HDJ2 Antibody

Images

 
Genetic Strategies: Western Blot: HDJ2 Antibody [NBP1-88019] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: HDJ2 Antibody [NBP1-88019] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubules.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

HDJ2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGE
Specificity
Specificity of human, mouse, rat HDJ2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNAJA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04 - 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control
HDJ2 Knockout 293T Cell Lysate
Control Peptide
HDJ2 Protein (NBP1-88019PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HDJ2 Antibody

  • dj-2
  • DjA1
  • DnaJ (Hsp40) homolog, subfamily A, member 1
  • dnaJ homolog subfamily A member 1
  • DnaJ protein homolog 2
  • DNAJ2
  • HDJ2
  • hDJ-2
  • Heat shock 40 kDa protein 4
  • Heat shock protein J2
  • heat shock protein, DNAJ-like 2
  • HSDJ
  • HSJ2
  • HSJ-2
  • HSPF4hdj-2
  • Human DnaJ protein 2
  • NEDD7
  • neural precursor cell expressed, developmentally down-regulated 7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HDJ2 Antibody (NBP1-88019) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for HDJ2 Antibody (NBP1-88019)

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Bioinformatics

Gene Symbol DNAJA1