HUS1 Antibody

Images

 
Western Blot: HUS1 Antibody [NBP1-33589] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: HUS1 Antibody [NBP1-33589] - Paraffin-embedded CA922 xenograft, using antibody at 1:500 dilution.
Western Blot: HUS1 Antibody [NBP1-33589] - 30 ug C8D30 whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.71 mg/ml

Order Details

HUS1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HUS1. The exact sequence is proprietary.
Localization
Nucleus, Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HUS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.71 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HUS1 Antibody

  • checkpoint protein HUS1
  • hHUS1
  • HUS1 (S. pombe) checkpoint homolog
  • HUS1 checkpoint homolog (S. pombe)
  • hus1+-like protein

Background

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HUS1