Human Total HO-1/HMOX1 DuoSet IC ELISA, 2 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Total HO-1/HMOX1 DuoSet IC ELISA, 2 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: HMOX1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total HO-1/HMOX1 DuoSet IC ELISA, 2 Plate

• bK286B10
• EC 1.14.99.3
• heat shock protein, 32-kD
• heme oxygenase (decycling) 1
• HMOX1
• HO
• HO1
• HO-1
• HO-1heme oxygenase 1
• HSP32

Background

Heme Oxygenase (HO), also known as HMOX, is the rate limiting enzyme in the breakdown of heme into Biliverdin, CO, and iron. There are two major isoforms of this enzyme, HO-1 and HO-2. Both have similar enzymatic activity; however, HO-1 is rapidly inducible, while HO-2 is constitutively expressed. Inducers of HO-1 include cell stressors such as oxidative stress, infection, hypoxia, and cytotoxic agents. The reaction products generated by HO-1 activity are biologically active, and the enzyme has a broad range of putative roles. For instance, Biliverdin is an antioxidant with cytoprotective and anti-inflammatory properties, and CO has the potential to suppress inflammation and apoptosis. Because of these activities, HO-1 has received much attention as a therapeutic target for a range of pathological processes.

Publications for HO-1/HMOX1/HSP32 (DYC3776-2)(13)

Publications using DYC3776-2

• Kim, GO;Park, DH;Bae, JS; Protective Effects of Cirsilineol against Lipopolysaccharide-Induced Inflammation; Insights into HO-1, COX-2, and iNOS Modulation International journal of molecular sciences 2023-05-10 [PMID: 37239882] (Human)
• YT Tian, HX Sun, XH Zhou, BP Tang Long non-coding RNA DANCR alleviates acute myocardial infarction damage via regulating microRNA-509-5p/KLF transcription factor 13 pathway The Kaohsiung journal of medical sciences, 2023-04-12;0(0):. 2023-04-12 [PMID: 37042498] (Mouse)
• N Kim, C Kim, SH Ryu, GO Kim, JS Bae Anti-Inflammatory Effect of Sparstolonin B through Inhibiting Expression of NF-kappaB and STAT-1 International Journal of Molecular Sciences, 2022-09-06;23(18):. 2022-09-06 [PMID: 36142124] (Human)
• RB da Silva P, MA Mendes, VC Soares, J França-Cos, AM Sales, NC Duppré, V de Matos B, TP da Silva, PT Bozza, MT Bozza, EN Sarno, MO Moraes, GM Sperandio, RO Pinheiro Arginase 1 is a marker of protection against illness in contacts of leprosy patients Scientific Reports, 2022-05-12;12(1):7850. 2022-05-12 [PMID: 35552484] (Human)
• CL Vinhaes, RS Teixeira, JAS Monteiro-J, R Tibúrcio, JM Cubillos-A, MB Arriaga, AG Sabarin, AJ de Souza, JJ Silva, IM Lyra, AM Ladeia, BB Andrade Hydroxyurea treatment is associated with reduced degree of oxidative perturbation in children and adolescents with�sickle cell anemia Sci Rep, 2020-11-04;10(1):18982. 2020-11-04 [PMID: 33149225] (Human)
• N Cinegaglia, J Acosta-Nav, C Rainho, L Antoniazzi, S Mattioli, C Pimentel, RD Santos, V Sandrim Association of Omnivorous and Vegetarian Diets With Antioxidant Defense Mechanisms in Men J Am Heart Assoc, 2020-06-09;9(12):e015576. 2020-06-09 [PMID: 32515251] (Human)
• JR Barretto, N Boa-Sorte, CL Vinhaes, H Malta-Sant, J Rebouças-S, CF Ramos, MAS Torres-Nas, VM Borges, BB Andrade Heightened Plasma Levels of Transforming Growth Factor Beta (TGF-&beta) and Increased Degree of Systemic Biochemical Perturbation Characterizes Hepatic Steatosis in Overweight Pediatric Patients: A Cross-Sectional Study Nutrients, 2020-06-02;12(6):. 2020-06-02 [PMID: 32498337] (Human)
• CH Piao, YJ Fan, TV Nguyen, CH Song, OH Chai Mangiferin Alleviates Ovalbumin-Induced Allergic Rhinitis via Nrf2/HO-1/NF-kappaB Signaling Pathways Int J Mol Sci, 2020-05-12;21(10):. 2020-05-12 [PMID: 32408566] (Human)
• G Wang, N Mohammadtu, Y Lv, H Zhang, J Sun, J Dong Baicalin Exerts Anti-Airway Inflammation and Anti-Remodelling Effects in Severe Stage Rat Model of Chronic Obstructive Pulmonary Disease Evid Based Complement Alternat Med, 2018-10-08;2018(0):7591348. 2018-10-08 [PMID: 30402133] (Rat)
• R Budiarti, Kuntaman, Nasronudin, Suryokusum, SQ Khairunisa IN VITRO STUDIES ON HEME OXYGENASE-1 AND P24 ANTIGEN HIV-1 LEVEL AFTERHYPERBARIC OXYGEN TREATMENTOFHIV-1 INFECTED ON PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS) Afr J Infect Dis, 2018-03-07;12(1):1-6. 2018-03-07 [PMID: 29619425] (Human)
• Oyewole , Anne O, Wilmot , Marie-Cl, Fowler , Mark, Birch-Machin , Mark A Comparing the effects of mitochondrial targeted and localized antioxidants with cellular antioxidants in human skin cells exposed to UVA and hydrogen peroxide. FASEB J, 2013-10-10;28(1):485-94. 2013-10-10 [PMID: 24115050] (Human)
• Konvalinka , Ana, Zhou , Joyce, Dimitromanolakis , Apostolo, Drabovich , Andrei P, Fang , Fei, Gurley , Susan, Coffman , Thomas, John , Rohan, Zhang , Shao-Lin, Diamandis , Elefther, Scholey , James W Determination of an angiotensin II-regulated proteome in primary human kidney cells by stable isotope labeling of amino acids in cell culture (SILAC). J Biol Chem, 2013-07-11;288(34):24834-47. 2013-07-11 [PMID: 23846697] (Human)
• Blann AD, Balakrishnan B, Ryan P Increased levels of plasma haemoxygenase-1 in prostate cancer. Prostate Cancer Prostatic Dis., 2011-01-25;14(2):114-7. 2011-01-25 [PMID: 21263452] (Human)

FAQs for HO-1/HMOX1/HSP32 (DYC3776-2). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HMOX1