Human/Mouse Total Bcl-xL DuoSet IC ELISA, 2 Plate

Lysates prepared from A431 human epithelial carcinoma cells were incubated in wells coated with Total Bcl-xL Capture Antibody. Unbound material was removed by washing and bound material was solubilized in SDS gel sample buffer. Captured proteins were electrophoresed, transferred to a PVDF membrane, and immunoblotted with Total Bcl-xL Detection Antibody. Only a single band corresponding to human Bcl-xL was detected.

A431 human epithelial carcinoma cells and NIH-3T3 mouse embryonic fibroblast cells were lysed as described in the Preparation of Samples section in the product insert (Catalog # DYC894). Human and mouse Bcl-xL were measured with this DuoSet® IC ELISA. The same lysates were immunoblotted (inset) with rat anti-mouse Bcl-xL. The DuoSet® IC ELISA results correlate with the relative amounts of Bcl-xL detected by Western Blot.

• Reactivity: Hu, Mu
• Applications: ELISA
• Conjugate: Biotin

Human/Mouse Total Bcl-xL DuoSet IC ELISA, 2 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: BCL2L1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human/Mouse Total Bcl-xL DuoSet IC ELISA, 2 Plate

• Apoptosis regulator Bcl-X
• bcl2-L-1
• BCL2-like 1
• bcl-2-like protein 1
• BCLXBCL2LBcl-X
• BclxL
• Bcl-xL
• BCL-XL/S
• BCLXS
• bcl-xS
• DKFZp781P2092

Background

Bcl-x is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Alternative splicing results in two distinct Bcl-x isoforms. Bcl-xL (long) is an anti-apoptotic protein that prevents release of Cytochromec from the mitochondrial intermembrane space into the cytosol. Bcl-xs (short) is a pro-apoptotic member that can initiate programmed cell death.

Publications for Bcl-xL (DYC894-2)(5)

Publications using DYC894-2

• Kaplan, HM;Deger, M;Erdogan, KE;Ates, T;Demir, E; Alpha-linolenic acid protects against methotrexate-induced nephrotoxicity in mouse kidney cells European review for medical and pharmacological sciences 2023-11-01 [PMID: 38039041] (Mouse)
• Kraemer B, Campbell R, Schwertz H, Franks Z, Vieira de Abreu A, Grundler K, Kile B, Dhakal B, Rondina M, Kahr W, Mulvey M, Blaylock R, Zimmerman G, Weyrich A Bacteria differentially induce degradation of Bcl-xL, a survival protein, by human platelets. Blood, 2012-10-18;120(25):5014-20. 2012-10-18 [PMID: 23086749] (Human)
• Poggi A, Prevosto C, Catellani S, Rocco I, Garuti A, Zocchi MR Engagement of CD31 delivers an activating signal that contributes to the survival of chronic lymphocytic leukaemia cells. Br. J. Haematol., 2010-08-31;151(3):252-64. 2010-08-31 [PMID: 20813004] (Human)
• Mu P, Nagahara S, Makita N, Tarumi Y, Kadomatsu K, Takei Y Systemic delivery of siRNA specific to tumor mediated by atelocollagen: combined therapy using siRNA targeting Bcl-xL and cisplatin against prostate cancer. Int. J. Cancer, 2009-12-15;125(12):2978-90. 2009-12-15 [PMID: 19422046] (Human)
• Kells AP, Connor B AAV-mediated expression of Bcl-xL or XIAP fails to induce neuronal resistance against quinolinic acid-induced striatal lesioning. Neurosci. Lett., 2008-03-22;436(3):326-30. 2008-03-22 [PMID: 18406531] (Rat)

FAQs for Bcl-xL (DYC894-2). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BCL2L1