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HAF007). A specific band was detected for IGF-I R/IGF1R at approximately 275 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox" target="_blank">
MAB002, open histogram) followed by anti-mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox" target="_blank">
NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane in MCF-7 cell line. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank">
VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by rhIGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391). At 11 µg/mL, this antibody will neutralize 50-75% rhIGF-1 induced activity." class="big_lightbox" target="_blank">
391-GR) was serially diluted and captured by Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">
291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA). The ND50 is typically 0.5-1.5 µg/mL." class="big_lightbox" target="_blank">
391-GR) was serially diluted and captured by Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival
![Western Blot IGF-I R/IGF1R Antibody (33255) [Unconjugated]](http://images.novusbio.com/images/antibody/IGF-I_R_MAB391_Western_Blot_6136.jpg)
![Immunocytochemistry IGF-I R/IGF1R Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IGFI_R_AF305NA_Immunocytochemistry__Immunofluorescence_21673.jpg)
![N/A IGF-I R/IGF1R [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IGFI_R_DY391_ELISA_1989.jpg)
![ELISA IGF-I R/IGF1R [Biotin]](http://images.novusbio.com/fullsize/dyc305-2_human-total-igf-i-r-duoset-ic-elisa-2-plate-elisa-169202094215.jpg)
![SEC-MALS IGF-I R/IGF1R [Unconjugated]](http://images.novusbio.com/fullsize/305-gr_recombinant-human-igf-i-r-igf1r-his-tagged-protein-cf-sec-mals-2322024173336.jpg)
![Flow Cytometry IGF-I R/IGF1R Antibody (33255) [Unconjugated]](http://images.novusbio.com/images/antibody/IGFI_R_MAB391_Flow_Cytometry_22536.jpg)
![Immunocytochemistry IGF-I R/IGF1R Antibody (33255) [Unconjugated]](http://images.novusbio.com/images/antibody/IGFI_R_MAB391_Immunocytochemistry__Immunofluorescence_22795.jpg)
![Immunohistochemistry IGF-I R/IGF1R Antibody (33255) [Unconjugated]](http://images.novusbio.com/images/antibody/IGFI_R_MAB391_Immunohistochemistry_21743.jpg)
![Neutralization IGF-I R/IGF1R Antibody (33255) [Unconjugated]](http://images.novusbio.com/images/antibody/IGF-I_R_MAB391_Block_Neutralize_9173.jpg)
![Immunocytochemistry/ Immunofluorescence IGF-I R/IGF1R Antibody (33255) [Unconjugated]](http://images.novusbio.com/fullsize/mab391_human-mouse-igf-i-r-mab-clone-33255-812025554172.jpg)
![ELISA IGF-I R/IGF1R Antibody (33255) [Unconjugated]](http://images.novusbio.com/images/mab391_human-mouse-igf-i-r-igf1r-mab-clone-33255-elisa-639201778206481836.jpg)
![Immunohistochemistry IGF-I R/IGF1R Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IGF-I_R_AF-305-NA_Immunohistochemistry_10243.jpg)
![Immunohistochemistry IGF-I R/IGF1R Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IGF-I_R_AF-305-NA_Immunohistochemistry_6660.jpg)
![Neutralization IGF-I R/IGF1R Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IGF-I_R_AF-305-NA_Block_Neutralize_8690.jpg)
![Immunohistochemistry IGF-I R/IGF1R Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-305-na_human-mouse-igf-i-r-affinity-purified-polyclonal-ab-8220249511435.jpg)
![Immunohistochemistry IGF-I R/IGF1R Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-305-na_human-mouse-igf-i-r-affinity-purified-polyclonal-ab-8220249504639.jpg)
![Immunohistochemistry IGF-I R/IGF1R Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-305-na_human-mouse-igf-i-r-affinity-purified-polyclonal-ab-8220249505953.jpg)
![Immunohistochemistry IGF-I R/IGF1R Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-305-na_human-mouse-igf-i-r-affinity-purified-polyclonal-ab-8220249503241.jpg)
![Immunohistochemistry IGF-I R/IGF1R Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-305-na_human-mouse-igf-i-r-affinity-purified-polyclonal-ab-4420241542506.jpg)
![ELISA IGF-I R/IGF1R Antibody [Unconjugated]](http://images.novusbio.com/images/af-305-na_human-mouse-igf-i-r-igf1r-affinity-purified-polyclonal-ab-elisa-639201852917205759.jpg)
