Human CXCL12/SDF-1 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human CXCL12/SDF-1 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CXCL12

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CXCL12/SDF-1 DuoSet ELISA, 15 Plate

• chemokine (C-X-C motif) ligand 12
• C-X-C motif chemokine 12
• CXCL12
• hIRH
• hSDF-1
• Intercrine reduced in hepatomas
• IRH
• PBSF
• PBSFSDF-1a
• Pre-B cell growth-stimulating factor
• SCYB12
• SDF1
• SDF-1
• SDF1A
• SDF1B
• SDF1SDF-1b
• stromal cell-derived factor 1
• TLSF
• TLSF-a
• TLSF-b
• TPAR1

Background

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.

Publications for CXCL12/SDF-1 (DY350)(40)

Publications using DY350

• Khan, D;Fischer, I;Sahan, S;Hewera, M;Faust, K;Muhammad, S; Platelet factor 4 and stromal cell-derived factor are novel prognostic biomarkers for cerebral vasospasm and mortality after subarachnoid hemorrhage Scientific reports 2025-09-05 [PMID: 40913116] (Human)
• Courtemanche, O;Huppé, CA;Blais-Lecours, P;Maranda, C;Morissette, MC;Blanchet, MR;Dion, G;Marsolais, D; Ex Vivo Overactivation of Lymphocyte Subsets in Fibrotic Hypersensitivity Pneumonitis Is Blunted by a Sphingosine-1-Phosphate Receptor Ligand International journal of molecular sciences 2025-03-29 [PMID: 40243992] (Human)
• Rezaei, A;Pourfarzam, S;Jamali, T;Mohammadi, NG;Andalib, A;Hassan, ZM;Ayubi, F;Ghazanfari, T; Exploring chemokines and soluble adhesion molecules in mustard lung pathogenesis International immunopharmacology 2025-02-08 [PMID: 39923581] (Human)
• Armstrong, D;Chang, CY;Hong, MJ;Green, L;Hudson, W;Shen, Y;Song, LZ;Jammi, S;Casal, B;Creighton, CJ;Carisey, A;Zhang, XH;McKenna, NJ;Kang, SW;Lee, HS;Corry, DB;Kheradmand, F; MAGE-A4-Responsive Plasma Cells Promote Non-Small Cell Lung Cancer bioRxiv : the preprint server for biology 2024-07-16 [PMID: 39071307] (Mouse)
• Lengvenyte, A;Cognasse, F;Hamzeh-Cognasse, H;Sénèque, M;Strumila, R;Olié, E;Courtet, P; Baseline circulating biomarkers, their changes, and subsequent suicidal ideation and depression severity at 6 months: A prospective analysis in patients with mood disorders Psychoneuroendocrinology 2024-07-06 [PMID: 39003840] (Human)
• Lai, TY;Chiang, TC;Lee, CY;Kuo, TC;Wu, CH;Chen, YI;Hu, CM;Maskey, M;Tang, SC;Jeng, YM;Tien, YW;Lee, EYP;Lee, WH; Unraveling the impact of cancer-associated fibroblasts on hypovascular pancreatic neuroendocrine tumors British journal of cancer 2024-02-10 [PMID: 38341509] (Human)
• Elhai, M;Micheroli, R;Houtman, M;Mirrahimi, M;Moser, L;Pauli, C;Bürki, K;Laimbacher, A;Kania, G;Klein, K;Schätzle, P;Bertoncelj, MF;Edalat, SG;Keusch, L;Khmelevskaya, A;Toitou, M;Geiss, C;Rauer, T;Sakkou, M;Kollias, G;Armaka, M;Distler, O;Ospelt, C; The long non-coding RNA HOTAIR contributes to joint-specific gene expression in rheumatoid arthritis Nature communications 2023-12-09 [PMID: 38071204] (Human)
• Juhász, L;L?rincz, H;Szentpéteri, A;Tóth, N;Varga, É;Paragh, G;Harangi, M; Decreased Serum Stromal Cell-Derived Factor-1 in Patients with Familial Hypercholesterolemia and Its Strong Correlation with Lipoprotein Subfractions International journal of molecular sciences 2023-10-18 [PMID: 37894988] (Human)
• You, S;Chen, H;Miao, M;Du, J;Che, B;Xu, T;Liu, CF;Zhang, Y;He, J;Zhong, X;Cao, Y;Zhong, C; Prognostic significance of plasma SDF-1 in acute ischemic stroke patients with diabetes mellitus: the CATIS trial Cardiovascular diabetology 2023-10-10 [PMID: 37817149] (Human)
• SK Kim, JY Choe, KY Park CXCL12 and CXCR4 as Novel Biomarkers in Uric Acid-Induced Inflammation and Patients with Gouty Arthritis Biomedicines, 2023-02-21;11(3):. 2023-02-21 [PMID: 36979628] (Human)
• W Alturaiki, H Alkadi, S Alamri, ME Awadalla, A Alfaez, A Mubarak, MA Alanazi, FQ Alenzi, BF Flanagan, B Alosaimi Association between the expression of Toll-like receptors, cytokines, and homeostatic chemokines in SARS-CoV-2 infection and COVID-19 severity Heliyon, 2022-12-24;0(0):e12653. 2022-12-24 [PMID: 36589720] (Human)
• M Ackermann, JC Kamp, C Werlein, CL Walsh, H Stark, V Prade, R Surabattul, WL Wagner, C Disney, AJ Bodey, T Illig, DJ Leeming, MA Karsdal, A Tzankov, P Boor, MP Kühnel, FP Länger, SE Verleden, HM Kvasnicka, HH Kreipe, A Haverich, SM Black, A Walch, P Tafforeau, PD Lee, MM Hoeper, T Welte, B Seeliger, S David, D Schuppan, SJ Mentzer, DD Jonigk The fatal trajectory of pulmonary COVID-19 is driven by lobular ischemia and fibrotic remodelling EBioMedicine, 2022-10-04;85(0):104296. 2022-10-04 [PMID: 36206625] (Human)
• S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis Journal of ovarian research, 2022-07-26;15(1):87. 2022-07-26 [PMID: 35883110] (Human)
• K D?bkowski, A ?ab?d?-Mas, B Do??gowska, K Safranow, M Budkowska, E Zuba-Surma, T Starzy?ska Evidence of Stem Cells Mobilization in the Blood of Patients with Pancreatitis: A Potential Link with Disease Severity Stem Cells International, 2022-07-08;2022(0):5395248. 2022-07-08 [PMID: 35846982] (Human)
• L Garcia-Ger, M García, A Garrido-Ga, S Gómez-Gonz, M Torrebadel, E Prada, G Pascual-Pa, O Muñoz, S Perez-Jaum, I Lemos, N Salvador, M Vila-Ubach, A Doncel-Req, M Suñol, AM Carcaboso, J Mora, C Lavarino MIF/CXCR4 signaling axis contributes to survival, invasion, and drug resistance of metastatic neuroblastoma cells in the bone marrow microenvironment BMC Cancer, 2022-06-17;22(1):669. 2022-06-17 [PMID: 35715791] (Human)
• D Bernareggi, Q Xie, BC Prager, J Yun, LS Cruz, TV Pham, W Kim, X Lee, M Coffey, C Zalfa, P Azmoon, H Zhu, P Tamayo, JN Rich, DS Kaufman CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity Nature Communications, 2022-04-07;13(1):1899. 2022-04-07 [PMID: 35393416] (Human)
• SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis International Journal of Molecular Sciences, 2022-01-14;23(2):. 2022-01-14 [PMID: 35055054] (Human)
• VA Guarino, A Blau, J Alvarenga, J Loscalzo, YY Zhang A Crosslinked Dextran Sulfate-Chitosan Nanoparticle for Delivery of Therapeutic Heparin-binding Proteins International journal of pharmaceutics, 2021-11-11;0(0):121287. 2021-11-11 [PMID: 34775044] (Rat)
• E Yeregui, C Viladés, P Domingo, A Ceausu, YM Pacheco, S Veloso, A Inciarte, J Vidal-Gonz, M Peraire, C Perpiñán, V Falcó, J Masip, V Alba, M Vargas, A Martí, L Reverté, J Mallolas, F Vidal, J Peraire, A Rull High circulating SDF-1and MCP-1 levels and genetic variations in CXCL12, CCL2 and CCR5: Prognostic signature of immune recovery status in treated HIV-positive patients EBioMedicine, 2020-11-06;62(0):103077. 2020-11-06 [PMID: 33166788] (Human)
• S Hässler, D Bachelet, J Duhaze, N Szely, A Gleizes, S Hacein-Bey, O Aktas, M Auer, J Avouac, M Birchler, Y Bouhnik, O Brocq, D Buck-Marti, G Cadiot, F Carbonnel, Y Chowers, M Comabella, T Derfuss, N De Vries, N Donnellan, A Doukani, M Guger, HP Hartung, E Kubala Hav, B Hemmer, T Huizinga, K Ingenhoven, PE Hyldgaard-, EC Jury, M Khalil, B Kieseier, A Laurén, R Lindberg, A Loercher, E Maggi, J Manson, C Mauri, B Mohand Oum, X Montalban, M Nachury, P Nytrova, C Richez, M Ryner, F Sellebjerg, C Sievers, D Sikkema, M Soubrier, S Tourdot, C Trang, A Vultaggio, C Warnke, S Spindeldre, P Dönnes, TP Hickling, A Hincelin M, M Allez, F Deisenhamm, A Fogdell-Ha, X Mariette, M Pallardy, P Broët, ABIRISK co Clinicogenomic factors of biotherapy immunogenicity in autoimmune disease: A prospective multicohort study of the ABIRISK consortium PLoS Med, 2020-10-30;17(10):e1003348. 2020-10-30 [PMID: 33125391] (Human)
• M Tutter, C Schug, KA Schmohl, S Urnauer, C Kitzberger, N Schwenk, M Petrini, C Zach, S Ziegler, P Bartenstei, WA Weber, G Multhoff, E Wagner, LH Lindner, PJ Nelson, C Spitzweg Regional hyperthermia enhances mesenchymal stem cell recruitment to tumor stroma: Implications for mesenchymal stem cell-based tumor therapy Mol Ther, 2020-10-15;0(0):. 2020-10-15 [PMID: 33068779] (Human)
• J Ohmes, Y Xiao, F Wang, MD Mikkelsen, TT Nguyen, H Schmidt, A Seekamp, AS Meyer, S Fuchs Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment Mar Drugs, 2020-09-21;18(9):. 2020-09-21 [PMID: 32967359] (Human)
• J O'Dwyer, M Cullen, S Fattah, R Murphy, S Stefanovic, L Kovarova, M Pravda, V Velebny, A Heise, GP Duffy, SA Cryan Development of a Sustained Release Nano-In-Gel Delivery System for the Chemotactic and Angiogenic Growth Factor Stromal-Derived Factor 1alpha Pharmaceutics, 2020-06-04;12(6):. 2020-06-04 [PMID: 32512712] (Human)
• M Colombo, S Garavelli, M Mazzola, N Platonova, D Giannandre, R Colella, L Apicella, M Lancellott, E Lesma, S Ancona, MT Palano, M Barbieri, E Taiana, E Lazzari, A Basile, M Turrini, A Pistocchi, A Neri, R Chiaramont Multiple myeloma exploits Jagged1 and Jagged2 to promote intrinsic and bone marrow-dependent drug resistance Haematologica, 2019-10-03;0(0):. 2019-10-03 [PMID: 31582544] (Human)
• M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer Cancers (Basel), 2019-05-13;11(5):. 2019-05-13 [PMID: 31086083] (Human)
• U Hopfner, MM Aitzetmuel, P Ne beta bach, MS Hu, HG Machens, ZN Maan, D Duscher Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing Stem Cells Int, 2018-12-19;2018(0):1353085. 2018-12-19 [PMID: 30662467] (Human)
• F Mizoguchi, K Slowikowsk, K Wei, JL Marshall, DA Rao, SK Chang, HN Nguyen, EH Noss, JD Turner, BE Earp, PE Blazar, J Wright, BP Simmons, LT Donlin, GD Kalliolias, SM Goodman, VP Bykerk, LB Ivashkiv, JA Lederer, N Hacohen, PA Nigrovic, A Filer, CD Buckley, S Raychaudhu, MB Brenner Functionally distinct disease-associated fibroblast subsets in rheumatoid arthritis Nat Commun, 2018-02-23;9(1):789. 2018-02-23 [PMID: 29476097] (Human)
• CS Marques, M Soares, A Santos, J Correia, F Ferreira Serum SDF-1 levels are a reliable diagnostic marker of feline mammary carcinoma, discriminating HER2-overexpressing tumors from other subtypes Oncotarget, 2017-11-11;8(62):105775-105789. 2017-11-11 [PMID: 29285291] (Feline)
• BW Megra, EA Eugenin, JW Berman The Role of Shed PrP(c) in the Neuropathogenesis of HIV Infection J. Immunol., 2017-05-22;0(0):. 2017-05-22 [PMID: 28533442] (Human)
• Gabriela Odent Grig Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells J Tissue Eng Regen Med, 2016-12-12;0(0):. 2016-12-12 [PMID: 27943613] (Human)
• Marco Santoro MODELING STROMA-INDUCED DRUG RESISTANCE IN A TISSUE-ENGINEERED TUMOR MODEL OF EWING SARCOMA Tissue Eng Part A, 2017-01-01;0(0):. 2017-01-01 [PMID: 27923328] (Human)
• Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles Int J Nanomedicine, 2016-11-18;11(0):6149-6159. 2016-11-18 [PMID: 27920522] (Rat)
• Calcaterra F, Taddeo A, Colombo E, Cappelletti M, Martinelli A, Calabrese S, Mavilio D, Cetin I, Della Bella S Reduction of maternal circulating endothelial progenitor cells in human pregnancies with intrauterine growth restriction. Placenta, 2014-04-26;35(7):431-6. 2014-04-26 [PMID: 24819155] (Human)
• Austin W. Boesch, Yifeng Zhao, Alison S. Landman, Marta Rodriguez Garcia, John V. Fahey, Charles R. Wira et al. A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions Journal of Immunological Methods 2013-11-29 [PMID: 24035708] (Human)
• CXCR4 positive and angiogenic monocytes in myocardial infarction. Thromb Haemost, 2012-12-06;109(2):255-62. 2012-12-06 [PMID: 23223950] (Human)
• Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Clin. Cancer Res., 2011-04-11;17(11):3619-30. 2011-04-11 [PMID: 21482691] (Human)
• Zimmer A, Luce S, Gaignier F, Nony E, Naveau M, Biola-Vidamment A, Pallardy M, Van Overtvelt L, Mascarell L, Moingeon P Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae. J. Immunol., 2011-03-02;186(7):3966-76. 2011-03-02 [PMID: 21368225] (Human)
• Chatterjee M, Huang Z, Zhang W, Jiang L, Hultenby K, Zhu L, Hu H, Nilsson GP, Li N Distinct platelet packaging, release, and surface expression of proangiogenic and antiangiogenic factors on different platelet stimuli. Blood, 2011-02-17;117(14):3907-11. 2011-02-17 [PMID: 21330475] (Human)
• Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis. Eur. J. Immunol., 2008-11-01;38(11):3050-9. 2008-11-01 [PMID: 18924211] (Human)
• Jung Y, Wang J, Schneider A, Sun YX, Koh-Paige AJ, Osman NI, McCauley LK, Taichman RS Regulation of SDF-1 (CXCL12) production by osteoblasts; a possible mechanism for stem cell homing. Bone, 2005-12-05;38(4):497-508. 2005-12-05 [PMID: 16337237] (Human)

FAQs for CXCL12/SDF-1 (DY350). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CXCL12