HuR/ELAVL1 Antibody (9Q6L3)

Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] - Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (NBP3-15820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] - Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (NBP3-15820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Immunocytochemistry/ Immunofluorescence: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Confocal imaging of NIH/3T3 cells using [KO Validated] HuR/ELAVL1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunoprecipitation: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug HuR/ELAVL1 antibody . Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody at a dilution of 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP, KO
• Clone: 9Q6L3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

HuR/ELAVL1 Antibody (9Q6L3) Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit HuR/ELAVL1 Antibody (9Q6L3) (NBP3-15820) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (Q15717). MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSS
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ELAVL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Immunoprecipitation 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • Knockout Validated
  • Western Blot 1:1000 - 1:6000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HuR/ELAVL1 Antibody (9Q6L3)

• ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• ELAV-like protein 1
• embryonic lethal, abnormal vision, drosophila, homolog-like 1
• Hu antigen R
• Hua
• Hu-antigen R
• HuR
• HURELAV1
• MelG

Background

HuR / ELAVL1 is encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELAVL1