HYPB Antibody (CL9956)

Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.

Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Analysis in human testis and skeletal muscle tissues using NBP2-88941 antibody. Corresponding SETD2 RNA-seq data are presented for the same tissues.

• Reactivity: Hu
• Applications: IHC
• Clone: CL9956
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HYPB Antibody (CL9956) Summary

• Immunogen: This antibody was developed against a synthetic peptide corresponding to amino acids: PHHPFAGYPPGYPMQAYVDPSNPN
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SETD2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, containing 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for HYPB Antibody (CL9956)

• FLJ22472
• FLJ23184
• FLJ45883
• FLJ46217
• HIF1
• HIF-1EC 2.1.1.43
• HIP-1
• histone-lysine N-methyltransferase SETD2
• hSET2
• huntingtin interacting protein 1
• Huntingtin yeast partner B
• Huntingtin-interacting protein 1
• Huntingtin-interacting protein B
• HYPBp231HBP
• KIAA1732SET domain-containing protein 2
• KMT3AHBP231
• Lysine N-methyltransferase 3A
• SET domain containing 2
• SET2FLJ16420

Background

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SETD2