MLLT1 Antibody

Western Blot: MLLT1 Antibody [NBP1-26653] - Whole cell lysate from HeLa cells. MLLT1 was also immunoprecipitated by rabbit anti-MLLT1 antibody NBP1-26652.

Immunohistochemistry-Paraffin: MLLT1 Antibody [NBP1-26653] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- MLLT1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Whole cell lysate (10 µg) from HEK293T, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLLT1 antibody used for WB at 0.04 µg/ml. Detection: Chemiluminescencewith an exposure time of 75 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MLLT1 Antibody Summary

• Description: Novus Biologicals Rabbit MLLT1 Antibody (NBP1-26653) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-MLLT1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1 using the numbering given in entry NP_005925.2 (GeneID 4298).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MLLT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MLLT1 Antibody

• ENLENL/MLL fusion
• LTG19MLLT1/MLL fusion
• myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 1
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 1
• protein ENL
• YEATS domain-containing protein 1
• YEATS1myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 1

Background

MLLT1, also known as ENL (eleven nineteen leukemia protein), was originally identified as a gene involved in a recurrent translocation (11;19) with the mixed-lineage leukemia (MLL) gene. The MLL-ENL fusion protein has been found to associate with SWI/SNF complexes and promote transcription from the HoxA7 promoter. Additionally, from studies to identify proteins that associate with MLLT1, MLLT1 is suggested to play a role in histone modification and transcriptional elongation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MLLT1 Antibody (NBP1-26653)(1)

Publication using NBP1-26653

• Dittmar G, Hernandez D P et al. PRISMA: Protein Interaction Screen on Peptide Matrix Reveals Interaction Footprints and Modifications- Dependent Interactome of Intrinsically Disordered C/EBP beta. iScience 2019-03-29 [PMID: 30884312] (IP, Human)

Bioinformatics

• Gene Symbol: MLLT1
• Entrez:
  • 4298(Human)
• Uniprot:
  • Q03111(Human)