HTRA2/Omi Antibody (196C429)

Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558] - Detection of HtrA2 using an HtrA2 monoclonal antibody. HL-60 cell lysate was probed with HtrA2 antibody at 3 ug/ml and two protein bands of approximate molecular weight of 50 and 38 kDa were detected. The 50 kDa and 38 kDa bands may represent precursor and mature forms of HtrA2.

Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558] - Analysis of HtrA2 using an HtrA2 monoclonal antibody. Probing with HtrA2 antibody shows staining in stomach tumor tissue (A) and very weak staining in normal stomach tissue (B).

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 196C429
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HTRA2/Omi Antibody (196C429) Summary

• Description: Novus Biologicals Mouse HTRA2/Omi Antibody (196C429) (NB100-56558) is a monoclonal antibody validated for use in IHC and WB. Anti-HTRA2/Omi Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 335-350 of human HtrA2.
• Specificity: This antibody does not cross-react with mouse HtrA2.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HTRA2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin reported in scientific literature (Lee et al (2003))
  • Western Blot 2-3 ug/ml
• Control:

  Jurkat Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for HTRA2/Omi Antibody (196C429)

• High temperature requirement protein A2
• HtrA serine peptidase 2
• HTRA2
• HtrA-like serine protease
• Omi
• OMIOmi stress-regulated endoprotease
• PARK13EC 3.4.21.108
• PRSS25
• PRSS25serine, 25
• Serine protease 25
• serine protease HTRA2, mitochondrial
• Serine proteinase OMI

Background

The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP. Like Smac/DIABLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HTRA2/Omi Antibody (NB100-56558)(3)

Publications using NB100-56558

• Laforge M, Bidere N, Carmona S et al. Apoptotic death concurrent with CD3 stimulation in primary human CD8+ T lymphocytes: a role for endogenous granzyme B. J Immunol. 2006-04-01 [PMID: 16547231] (WB)
  
  Details:
  WB: Cytosolic extracts from purified CD8+ T cells, Fig 3B.
• Wang X, Zhu C, Wang X et al. X-linked inhibitor of apoptosis (XIAP) protein protects against caspase activation and tissue loss after neonatal hypoxia-ischemia. Neurobiol Dis. 2004-06-01 [PMID: 15207275] (IF/IHC)
• Lee SH, Lee JW, Kim HS et al. Immunohistochemical analysis of Omi/HtrA2 expression in stomach cancer APMIS 2003-05-01 [PMID: 12887511] (IF/IHC)
  
  Details:
  This citation used the Biotin version of this antibody.

Bioinformatics

• Gene Symbol: HTRA2