HspA6 Antibody - BSA Free

Western Blot: HspA6 Antibody [NBP1-32761] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000

Immunocytochemistry/Immunofluorescence: HspA6 Antibody [NBP1-32761] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-32761] - Paraffin-embedded SG xenograft, using antibody at 1:500 dilution.

Western Blot: HspA6 Antibody [NBP1-32761] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HspA6 antibody [C1C3] (NBP1-32761) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HspA6 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HspA6. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPA6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HspA6 Antibody - BSA Free

• heat shock 70 kDa protein 6
• Heat shock 70 kDa protein B'
• heat shock 70kD protein 6 (HSP70B')
• heat shock 70kDa protein 6 (HSP70B')
• HSP70B'

Background

HSP70B is a unique member of the human Hsp70 chaperone involved in cellular processes such as protein trafficking, folding, and prevention of aggregation. This protein is strictly stress-inducible, having little or no basal expression levels in most cells. HSP70B and Hsp72 are closely related and play cooperative roles in cell survival of proteotoxic stress. Recombinant human HSP70B, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for HspA6 Antibody (NBP1-32761)(1)

Publication using NBP1-32761

• Ji HW, Kim H, Kim HW et al. Genome-Wide Comparison of the Target Genes of the Reactive Oxygen Species and Non-Reactive Oxygen Species Constituents of Cold Atmospheric Plasma in Cancer Cells Cancers 2020-09-16 [PMID: 32947888] (WB)

Bioinformatics

• Gene Symbol: HSPA6