Endo G Antibody

Images

 
Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human Endo G recombinant protein per lane. Antibodies: Endo G NBP1-76657, 1h incubation at RT in 5% ...read more
Immunohistochemistry: Endo G Antibody [NBP1-76657] - Analysis of human pancreas tissue with EndoG antibody at 2.5 ug/ml.
Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation in Mouse 3T3 (M) and Human HepG2 (H) cell lysates. Loading: 15 ug of lysates per lane. Antibodies: Endo G NBP1-76657 (2 ug/ml), 1h incubation at RT in ...read more
Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation in Human A431 Cell Lysate with the presence (A) or absence (B and C) of blocking peptide. Loading: 15 ug of lysates per lane. Antibodies: Endo G ...read more
Immunohistochemistry-Paraffin: Endo G Antibody [NBP1-76657] - Human pancreas with anti-Endo G at 15 ug/ml.
Immunofluorescence: Endo G Antibody [NBP1-76657] - Human pancreas tissue with EndoG antibody at 20 ug/mL. Green: EndoG Antibody Blue: DAPI staining

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Endo G Antibody Summary

Immunogen
Antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG. The immunogen is located within amino acids 40 - 90 of EndoG. Amino Acid Squence: GGPRGPGELAKYGLP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ENDOG
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Immunofluorescence
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Endo G Peptide (NBP1-76657PEP)
Publications
Read Publication using
NBP1-76657 in the following applications:

  • WB
    1 publication

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 25843897)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Endo G Antibody

  • EC 3.1.30.-
  • Endo G
  • endonuclease G
  • endonuclease G, mitochondrial
  • FLJ27463
  • mitochondrial endonuclease G

Background

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1457
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, KO
NB100-56503
Species: Hu, Mu, Rt, Dr, Rb
Applications: WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, Flow-CS, Flow-IC
NB100-56708
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
NBP1-28566
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, Func, IHC, IHC-Fr
NB500-213
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IP
NB100-56098
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P, IP
AF1458
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
NB100-56118
Species: Hu, Mu, Rt, Ca, Ma
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
AF860
Species: Hu, Mu
Applications: WB, Simple Western, IP
NB100-56104
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-56599
Species: Hu, Mu, Rt(-)
Applications: WB, Flow, ICC/IF, Flow-IC
NB100-56116
Species: Hu, Mu, Rt, Ma
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-77009
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-56074
Species: Hu, Mu, Rt, Ma
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-67460
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB600-1335
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, GS, ICC/IF, IHC, IHC-P, IP, ChIP, KD
NBP2-43728
Species: Hu, Mu, Rt
Applications: WB, ICC/IF

Publications for Endo G Antibody (NBP1-76657)(1)

Reviews for Endo G Antibody (NBP1-76657) (0)

There are no reviews for Endo G Antibody (NBP1-76657). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Endo G Antibody (NBP1-76657) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Endo G Products

Bioinformatics Tool for Endo G Antibody (NBP1-76657)

Discover related pathways, diseases and genes to Endo G Antibody (NBP1-76657). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Endo G Antibody (NBP1-76657)

Discover more about diseases related to Endo G Antibody (NBP1-76657).
 

Pathways for Endo G Antibody (NBP1-76657)

View related products by pathway.

PTMs for Endo G Antibody (NBP1-76657)

Learn more about PTMs related to Endo G Antibody (NBP1-76657).
 

Research Areas for Endo G Antibody (NBP1-76657)

Find related products by research area.

Blogs on Endo G

There are no specific blogs for Endo G, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Endo G Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ENDOG
Entrez
Uniprot