Biological Strategies: Western Blot: Dicer Antibody [NBP1-06520] - Detection of Human and Mouse Dicer by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 ...read more
Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (50, 15 ug) from 293T cells prepared using NETN lysis buffer. Antibody: used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 ...read more
Independent Antibodies: Western Blot: Dicer Antibody [NBP1-06520] - Western Blot and Immunoprecipitation: Dicer antibody [NBP1-06520]- Whole cell lysate from 293T (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP ...read more
The immunogen recognized by this antibody maps to a region between residue 600 and 650 of human double-stranded RNA-specific endoribonuclease; dicer1 using the numbering given in entry NP_085124.2 (GeneID 23405).
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
219 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Slicing and Dicing RNA with Dicer Dicer is an RNaseIII-like enzyme capable of cleaving double-stranded RNA (dsRNA) into smaller 21-23 nt RNA fragments known as short interfering RNA (siRNAs). It targets the selective degradation of complementary RNAs in a posttranscriptional gene sile... Read full blog post.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.