IBTK Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: IBTK Antibody [NBP1-88512] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Simple Western: IBTK Antibody [NBP1-88512] - Simple Western lane view shows a specific band for IBTK in 0.2 mg/ml of U-251 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: IBTK Antibody [NBP1-88512] - Electropherogram image(s) of corresponding Simple Western lane view. IBTK antibody was used at 1:20 dilution on U-251 lysate(s).

Flow Cytometry: IBTK Antibody [NBP1-88512] - IBTK alpha RNA interference increases spontaneous & fludarabine–induced apoptosis in MEC-1 CLL cells MEC-1 cells were transduced with TurboGFP-shCTRL or TurboGFP-shIBTK alpha (Sigma-Aldrich), & after 5 days were left untreated (N.T.) a, b or 72 h-treated with Fludarabine at 25 µM c & d or 50 µM e, f. Then, cells were collected, stained with Annexin-V-APC & analysed by flow cytometry. Binding of Annexin-V-APC was measured for GFP-positive cells, as positive control of transduction. A representative experiment of three independent experiments is shown. Statistically significant differences were assessed using Mann–Whitney test (GraphPad Prism 6), as follows: a vs. bp = 0.0043; c vs. dp = 0.0002; e vs. fp = 0.0001 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-017-0026-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IBTK Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IBTK Antibody - BSA Free (NBP1-88512) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western and IP. Anti-IBTK Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LKEICEVALTEKLTLKNAAMLLEFAAMYSAKQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IBTK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation Reported in scientific literature (PMID: 28710282).
  • Simple Western 1:20
  • Western Blot Reported in scientific literature (PMID: 28710282).
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in U-251, separated by Size, antibody dilution of 1:20. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control Peptide:

  IBTK Protein (NBP1-88512PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30975981).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for IBTK Antibody - BSA Free

• Bruton agammaglobulinemia tyrosine kinase inhibitor
• BTK-binding protein
• BTKI
• DKFZP564B116
• IBtk
• inhibitor of Bruton agammaglobulinemia tyrosine kinase
• inhibitor of Bruton tyrosine kinase
• inhibitor of Bruton's tyrosine kinase
• inhibitor of Bruton's tyrosine kinase-alpha
• inhibitor of Bruton's tyrosine kinase-beta
• inhibitor of Bruton's tyrosine kinase-gamma
• KIAA1417
• MGC142256
• MGC142258
• RP1-93K22.1

Background

IBTK is encoded by this gene binds to Bruton's tyrosine kinase (BTK) and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IBTK Antibody (NBP1-88512)(3)

Publications using NBP1-88512

• Vecchio, E;Golino, G;Pisano, A;Albano, F;Falcone, C;Ceglia, S;Iaccino, E;Mimmi, S;Fiume, G;Giurato, G;Britti, D;Scala, G;Quinto, I; IBTK contributes to B-cell lymphomagenesis in E mu-myc transgenic mice conferring resistance to apoptosis Cell Death Dis 2019-04-11 [PMID: 30975981] (ICC/IF, Mouse)
• Albano F, Chiurazzi F, Mimmi S et al. The expression of inhibitor of bruton's tyrosine kinase gene is progressively up regulated in the clinical course of chronic lymphocytic leukaemia conferring resistance to apoptosis Cell Death Dis 2018-01-09 [PMID: 29317636] (ICC/IF, Human)
• Willy JA, Young SK, Mosley AL et al. Function of Inhibitor of Brutons Tyrosine Kinase Isoform alpha (IBTKalpha) in Nonalcoholic Steatohepatitis links Autophagy and the Unfolded Protein Response J. Biol. Chem. 2017-07-14 [PMID: 28710282] (WB, IP, Human)

Bioinformatics

• Gene Symbol: IBTK