Drosha Antibody

Images

 
Biological Strategies: Western Blot: Drosha Antibody [NBP1-03349] - Detection of Human Drosha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Independent Antibodies: Immunoprecipitation: Drosha Antibody [NBP1-03349] - Detection of human Drosha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Drosha Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1324 and 1374 of human double-stranded RNA-specific endoribonuclease using the numbering given in entry NP_037367.3 (GeneID 29102).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DROSHA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence -Reported in scientific literature (PMID 25351346)
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Frozen -Reported in scientific literature (PMID 25351346)
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Reviewed Applications
Read 3 Reviews rated 4
using
NBP1-03349 in the following applications:

Publications
Read Publications using
NBP1-03349 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25351346).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Drosha Antibody

  • drosha, double-stranded RNA-specific endoribonuclease
  • drosha, ribonuclease type III
  • EC 3.1.26.3
  • ETOHI2
  • nuclear RNase III Drosha
  • p241
  • Protein Drosha
  • putative protein p241 which interacts with transcription factor Sp1
  • putative ribonuclease III
  • RANSE3L
  • ribonuclease 3
  • Ribonuclease III
  • ribonuclease III, nuclear
  • RN3nuclear
  • RNase III

Background

Members of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002 [PubMed 12191433]). The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003 [PubMed 14508493]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IP

Publications for Drosha Antibody (NBP1-03349)(6)

Reviews for Drosha Antibody (NBP1-03349) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP1-03349:
Filter by Applications
IP
(1)
WB
(2)
All Applications
Filter by Species
Human
(3)
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Images Ratings Applications Species Date Details
Immunoprecipitation Drosha NBP1-03349
Enlarge
4
reviewed by:
Verified Customer
IP Human 07/06/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedA549 cells
SpeciesHuman
Lota2
Western Blot Drosha NBP1-03349
Enlarge
4
reviewed by:
Verified Customer
WB Human 07/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedA549 cells
SpeciesHuman
Lota2
  4
reviewed by:
rajesha rupaimoole
WB Human 10/06/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman ovarian cancer cell lines
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Drosha Antibody (NBP1-03349) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Drosha

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Recent Reviews

4
5
0
4
3
3
0
2
0
1
0

Verified Customer
07/06/2015
Application: IP
Species: Human

Verified Customer
07/06/2015
Application: WB
Species: Human

 
rajesha rupaimoole
10/06/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol DROSHA
Entrez
Uniprot