Dicer Antibody

Images

 
Western Blot: Dicer Antibody [NBP1-06520] - Detection of Human and Mouse Dicer by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis ...read more
Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (50, 15 ug) from 293T cells prepared using NETN lysis buffer. Antibody: used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 ...read more
Western Blot: Dicer Antibody [NBP1-06520] - Western Blot and Immunoprecipitation: Dicer antibody [NBP1-06520]- Whole cell lysate from 293T (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Dicer Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 600 and 650 of human double-stranded RNA-specific endoribonuclease; dicer1 using the numbering given in entry NP_085124.2 (GeneID 23405).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DICER1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2- 10 ug/mg lysate
Theoretical MW
219 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Appendix Lysate (NB820-59319)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-06520 in the following applications:

Publications
Read Publications using NBP1-06520.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dicer Antibody

  • DCR1
  • dicer 1, double-stranded RNA-specific endoribonuclease
  • dicer 1, ribonuclease type III
  • Dicer
  • Dicer1, Dcr-1 homolog (Drosophila)
  • EC 3.1.26
  • EC 3.1.26.-
  • EC 3.1.26.3
  • Helicase MOI
  • Helicase with RNase motif
  • helicase-moi
  • HERNADicer1, Dcr-1 homolog
  • K12H4.8-LIKE
  • KIAA0928endoribonuclease Dicer

Background

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dicer Antibody (NBP1-06520)(3)

Review for Dicer Antibody (NBP1-06520) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-06520:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 09/13/2011
View

Summary

ApplicationWestern Blot
Sample TestedNBP1-06520, Sample Amount: 16ug
SpeciesMouse

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Dicer Antibody (NBP1-06520) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Dicer Products

Bioinformatics Tool for Dicer Antibody (NBP1-06520)

Discover related pathways, diseases and genes to Dicer Antibody (NBP1-06520). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Dicer Antibody (NBP1-06520)

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Pathways for Dicer Antibody (NBP1-06520)

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PTMs for Dicer Antibody (NBP1-06520)

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Research Areas for Dicer Antibody (NBP1-06520)

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Blogs on Dicer.

Slicing and Dicing RNA with Dicer
Dicer is an RNaseIII-like enzyme capable of cleaving double-stranded RNA (dsRNA) into smaller 21-23 nt RNA fragments known as short interfering RNA (siRNAs). It targets the selective degradation of complementary RNAs in a posttranscriptional gene sile...  Read full blog post.

Ago2 antibodies and dicer-independent biogenesis of miRNA
Ago2, also called eIF2C2, antibody is one of 37 reagents targeted to the Argonaute protein family that we at Novus Biologicals have in our antibody catalogue. Argonaute proteins are encoded by genes which play an important role in regulating the contr...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
09/13/2011
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol DICER1
Entrez
Uniprot