ICAM-1/CD54 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

ICAM-1/CD54 Antibody [Biotin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1
  Extracellular domain
• Specificity: Detects human ICAM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with rhICAM-2 and rhICAM-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ICAM1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human ICAM-1/CD54 Antibody (Catalog # MAB720)
  ELISA Detection: Human ICAM-1/CD54 Biotinylated Antibody (Catalog # BAF720)
  Standard: Recombinant Human ICAM-1/CD54 (Catalog # ADP4)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody [Biotin]

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICAM-1/CD54 Antibody (BAF720)(5)

Publications using BAF720

• Adukpo , Selorme, Kusi , Kwadwo A, Ofori , Michael, Tetteh , John K A, Amoako-Sakyi , Daniel, Goka , Bamenla, Adjei , George O, Edoh , Dominic, Akanmori , Bartholo, Gyan , Ben A, Dodoo , Daniel High plasma levels of soluble intercellular adhesion molecule (ICAM)-1 are associated with cerebral malaria. PLoS ONE, 2013-12-27;8(12):e84181. 2013-12-27 [PMID: 24386348] (ELISA Development, Human)
• Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques. Thromb. Haemost., 2011-11-10;107(1):140-9. 2011-11-10 [PMID: 22072012] (ELISA Development, Human)
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)
• Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007-10-22;329(1):214-8. 2007-10-22 [PMID: 17964594] (Luminex Development, Human)
• Groot F, van Capel TM, Kapsenberg ML, Berkhout B, de Jong EC Opposing roles of blood myeloid and plasmacytoid dendritic cells in HIV-1 infection of T cells: transmission facilitation versus replication inhibition. Blood, 2006-05-16;108(6):1957-64. 2006-05-16 [PMID: 16705088] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ICAM1