
Publications
Read Publications using
NB300-514 in the following applications:

  • 6 publications
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 9119492). Does not react with Chinese hamster ovary cell Mannose 6 Phosphate Receptor.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for IGF-II R/IGF2R Antibody (2G11)

  • 300 kDa mannose 6-phosphate receptor
  • cation-independent mannose-6 phosphate receptor
  • cation-independent mannose-6-phosphate receptor
  • CD222 antigen
  • CD222
  • CI Man-6-P receptor
  • CIMPR
  • CI-MPR
  • IGF2R
  • IGF-II R
  • IGF-II receptor
  • IGFIIR
  • IGF-IIR
  • insulin-like growth factor 2 receptorM6P/IGF2R
  • Insulin-like growth factor II receptor
  • M6P/IGF2 receptor
  • M6PR
  • M6P-R
  • MPR 300
  • MPR1
  • MPRI
  • MPRIM6PR

Background

Mannose 6 phosphate is a sequence tag that plays a pivotal role in delivering proteins to the lysosome. Specifically, the mannose 6 phosphate sequence is tagged to N-linked oligosaccharides of lysosomal hydrolases as they pass through the cis-Golgi network. Once the lysosomal enzymes are tagged with the M6P sequence, they are destined to the late endosome by way of vesicular transport. After a low pH dissociates the M6P Receptor from its ligand, the M6P sequences are recycled. Additionally, M6P is bound by lectin in the immune system, and is converted to fructose 6-phosphate by mannose phosphate isomerase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB391
Species: Hu, Mu
Applications: WB, Flow, IHC, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P
NBP2-67726
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
AF1029
Species: Mu
Applications: WB, IHC, IP, ICC
NBP2-20439
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-87299
Species: Hu
Applications: WB, ICC/IF
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
NBP1-28566
Species: Hu, Mu, Rt, Bv, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
NBP1-89917
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83220
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB300-514
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready

Publications for IGF-II R/IGF2R Antibody (NB300-514)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(6)
IHC
(1)
WB
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB300-514 Applications Species
Kakegawa W, Katoh A, Narumi S et al. Optogenetic Control of Synaptic AMPA Receptor Endocytosis Reveals Roles of LTD in Motor Learning Neuron Aug 8 2018 [PMID: 30122381] (ICC/IF, Mouse) ICC/IF Mouse
Takahashi K, Mashima H, Miura K et al. Disruption of Small GTPase Rab7 Exacerbates the Severity of Acute Pancreatitis in Experimental Mouse Models. Sci Rep. 2017 Jun 06 [PMID: 28588238] (WB, IHC, Mouse) WB, IHC Mouse
Ganley IG, Carroll K, Bittova L, Pfeffer S. Rab9 GTPase regulates late endosome size and requires effector interaction for its stability. Mol Biol Cell. 2004 Dec [PMID: 15456905] (ICC/IF, WB, Human) ICC/IF, WB Human
Arighi CN, Hartnell LM, Aguilar RC et al. Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. J Cell Biol. 2004 Apr [PMID: 15078903] (ICC/IF, Human) ICC/IF Human
Valdez AC, Cabaniols JP, Brown MJ, Roche PA. Syntaxin 11 is associated with SNAP-23 on late endosomes and the trans-Golgi network. J Cell Sci. 1999 Mar [PMID: 10036234] (ICC/IF, Human) ICC/IF Human
Barker LP, George KM, Falkow S, Small PL. Differential trafficking of live and dead Mycobacterium marinum organisms in macrophages. Infect Immun. 1997 Apr [PMID: 9119492] (ICC/IF, Mouse) ICC/IF Mouse
Heinzen RA, Scidmore MA, Rockey DD, Hackstadt T. Differential interaction with endocytic and exocytic pathways distinguish parasitophorous vacuoles of Coxiella burnetii and Chlamydia trachomatis. Infect Immun. 1996 Mar [PMID: 8641784] (ICC/IF, Human) ICC/IF Human
Dintzis SM et al. Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor. J Biol Chem 269:12159-66. 1994 [PMID: 8163521]

Reviews for IGF-II R/IGF2R Antibody (NB300-514) (0)

There are no reviews for IGF-II R/IGF2R Antibody (NB300-514). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IGF-II R/IGF2R Antibody (NB300-514) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-514AF405
Alexa Fluor 488 Labeled NB300-514AF488
Alexa Fluor 647 Labeled NB300-514AF647
Alexa Fluor 700 Labeled NB300-514AF700
Allophycocyanin Labeled NB300-514APC
DyLight 350 Labeled NB300-514UV
DyLight 405 Labeled NB300-514V
DyLight 488 Labeled NB300-514G
DyLight 680 Labeled NB300-514FR
DyLight 755 Labeled NB300-514IR
PE Labeled NB300-514PE

Additional Array Products

Bioinformatics Tool for IGF-II R/IGF2R Antibody (NB300-514)

Discover related pathways, diseases and genes to IGF-II R/IGF2R Antibody (NB300-514). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IGF-II R/IGF2R Antibody (NB300-514)

Discover more about diseases related to IGF-II R/IGF2R Antibody (NB300-514).
 

Pathways for IGF-II R/IGF2R Antibody (NB300-514)

View related products by pathway.

PTMs for IGF-II R/IGF2R Antibody (NB300-514)

Learn more about PTMs related to IGF-II R/IGF2R Antibody (NB300-514).
 

Research Areas for IGF-II R/IGF2R Antibody (NB300-514)

Find related products by research area.

Blogs on IGF-II R/IGF2R

There are no specific blogs for IGF-II R/IGF2R, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IGF-II R/IGF2R Antibody (2G11) and receive a gift card or discount.

Bioinformatics

Gene Symbol IGF2R
Entrez
Uniprot