Adiponectin/Acrp30 Antibody [Unconjugated]

Adiponectin/Acrp30 was detected in immersion fixed ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Adiponectin/Acrp30 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Adiponectin/Acrp30 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin/Acrp30 (R&D Systems, Catalog # 5095-AC)
  Glu18-Asn247
  Accession # Q60994
• Specificity: Detects mouse Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, greater than 50% cross-reactivity with recombinant rat Adiponectin is observed and less than 30% cross-reactivity with recombinant human Adiponectin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADIPOQ

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Adiponectin/Acrp30 Antibody [Unconjugated]

• ACDC
• Acrp30
• ACRP30ADPN
• adipocyte, C1Q and collagen domain containing
• Adiponectin
• adiponectin, C1Q and collagen domain containing
• AdipoQ
• ADIPQTL1
• ApM1
• apM-1
• APM1APM-1
• C1q and collagen domain-containing protein
• GBP28
• GBP28apM1
• Gelatin-binding protein

Background

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11-13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).

1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10308.
7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
8. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
9. Hu, E. et al. (1996) J. Biol. Chem. 271:10697.
10. Waki, H. et al. (2005) Endocrinology 146:790.
11. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
12. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
13. Wang, Y. et al. (2008) Biochem. J. 409:623.
14. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
15. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adiponectin/Acrp30 Antibody (AF1119)(17)

Publications using AF1119

• DT Quiroga, MC Muñoz, C Gil, M Pffeifer, JE Toblli, UM Steckeling, JF Giani, FP Dominici Chronic administration of the angiotensin type 2 receptor agonist C21 improves insulin sensitivity in C57BL/6 mice Physiol Rep, 2018;6(16):e13824. 2018 [PMID: 30156060] (Western Blot, Mouse)
• F Sun, Y Lei, J You, C Li, L Sun, J Garza, D Zhang, M Guo, PE Scherer, D Lodge, XY Lu Adiponectin modulates ventral tegmental area dopamine neuron activity and anxiety-related behavior through AdipoR1 Mol. Psychiatry, 2018;0(0):. 2018 [PMID: 29988086] (Western Blot, Mouse)
• Y Obata, S Kita, Y Koyama, S Fukuda, H Takeda, M Takahashi, Y Fujishima, H Nagao, S Masuda, Y Tanaka, Y Nakamura, H Nishizawa, T Funahashi, B Ranscht, Y Izumi, T Bamba, E Fukusaki, R Hanayama, S Shimada, N Maeda, I Shimomura Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release JCI Insight, 2018;3(8):. 2018 [PMID: 29669945] (Mouse)
• J Shin, A Fukuhara, T Onodera, S Kita, C Yokoyama, M Otsuki, I Shimomura SDF-1 is an Autocrine Insulin-Desensitizing Factor in Adipocytes Diabetes, 2018;0(0):. 2018 [PMID: 29581126] (Western Blot, Mouse)
• C Hu, HL Keen, KT Lu, X Liu, J Wu, DR Davis, SC Ibeawuchi, S Vogel, FW Quelle, CD Sigmund Retinol-binding protein 7 is an endothelium-specific PPAR? cofactor mediating an antioxidant response through adiponectin JCI Insight, 2017;2(6):e91738. 2017 [PMID: 28352663] (IHC, Mouse)
• S Fukuda, S Kita, Y Obata, Y Fujishima, H Nagao, S Masuda, Y Tanaka, H Nishizawa, T Funahashi, J Takagi, N Maeda, I Shimomura The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2 J. Biol. Chem, 2017;0(0):. 2017 [PMID: 28325833] (Western Blot, Mouse)
• M C Muñoz Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice Peptides, 2016;0(0):. 2016 [PMID: 27979738] (Western Blot, Mouse)
• Role of the adipose PPAR?-adiponectin axis in susceptibility to stress and depression/anxiety-related behaviors Mol. Psychiatry, 2016;0(0):. 2016 [PMID: 27956741] (Western Blot, Mouse)
• Matsuda, Keisuke, Fujishima, Yuya, Maeda, Norikazu, Mori, Takuya, Hirata, Ayumu, Sekimoto, Ryohei, Tsushima, Yu, Masuda, Shigeki, Yamaoka, Masaya, Inoue, Kana, Nishizawa, Hitoshi, Kita, Shunbun, Ranscht, Barbara, Funahashi, Tohru, Shimomura, Iichiro Positive feedback regulation between adiponectin and T-cadherin impacts adiponectin levels in tissue and plasma of male mice. Endocrinology, 2015;156(3):934-46. 2015 [PMID: 25514086] (Western Blot, Mouse)
• Mori T, Koyama Y, Maeda N, Nakamura Y, Fujishima Y, Matsuda K, Funahashi T, Shimada S, Shimomura I Ultrastructural localization of adiponectin protein in vasculature of normal and atherosclerotic mice. Sci Rep, 2014;4(0):4895. 2014 [PMID: 24809933] (IHC, Mouse)
• Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013;504(7479):277-81. 2013 [PMID: 24336287] (IHC-Fr, Mouse)
• Yang J, Lin S, Chen G, He L, Hu Z, Chan L, Trial J, Entman M, Wang Y Adiponectin promotes monocyte-to-fibroblast transition in renal fibrosis. J Am Soc Nephrol, 2013;24(10):1644-59. 2013 [PMID: 23833260] (IHC, Mouse)
• Parker-Duffen, Jennifer, Nakamura, Kazuto, Silver, Marcy, Kikuchi, Ryosuke, Tigges, Ulrich, Yoshida, Sumiko, Denzel, Martin S, Ranscht, Barbara, Walsh, Kenneth T-cadherin is essential for adiponectin-mediated revascularization. J Biol Chem, 2013;288(34):24886-97. 2013 [PMID: 23824191] (Western Blot, Mouse)
• Kim H, Kwon N, Baek K, Kim D, Yun H Leucine-rich glioma inactivated 3 associates negatively with adiponectin. Cytokine, 2013;62(2):206-9. 2013 [PMID: 23548727] (Western Blot, Mouse)
• Ding M, Carrao A, Wagner R, Xie Y, Jin Y, Rzucidlo E, Yu J, Li W, Tellides G, Hwa J, Aprahamian T, Martin K Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype. J Mol Cell Cardiol, 2012;52(2):474-84. 2012 [PMID: 21952104] (ICC, Mouse)
• Wong GW, Krawczyk SA, Kitidis-Mitrokostas C, Revett T, Gimeno R, Lodish HF Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions. Biochem. J., 2008;416(2):161-77. 2008 [PMID: 18783346] (Western Blot, Human)
• Takemura Y, Ouchi N, Shibata R, Aprahamian T, Kirber MT, Summer RS, Kihara S, Walsh K Adiponectin modulates inflammatory reactions via calreticulin receptor-dependent clearance of early apoptotic bodies. J. Clin. Invest., 2007;117(2):375-86. 2007 [PMID: 17256056] (IHC-P, Mouse)

Review for Adiponectin/Acrp30 Antibody (AF1119) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23824191
• Species: Mouse

Blocking

• Blocking Details: See PMID 23824191

Details

• Detection Notes: See PMID 23824191

Comments

• Comments: Published PMID 23824191

Bioinformatics

• Gene Symbol: ADIPOQ
• Uniprot:
  • Q60994(Mouse)