ELAVL2 Antibody (CL15323) [Unconjugated] - BSA Free

Staining of mouse cerebral cortex shows strong cytoplasmic positivity in neurons.

Staining of human cerebellum shows strong cytoplasmic positivity in a subset of neurons in the granular layer.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of rat hippocampus shows strong cytoplasmic positivity in the dentate gyrus neurons.

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-43661 antibody. Corresponding ELAVL2 RNA-seq data are presented for the same tissues.

Staining of rat cerebral cortex shows strong cytoplasmic positivity in a subset of neurons.

Analysis in human cell line SCLC-21H.

Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferous ducts.

Staining in A-431 cell line with ELAVL2 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL15323
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ELAVL2 Antibody (CL15323) [Unconjugated] - BSA Free Summary

• Description: Novus Biologicals Mouse ELAVL2 Antibody (CL15323) [Unconjugated] - BSA Free (NBP3-43661) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of Q12926, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: ELAVL2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000-1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ELAVL2 Antibody (CL15323) [Unconjugated] - BSA Free

• ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
• ELAV-like neuronal protein 1
• ELAV-like protein 2
• HELN1
• HEL-N1
• Hu-antigen B
• HuB
• HUBELAV (embryonic lethal, abnormal vision, Drosophila)-like 2
• Nervous system-specific RNA-binding protein Hel-N1

Background

Hu-antigen B (HuB), ELAV-like protein 2, ELAV-like neuronal protein 1, Nervous system-specific RNA binding protein Hel-N1 is a transcription factor belonging to the ELAV family of RNP proteins. It binds RNA and seems to recognize a GAAA motif and is neuronal specific.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELAVL2