Human Carbonic Anhydrase IX Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Carbonic Anhydrase IX Quantikine ELISA Kit Summary

• Background: The Quantikine Human Carbonic Anhydrase IX Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CA9 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CA9 ectodomain and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CA9 showed linear curves that were parallel to the standard... curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CA9.
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• Specificity: Natural and recombinant human CA9
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CA9

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Carbonic Anhydrase IX Quantikine ELISA Kit

• CA9
• CAIX
• CA-IX
• Carbonate dehydratase IX
• carbonic anhydrase 9
• Carbonic Anhydrase IX
• carbonic dehydratase
• EC 4.2.1.1
• G250
• Membrane antigen MN
• MN
• P54/58N
• PMW1
• RCC
• RCC-associated antigen G250
• RCC-associated protein G250
• Renal cell carcinoma-associated antigen G250

Background

Carbonic anhydrases catalyze the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Human members of the CAs are secreted (CA6), membrane-bound (CA4, 9 to 12 and 14), cytosolic (CA1 to 3, 7, 8 and 13) or mitochondrial (CA5A and 5B). Some CAs may serve as markers for tumors and hypoxia. A subset of CAs lack CA activity due to point mutations but retain esterase function.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Carbonic Anhydrase IX/CA9 (DCA900)(10)

Publications using DCA900

• N Oishi, T Hundal, JL Phillips, S Dasari, G Hu, DS Viswanatha, R He, M Mai, HK Jacobs, NH Ahmed, SI Syrbu, Y Salama, JR Chapman, F Vega, J Sidhu, NN Bennani, AL Epstein, LJ Medeiros, MW Clemens, RN Miranda, AL Feldman Molecular profiling reveals a hypoxia signature in breast implant-associated anaplastic large cell lymphoma Haematologica, 2021-06-01;0(0):. 2021-06-01 [PMID: 32414854] (Human)
• L Klameth, B Rath, M Hochmaier, D Moser, M Redl, F Mungenast, K Gelles, E Ulsperger, R Zeillinger, G Hamilton Small cell lung cancer: model of circulating tumor cell tumorospheres in chemoresistance Sci Rep, 2017-07-13;7(1):5337. 2017-07-13 [PMID: 28706293] (Human)
• DNA-linked Inhibitor Antibody Assay (DIANA) for sensitive and selective enzyme detection and inhibitor screening Nucleic Acids Res, 2016-09-26;0(0):. 2016-09-26 [PMID: 27679479] (Human)
• Shimizu Y, Furuya H, Bryant Greenwood P, Chan O, Dai Y, Thornquist M, Goodison S, Rosser C A multiplex immunoassay for the non-invasive detection of bladder cancer. J Transl Med, 2016-01-30;14(1):31. 2016-01-30 [PMID: 26830497] (Human)
• Goodison , Steve, Chang , Myron, Dai , Yunfeng, Urquidi , Virginia, Rosser , Charles A multi-analyte assay for the non-invasive detection of bladder cancer. PLoS ONE, 2012-10-19;7(10):e47469. 2012-10-19 [PMID: 23094052] (Human)
• Prognostic utility of pre-operative circulating osteopontin, carbonic anhydrase IX and CRP in renal cell carcinoma. Br. J. Cancer, 2012-08-23;107(7):1131-7. 2012-08-23 [PMID: 22918393] (Human)
• de Groot JF, Piao Y, Tran H, Gilbert M, Wu HK, Liu J, Bekele BN, Cloughesy T, Mehta M, Robins HI, Lassman A, Deangelis L, Camphausen K, Chen A, Yung W, Prados M, Wen PY, Heymach JV Myeloid Biomarkers Associated with Glioblastoma Response to Anti-VEGF Therapy with Aflibercept. Clin. Cancer Res., 2011-06-01;17(14):4872-4881. 2011-06-01 [PMID: 21632852] (Human)
• Wind TC, Messenger MP, Thompson D, Selby PJ, Banks RE Measuring carbonic anhydrase IX as a hypoxia biomarker: differences in concentrations in serum and plasma using a commercial enzyme-linked immunosorbent assay due to influences of metal ions. Ann. Clin. Biochem., 2011-03-02;48(0):112-20. 2011-03-02 [PMID: 21367885] (Human)
• Ilie M, Mazure NM, Hofman V, Ammadi RE, Ortholan C, Bonnetaud C, Havet K, Venissac N, Mograbi B, Mouroux J, Pouyssegur J, Hofman P High levels of carbonic anhydrase IX in tumour tissue and plasma are biomarkers of poor prognostic in patients with non-small cell lung cancer. Br. J. Cancer, 2010-05-11;102(11):1627-35. 2010-05-11 [PMID: 20461082] (Human)
• Tanaka N, Kato H, Inose T, Kimura H, Faried A, Sohda M, Nakajima M, Fukai Y, Miyazaki T, Masuda N, Fukuchi M, Kuwano H Expression of carbonic anhydrase 9, a potential intrinsic marker of hypoxia, is associated with poor prognosis in oesophageal squamous cell carcinoma. Br. J. Cancer, 2008-10-07;99(9):1468-75. 2008-10-07 [PMID: 18841153] (Human)

FAQs for Carbonic Anhydrase IX/CA9 (DCA900). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CA9