HDJ2 Antibody

Images

 
Genetic Strategies: Western Blot: HDJ2 Antibody [NBP1-88019] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: HDJ2 Antibody [NBP1-88019] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubules.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

HDJ2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNAJA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Knockdown Validated
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
HDJ2 Protein (NBP1-88019PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HDJ2 Antibody

  • dj-2
  • DjA1
  • DnaJ (Hsp40) homolog, subfamily A, member 1
  • dnaJ homolog subfamily A member 1
  • DnaJ protein homolog 2
  • DNAJ2
  • HDJ2
  • hDJ-2
  • Heat shock 40 kDa protein 4
  • Heat shock protein J2
  • heat shock protein, DNAJ-like 2
  • HSDJ
  • HSJ2
  • HSJ-2
  • HSPF4hdj-2
  • Human DnaJ protein 2
  • NEDD7
  • neural precursor cell expressed, developmentally down-regulated 7

Background

HDJ 2 is a novel human homolog of E. coli's DNA J protein from heat shock protein 40 family. Hsp 40 is involved in chaperonic protein folding. HDJ-2 is cytoplasmic and nuclear membrane associated protein; upon heat shock it migrates and becomes associated with the golgi complex, nucleous and nuclear membrane. HDJ 2 has a prenylated C-terminus and is not inducible upon heat shock.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HDJ2 Antibody (NBP1-88019) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for HDJ2 Antibody (NBP1-88019)

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Blogs on HDJ2

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Bioinformatics

Gene Symbol DNAJA1