Human Carbonic Anhydrase IX DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Carbonic Anhydrase IX DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CA9

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Carbonic Anhydrase IX DuoSet ELISA, 15 Plate

• CA9
• CAIX
• CA-IX
• Carbonate dehydratase IX
• carbonic anhydrase 9
• Carbonic Anhydrase IX
• carbonic dehydratase
• EC 4.2.1.1
• G250
• Membrane antigen MN
• MN
• P54/58N
• PMW1
• RCC
• RCC-associated antigen G250
• RCC-associated protein G250
• Renal cell carcinoma-associated antigen G250

Background

Carbonic anhydrases catalyze the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Human members of the CAs are secreted (CA6), membrane-bound (CA4, 9 to 12 and 14), cytosolic (CA1 to 3, 7, 8 and 13) or mitochondrial (CA5A and 5B). Some CAs may serve as markers for tumors and hypoxia. A subset of CAs lack CA activity due to point mutations but retain esterase function.

Publications for Carbonic Anhydrase IX/CA9 (DY2188)(3)

Publications using DY2188

• K Grossmanno, M Barathova, P Belvonciko, V Lauko, L Csaderova, J Tomka, T Dulka, J Pastorek, J Madaric Hypoxia Marker Carbonic Anhydrase IX Is Present in Abdominal Aortic Aneurysm Tissue and Plasma International Journal of Molecular Sciences, 2022-01-14;23(2):. 2022-01-14 [PMID: 35055064] (Human)
• JAF Demandt, LJ Dubois, K van Kuijk, M Za?ovi?ová, H Jin, S Parkkila, SW van der La, L Jelenska, BME Mees, CPM Reutelings, KBJM Cleutjens, CJH van der Ka, CG Schalkwijk, MMJ van Greeve, EAL Biessen, G Pasterkamp, S Pastorekov, CDA Stehouwer, JC Sluimer The hypoxia-sensor carbonic anhydrase IX affects macrophage metabolism, but is not a suitable biomarker for human cardiovascular disease Scientific Reports, 2021-01-11;11(1):425. 2021-01-11 [PMID: 33432108] (Human)
• Dungwa JV, Hunt LP, Ramani P Overexpression of carbonic anhydrase and HIF-1alpha in Wilms tumours. BMC Cancer, 2011-09-12;11(0):390. 2011-09-12 [PMID: 21910893] (Human)

FAQs for Carbonic Anhydrase IX/CA9 (DY2188). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CA9