HTRA2/Omi Antibody (196C429) - Azide and BSA Free

Western Blot: HTRA2/Omi Antibody (196C429) - Azide and BSA Free [NBP2-80782] - Detection of HtrA2 using an HtrA2 monoclonal antibody. HL-60 cell lysate was probed with HtrA2 antibody at 3 ug/ml and two protein bands of approximate molecular weight of 50 and 38 kDa were detected. The 50 kDa and 38 kDa bands may represent precursor and mature forms of HtrA2. Image from the standard format of this antibody.

Immunohistochemistry: HTRA2/Omi Antibody (196C429) - Azide and BSA Free [NBP2-80782] - Analysis of HtrA2 using an HtrA2 monoclonal antibody. Probing with HtrA2 antibody shows staining in stomach tumor tissue (A) and very weak staining in normal stomach tissue (B). Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 196C429
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HTRA2/Omi Antibody (196C429) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HTRA2/Omi Antibody (196C429) - Azide and BSA Free (NB100-56558) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 335-350 of human HtrA2.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HTRA2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (Lee et al (2003))
  • Western Blot 2-3 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for HTRA2/Omi Antibody (196C429) - Azide and BSA Free

• High temperature requirement protein A2
• HtrA serine peptidase 2
• HTRA2
• HtrA-like serine protease
• Omi
• OMIOmi stress-regulated endoprotease
• PARK13EC 3.4.21.108
• PRSS25
• PRSS25serine, 25
• Serine protease 25
• serine protease HTRA2, mitochondrial
• Serine proteinase OMI

Background

The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP. Like Smac/DIABLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for HTRA2/Omi Antibody (NBP2-80782). (Showing 1 - 2 of 2 FAQ).

1. I would like to use this antibody but it has not been validated in my species of interest. Is there any way I can find out if it will work?
   • We offer risk-free testing of all of our primary antibodies. Please check out our Innovator's Reward Program and test this EGLN1-PHD2 antibody in any unvalidated species or application, without the financial risk of failure.
2. How do I choose secondary antibodies to label the same cells when I have two primary antibodies from the same host?
   • Use isotype-specific secondary antibodies if the primary antibodies are of different isotypes. You can also make direct conjugates of the primary antibodies by use of antibody labeling kits, dyes, or custom conjugations (please contact Technical Support for custom orders).

Bioinformatics

• Gene Symbol: HTRA2