HuC Antibody - BSA Free

Western Blot: HuC Antibody - Azide and BSA Free [HuC] - Western blot analysis of various lysates, using HuC Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.8s.

Immunohistochemistry-Paraffin: HuC Antibody [NBP3-02943] - Immunohistochemistry of paraffin-embedded mouse brain using HuC antibody (NBP3-02943) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: HuC Antibody [NBP3-02943] - Paraffin-embedded rat brain using HuC antibody at dilution of 1:200 (40x lens).

Immunocytochemistry/ Immunofluorescence: HuC Antibody - Azide and BSA Free [HuC] - Immunofluorescence analysis of paraffin-embedded mouse brain using HuC Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HuC Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HuC Antibody - BSA Free (NBP3-02943) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 161-260 of human HuC (NP_001411.2). KTGQALLTHLYQSSAR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELAVL3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HuC Antibody - BSA Free

• DKFZp547J036
• ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C)
• ELAV-like protein 3
• Hu antigen C
• Hu-antigen C
• HuC
• HUCL
• HUCParaneoplastic cerebellar degeneration-associated antigen
• MGC20653
• PLE21Paraneoplastic limbic encephalitis antigen 21

Background

HuC is a member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: ELAVL3