Human Endoglin/CD105 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Endoglin/CD105 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ENG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Endoglin/CD105 DuoSet ELISA, 15 Plate

• CD105 antigen
• CD105
• Endoglin
• ENDOsler-Rendu-Weber syndrome 1
• ENG
• HHT1FLJ41744
• ORW
• ORW1

Background

Endoglin (CD105) is a transmembrane type III receptor for TGF-beta superfamily ligands and plays an important role in smooth muscle differentiation, angiogenesis, and neovascularization. It is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. Human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I. Elevated levels of anti-angiogenic soluble Endoglin contribute to pathogenicity in preeclampsia. Endoglin associates with the receptors TGF-beta RII, Activin RIIA or RIIB, BMPR-IA/ALK-3, or BMPR-IB/ALK6 and enhance binding of Activin A, BMP-2, -7, -9, TGF-beta 1, or TGF-beta 3. Endoglin can either enhance or inhibit signaling through these receptor complexes.

Publications for Endoglin/CD105 (DY1097)(19)

Publications using DY1097

• López-Guzmán, C;García, AM;Marín, P;Vásquez, AM; Assessment of the Integrity and Function of Human Term Placental Explants in Short-Term Culture Methods and protocols 2024-02-15 [PMID: 38392690] (Human)
• E Kadife, N Hannan, A Harper, N Binder, S Beard, FC Brownfoot Hydroxychloroquine reduces soluble Flt-1 secretion from human cytotrophoblast, but does not mitigate markers of endothelial dysfunction in vitro PLoS ONE, 2022-11-23;17(11):e0271560. 2022-11-23 [PMID: 36417467] (Human)
• BR Fato, N de Alwis, S Beard, NK Binder, N Pritchard, S Tong, TJ Kaitu'u-Li, NJ Hannan Serum Collected from Preeclamptic Pregnancies Drives Vasoconstriction of Human Omental Arteries-A Novel Ex Vivo Model of Preeclampsia for Therapeutic Development International Journal of Molecular Sciences, 2022-09-16;23(18):. 2022-09-16 [PMID: 36142764] (Human)
• S Guerrero, E Sánchez-Ti, L Agüí, A González-C, P Yáñez-Sede, JM Pingarrón Development of an Electrochemical CCL5 Chemokine Immunoplatform for Rapid Diagnosis of Multiple Sclerosis Biosensors, 2022-08-07;12(8):. 2022-08-07 [PMID: 36005006] (Human)
• KN Lee, KH Park, YM Kim, I Cho, TE Kim Prediction of emergency cerclage outcomes in women with cervical insufficiency: The role of inflammatory, angiogenic, and extracellular matrix-related proteins in amniotic fluid PLoS ONE, 2022-05-10;17(5):e0268291. 2022-05-10 [PMID: 35536791] (Human)
• FC Brownfoot, NJ Hannan, P Cannon, V Nguyen, R Hastie, LJ Parry, S Senadheera, L Tuohey, S Tong, TJ Kaitu'u-Li Sulfasalazine reduces placental secretion of antiangiogenic factors, up-regulates the secretion of placental growth factor and rescues endothelial dysfunction EBioMedicine, 2019-02-26;0(0):. 2019-02-26 [PMID: 30824385] (Human)
• M Varas-Godo, S Acuña-Gall, S Venegas-Du, C Hill, A Caceres-Ve, O Realini, LJ Monteiro, G Zavala, M Khoury, R Romero, G Rice, SE Illanes Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia Stem Cells Int, 2019-01-27;2019(0):1916542. 2019-01-27 [PMID: 30809262] (Human)
• NJ Hannan, NK Binder, S Beard, TV Nguyen, TJ Kaitu'u-Li, S Tong Melatonin enhances antioxidant molecules in the placenta, reduces secretion of soluble fms-like tyrosine kinase 1 (sFLT) from primary trophoblast but does not rescue endothelial dysfunction: An evaluation of its potential to treat preeclampsia PLoS ONE, 2018-04-11;13(4):e0187082. 2018-04-11 [PMID: 29641523] (Human)
• Erdman L, D'Acremont V, Hayford K, Rajwans N, Kilowoko M, Kyungu E, Hongoa P, Alamo L, Streiner D, Genton B, Kain K Biomarkers of Host Response Predict Primary End-Point Radiological Pneumonia in Tanzanian Children with Clinical Pneumonia: A Prospective Cohort Study. PLoS ONE, 2015-09-14;10(9):e0137592. 2015-09-14 [PMID: 26366571] (Human)
• McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women. PLoS ONE, 2015-08-06;10(8):e0134619. 2015-08-06 [PMID: 26247200] (Human)
• Olsen O, Wader K, Misund K, Vatsveen T, Ro T, Mylin A, Turesson I, Stordal B, Moen S, Standal T, Waage A, Sundan A, Holien T Bone morphogenetic protein-9 suppresses growth of myeloma cells by signaling through ALK2 but is inhibited by endoglin. Blood Cancer J, 2014-03-21;4(0):e196. 2014-03-21 [PMID: 24658374] (Human)
• Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study. BMC Infect Dis, 2014-01-20;14(0):35. 2014-01-20 [PMID: 24444080] (Human)
• Tong M, Han B, Holpuch A, Pei P, He L, Mallery S Inherent phenotypic plasticity facilitates progression of head and neck cancer: endotheliod characteristics enable angiogenesis and invasion. Exp Cell Res, 2013-01-29;319(7):1028-42. 2013-01-29 [PMID: 23370231] (Human)
• Munaut C, Lorquet S, Pequeux C, Coulon C, Le Goarant J, Chantraine F, Noel A, Goffin F, Tsatsaris V, Subtil D, Foidart JM Differential expression of Vegfr-2 and its soluble form in preeclampsia. PLoS ONE, 2012-03-12;7(3):e33475. 2012-03-12 [PMID: 22428059] (Human)
• Kaitu'u-Lino TJ, Whitehead CL, Ngian GL, Permezel M, Tong S Serum concentrations of soluble Flt-1 are decreased among women with a viable fetus and no symptoms of miscarriage destined for pregnancy loss. PLoS ONE, 2012-02-28;7(2):e32509. 2012-02-28 [PMID: 22389705] (Human)
• Conroy AL, Liles WC, Molyneux ME Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi. PLoS ONE, 2011-12-12;6(12):e28540. 2011-12-12 [PMID: 22174834] (Human)
• Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study. PLoS ONE, 2011-02-25;6(2):e17440. 2011-02-25 [PMID: 21364762] (Human)
• Hawinkels LJ, Kuiper P, Wiercinska E Matrix metalloproteinase-14 (MT1-MMP)-mediated endoglin shedding inhibits tumor angiogenesis. Cancer Res., 2010-04-27;70(10):4141-50. 2010-04-27 [PMID: 20424116] (Human)
• Fujita K, Ewing CM, Chan DY, Mangold LA, Partin AW, Isaacs WB, Pavlovich CP Endoglin (CD105) as a urinary and serum marker of prostate cancer. Int. J. Cancer, 2009-02-01;124(3):664-9. 2009-02-01 [PMID: 19004009] (Human)

FAQs for Endoglin/CD105 (DY1097). (Showing 1 - 3 of 3 FAQs).

1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
   • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.
3. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ENG