HTRA1/PRSS11 Antibody (275615) [Unconjugated]

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HTRA1/PRSS11 Monoclonal Antibody (Catalog # MAB2916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HTRA1/PRSS11 at approximately 51 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

HtrA1 Protein Expression in hBC cell lines.Cells (as indicated) were extracted into nuclear (N) and cytoplasmic (C) fractions as described (Materials & Methods), and proteins were analyzed by Immunoblot analyses using the polyclonal antibody against human HtrA1. beta -actin was used as a loading control, and DEK was used to assess the nuclear/cytoplasmic fractionation (DEK is exclusively nuclear). The larger Mr bands seen in the MCF12A and MCF10A/Flp cell lines are consistent with dimers, trimers, etc., although this was not confirmed. MCF10A/Flp is the parental Flp-in cell line which was used to produce the various MCF10A/siRNA or Htra1 cell lines. Results are from a representative experiment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22761798), licensed under a CC-BY license. Not internally tested by R&D Systems.

Characterization of MCF10A cell lines carrying HtrA1 siRNA and overexpression vectors.A random antisense oligonucleotide library was used to identify optimally accessible sites in HtrA1 mRNA. SiRNAs (short hairpin RNAs) were designed to target these sites, and stably transfected cell lines were developed from MCF10A cells (4 independent cell lines, designated MCF10A/siRNA1-4). A control cell line expressing an irrelevant siRNA (designated MCF10A/HPVsh) was also developed. In parallel, we also developed a cell line over-expressing HtrA1 (designated MCF10A/HtrA1). (A). Target sites empirically identified in HtrA1 mRNA. Identified domains within HtrA1 include: SS, signal sequence; IGFBP, IGF binding site; KI, Kazal Type I protease inhibitor domain; Trypsin-like protease domain; PDZ, PDZ binding domain. (B) Immunoblot analysis for HtrA1 protein. Cytoplasmic and nuclear protein fractions were prepared from the developed cell lines (as indicated), and were probed with a polyclonal antibody preparation directed against a region in the trypsin-like protease domain. As is evident, the reductions in HtrA1 protein levels were >90% compared with the various MCF10A and MCF10A/HPVsh cells. Right panel shows HtrA1 in concentrated culture medium. (C) Cells were plated and growth was measured over a 6 day period. Both of the MCF10A cell lines tested (MCF10A/siRNA1 and siRNA4) grew significantly faster than the control cells (p<0.01 at days 4 and 6). Over-expression of HtrA1 in the MCF10A/HtrA1 cells had no effect on cell growth rate. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22761798), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IP
• Clone: 275615
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HTRA1/PRSS11 Antibody (275615) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human HTRA1/PRSS11
  Gln23-Pro480
  Accession # Q92743
• Specificity: Detects human HTRA1/PRSS11 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HTRA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HTRA1/PRSS11 Antibody (275615) [Unconjugated]

• ARMD7
• EC 3.4.21
• EC 3.4.21.108
• High-temperature requirement A serine peptidase 1
• HtrA serine peptidase 1
• HTRA1
• IGFBP5-protease
• L56
• ORF480
• PRSS11
• Serine protease 11
• serine, 11 (IGF binding)

Background

HTRA1 is a serine protease encoded by the PRSS11 gene and is expressed in many tissues. HTRA1 regulates the availability of IGFs by cleaving IGFBPs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HTRA1/PRSS11 Antibody (MAB2916)(16)

Publications using MAB2916

• Navneet, S;Ishii, M;Rohrer, B; Altered Elastin Turnover, Immune Response, and Age-Related Retinal Thinning in a Transgenic Mouse Model With RPE-Specific HTRA1 Overexpression Investigative ophthalmology & visual science 2024-07-01 [PMID: 39028977] (Immunohistochemistry, Transgenic Mouse)
• Beaufort, N;Ingendahl, L;Merdanovic, M;Schmidt, A;Podlesainski, D;Richter, T;Neumann, T;Kuszner, M;Vetter, IR;Stege, P;Burston, SG;Filipovic, A;Ruiz-Blanco, YB;Bravo-Rodriguez, K;Mieres-Perez, J;Beuck, C;Uebel, S;Zobawa, M;Schillinger, J;Malik, R;Todorov-Völgyi, K;Rey, J;Roberti, A;Hagemeier, B;Wefers, B;Müller, SA;Wurst, W;Sanchez-Garcia, E;Zimmermann, A;Hu, XY;Clausen, T;Huber, R;Lichtenthaler, SF;Schmuck, C;Giese, M;Kaiser, M;Ehrmann, M;Dichgans, M; Rational correction of pathogenic conformational defects in HTRA1 Nature communications 2024-07-16 [PMID: 39013852] (Western Blot, Mouse)
• Schrader, JM;Xu, F;Agostinucci, KJ;DaSilva, NA;Van Nostrand, WE; Longitudinal markers of cerebral amyloid angiopathy and related inflammation in rTg-DI rats Scientific reports 2024-04-10 [PMID: 38600214] (Immunohistochemistry, Rat)
• Kouri, AM;Caza, TN;Beck, LH;Misurac, JM;Evans, MD;Phillips, CL;Eadon, MT;Larsen, CP;Andreoli, SP;Bu, L;Rheault, MN;Khalid, M; Clinical and Histopathologic Characteristics of Pediatric Patients With Primary Membranous Nephropathy Kidney international reports 2023-11-01 [PMID: 38025223] (IHC, Human)
• D Inoue, GL Chew, B Liu, BC Michel, J Pangallo, AR D'Avino, T Hitchman, K North, SC Lee, L Bitner, A Block, AR Moore, A Yoshimi, L Escobar-Ho, H Cho, A Penson, SX Lu, J Taylor, Y Chen, C Kadoch, O Abdel-Waha, RK Bradley Spliceosomal disruption of the non-canonical BAF complex in cancer Nature, 2019-10-09;0(0):. 2019-10-09 [PMID: 31597964] (Western Blot, Mouse)
• DC Hondius, KN Eigenhuis, THJ Morrema, RC van der Sc, P van Nierop, M Bugiani, KW Li, JJM Hoozemans, AB Smit, AJM Rozemuller Proteomics analysis identifies new markers associated with capillary cerebral amyloid angiopathy in Alzheimer&#039;s disease Acta Neuropathol Commun, 2018-06-04;6(1):46. 2018-06-04 [PMID: 29860944] (IHC, Human)
• S Yamawaki, M Naitoh, H Kubota, R Aya, Y Katayama, T Ishiko, T Tamura, K Yoshikawa, T Enoshiri, M Ikeda, S Suzuki HtrA1 Is Specifically Up-Regulated in Active Keloid Lesions and Stimulates Keloid Development Int J Mol Sci, 2018-04-24;19(5):. 2018-04-24 [PMID: 29695130] (Western Blot, Human)
• O Globus, T Evron, M Caspi, R Siman-Tov, R Rosin-Arbe High-Temperature Requirement A1 (Htra1) - A Novel Regulator of Canonical Wnt Signaling Sci Rep, 2017-12-21;7(1):17995. 2017-12-21 [PMID: 29269789] (Western Blot, Human)
• E Melo, P Oertle, C Trepp, H Meisterman, T Burgoyne, L Sborgi, AC Cabrera, CY Chen, JC Hoflack, T Kam-Thong, R Schmucki, L Badi, N Flint, ZE Ghiani, F Delobel, C Stucki, G Gromo, A Einhaus, B Hornsperge, S Golling, J Siebourg-P, F Gerber, B Bohrmann, C Futter, T Dunkley, S Hiller, O Schilling, V Enzmann, S Fauser, M Plodinec, R Iacone HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model EBioMedicine, 2017-12-13;0(0):. 2017-12-13 [PMID: 29269042] (Immunoprecipitation, Mouse)
• Chi Pui Pang Protective effects of an HTRA1 insertion-deletion variant against age-related macular degeneration in the Chinese populations Lab. Invest., 2016-11-14;0(0):. 2016-11-14 [PMID: 27841854] (ICC, Western Blot, Human)
• Tsz Kin Ng HTRA1 promoter variant differentiates polypoidal choroidal vasculopathy from exudative age-related macular degeneration Sci Rep, 2016-06-24;6(0):28639. 2016-06-24 [PMID: 27338780] (Western Blot, Human)
• Jo , Hakryul, Patterson , Victoria, Stoessel , Sean, Kuan , Chia-Yi, Hoh , Josephin Protoporphyrins enhance oligomerization and enzymatic activity of HtrA1 serine protease. PLoS ONE, 2014-12-15;9(12):e115362. 2014-12-15 [PMID: 25506911] (Western Blot, Human)
• Kumar , Sandeep, Berriochoa , Zachary, Ambati , Balamura, Fu , Yingbin Angiographic features of transgenic mice with increased expression of human serine protease HTRA1 in retinal pigment epithelium. Invest Ophthalmol Vis Sci, 2014-05-22;55(6):3842-50. 2014-05-22 [PMID: 24854852] (IHC, Western Blot, Mouse)
• Jacobo , Sarah Me, Deangelis , Margaret, Kim , Ivana K, Kazlauskas , Andrius Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair its ability to antagonize insulin-like growth factor 1. Mol Cell Biol, 2013-03-11;33(10):1976-90. 2013-03-11 [PMID: 23478260] (Immunoprecipitation, Human)
• Wang N, Eckert KA, Zomorrodi AR Down-Regulation of HtrA1 Activates the Epithelial-Mesenchymal Transition and ATM DNA Damage Response Pathways. PLoS ONE, 2012-06-26;7(6):e39446. 2012-06-26 [PMID: 22761798] (Western Blot, Human)
• Mauney J, Olsen BR, Volloch V Matrix remodeling as stem cell recruitment event: a novel in vitro model for homing of human bone marrow stromal cells to the site of injury shows crucial role of extracellular collagen matrix. Matrix Biol., 2010-09-07;29(8):657-63. 2010-09-07 [PMID: 20828613] (Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: HTRA1
• Uniprot:
  • Q92743()