![Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) [NBP2-53332] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)](http://images.novusbio.com/fullsize2/IFN-gamma-Antibody-(IFNG-466)-Immunohistochemistry-Paraffin-NBP2-53332-img0003.jpg "Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) [NBP2-53332] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IHC-P |
| Clone | IFNG/466 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | Purified recombinant human IFN-gamma |
| Localization | Cytoplasmic & Cell Surface |
| Protein/Peptide Type | Recombinant Protein |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IFNG |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-P:Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes |
| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![IL-6 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-54394AF350 | |
| Alexa Fluor 405 | NBP2-54394AF405 | |
| Alexa Fluor 488 | NBP2-54394AF488 | |
| Alexa Fluor 532 | NBP2-54394AF532 | |
| Alexa Fluor 594 | NBP2-54394AF594 | |
| Alexa Fluor 647 | NBP2-54394AF647 | |
| Alexa Fluor 700 | NBP2-54394AF700 | |
| Alexa Fluor 750 | NBP2-54394AF750 | |
| Allophycocyanin | NBP2-54394APC | |
| Allophycocyanin/Cy7 | NBP2-54394APCCY7 | |
| Biotin | NBP2-54394B | |
| DyLight 350 | NBP2-54394UV | |
| DyLight 405 | NBP2-54394V | |
| DyLight 488 | NBP2-54394G | |
| DyLight 550 | NBP2-54394R | |
| DyLight 594 | NBP2-54394DL594 | |
| DyLight 650 | NBP2-54394C | |
| DyLight 680 | NBP2-54394FR | |
| DyLight 755 | NBP2-54394IR | |
| FITC | NBP2-54394F | |
| HRP | NBP2-54394H | |
| Janelia Fluor 549 | NBP2-54394JF549 | |
| Janelia Fluor 646 | NBP2-54394JF646 | |
| PE | NBP2-54394PE | |
| PE/Atto594 | NBP2-54394PEATT594 | |
| PE/Cy5.5 | NBP2-54394PECY55 | |
| PE/Cy7 | NBP2-54394PECY7 | |
| PerCP | NBP2-54394PCP |
Diseases for IFN-gamma Antibody (NBP2-53332)Discover more about diseases related to IFN-gamma Antibody (NBP2-53332).
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PTMs for IFN-gamma Antibody (NBP2-53332)Learn more about PTMs related to IFN-gamma Antibody (NBP2-53332).
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Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers... Read full blog post. |
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You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t... Read full blog post. |
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iNOS: The Nitric Oxide Boss Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti... Read full blog post. |
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| Gene Symbol | IFNG |
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) [NBP2-53332] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)](http://images.novusbio.com/fullsize2/IFN-gamma-Antibody-(IFNG-466)-Immunohistochemistry-Paraffin-NBP2-53332-img0001.jpg "Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) [NBP2-53332] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)")
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) [NBP2-53332] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)](http://images.novusbio.com/fullsize2/IFN-gamma-Antibody-(IFNG-466)-Immunohistochemistry-Paraffin-NBP2-53332-img0002.jpg "Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) [NBP2-53332] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)")
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/fullsize2/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize2/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19371-img0005.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.](http://images.novusbio.com/fullsize2/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.](http://images.novusbio.com/fullsize2/CD8-alpha-Antibody-(53-6.7)-Immunocytochemistry-NBP1-49045-img0011.jpg)
![Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.](http://images.novusbio.com/fullsize2/CD8-alpha-Antibody-(53-6.7)-Immunohistochemistry-Frozen-NBP1-49045-img0015.jpg)
![Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.](http://images.novusbio.com/fullsize2/CD8-alpha-Antibody-(53-6.7)-Flow-Cytometry-NBP1-49045-img0014.jpg)
![Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.](http://images.novusbio.com/fullsize2/IFN-alpha-1-Antibody-Western-Blot-NBP2-75930-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/IFN-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75930-img0004.jpg)
![Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/IFN-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-75930-img0007.jpg)
![Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1ug/mL for 16 hours (right lane) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize2/iNOS-Antibody-Western-Blot-NB300-605-img0015.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.](http://images.novusbio.com/fullsize2/iNOS-Antibody-Immunohistochemistry-Paraffin-NB300-605-img0010.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.](http://images.novusbio.com/fullsize2/iNOS-Antibody-Immunohistochemistry-Paraffin-NB300-605-img0016.jpg)
![Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R).](http://images.novusbio.com/fullsize2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Western-Blot-NBP2-79843-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red).](http://images.novusbio.com/fullsize2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79843-img0002.jpg)
![Immunohistochemistry-Paraffin: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DQ/DR/DP Antibody (HLA-Pan/2967R).](http://images.novusbio.com/fullsize2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79843-img0005.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Flow-(Intracellular)-NB100-524-img0010.jpg)
