IFN-gamma Antibody [Biotin]

• Reactivity: Fe
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IFN-gamma Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant feline IFN-gamma
  Gln24-Lys167
  Accession # P46402
• Specificity: Detects feline IFN-gamma in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human IFN-gamma , recombinant mouse IFN-gamma , and recombinant rat IFN-gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma Antibody [Biotin]

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature feline IFN-gamma exists as a noncovalently linked homodimer of 20  25 kDa variably glycosylated subunits (3, 4). It shares 88% amino acid sequence identity with canine IFN-gamma , 72%‑78% with bovine, equine, and porcine IFN‑ gamma , and 40%‑62% with cotton rat, human, mouse, rat, and rhesus IFN-gamma . IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (5, 6). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (7). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (7, 8). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (9, 10). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (8).

1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
3. Argyle, D.J. et al. (1995) DNA Seq. 5:169.
4. Schijns, V.E. et al. (1995) Immunogenetics 42:440.
5. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
6. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
7. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
8. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
9. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
10. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma Antibody (BAF764)(2)

Publications using BAF764

• Schlecht-Louf G, Mangeney M, El-Garch H, Lacombe V, Poulet H, Heidmann T A targeted mutation within the feline leukemia virus (FeLV) envelope protein immunosuppressive domain to improve a canarypox virus-vectored FeLV vaccine. J Virol, 2013-11-06;88(2):992-1001. 2013-11-06 [PMID: 24198407] (ELISA Development, Feline)
• Cupillard L, Juillard V, Latour S, Colombet G, Cachet N, Richard S, Blanchard S, Fischer L Impact of plasmid supercoiling on the efficacy of a rabies DNA vaccine to protect cats. Vaccine, 2005-03-14;23(16):1910-6. 2005-03-14 [PMID: 15734063] (ELISpot Development, Feline)

FAQs for IFN-gamma Antibody (BAF764). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG
• Uniprot:
  • P46402()