Biological Strategies: Western Blot: Drosha Antibody [NBP1-03349] - Detection of Human Drosha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Western Blot: Drosha Antibody [NBP1-03349] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-03349 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and ...read more
The immunogen recognized by this antibody maps to a region between residue 1324 and 1374 of human double-stranded RNA-specific endoribonuclease using the numbering given in entry NP_037367.3 (GeneID 29102).
Immunogen affinity purified
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putative protein p241 which interacts with transcription factor Sp1
putative ribonuclease III
ribonuclease III, nuclear
Members of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002 [PubMed 12191433]). The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003 [PubMed 14508493]).[supplied by OMIM]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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