AlphaB Crystallin/CRYAB Antibody

Images

 
Biological Strategies: Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin ...read more
Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Gp, Pm, Rb, ShSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.08 mg/ml
Validated by:
   

Biological Strategies

     

AlphaB Crystallin/CRYAB Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human Crystallin AB protein (within residues 70-140). [Swiss-Prot P02511]
Localization
Cytoplasmic
Predicted Species
Rat (100%), Porcine (100%), Rabbit (100%), Guinea Pig (100%), Chicken (100%), Sheep (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRYAB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:150
Application Notes
This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. **CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Control
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
HSP27 Overexpression Lysate
Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
AlphaB Crystallin/CRYAB Peptide (NB100-2519PEP)
Publications
Read Publications using
NB100-2519 in the following applications:

Reactivity Notes

Predicted to react with rat, sheep, rabbit, monkey, guinea pig, chicken, and pig based on 100% sequence homology. Immunogen sequence has 81% homology with Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.08 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AlphaB Crystallin/CRYAB Antibody

  • alpha B crystallin
  • alpha(B)-crystallin
  • AlphaB Crystallin
  • alpha-crystallin B chain
  • CRYA2
  • CRYA2alpha crystallin B chain
  • CRYAB
  • crystallin, alpha B
  • CTPP2
  • Heat shock protein beta-5
  • heat-shock 20 kD like-protein
  • HSPB5
  • Renal carcinoma antigen NY-REN-27
  • Rosenthal fiber component

Background

Crystallin AB (CRYAB or alpha-basic-crystallin) belongs to HSP20 family and was originally discovered as chaperone abundantly expressed in eye lens where it plays a key role in maintaining transparency and refractive index of lens. Subsequently, CRYAB was found to acts as a molecular chaperone induced by a variety of stress stimuli, conferring cytoprotection by suppressing aggregation of denatured protein. In its anti-apoptotic role, CRYAB protects cells against stress, TNF-alpha, apoptotic effectors etc. and can inhibit caspase-3/PARP alongwith preventing Bax/Bcl-2 translocation. CRYAB is a heteropolymer of three CRYAA and one CRYAB subunits, and can aggregate with homologous proteins (e.g. HSPB1) to form large complexes and interact with HSPBAP1 as well as TTN/titin. Localized in cytoplasm, during heat shock stimulus, CRYAB translocates to sub-nuclear structures known as SC35/nuclear splicing speckles. CRYAB is expressed in several other tissues such as muscle, brain, spleen, lung, kidney, skin etc and has been shown to implicate in certain diseases including cardiac myopathies, neurodegenerative diseases and malignancies including brain, breast, prostate and renal cancers. Defectsive CRYAB causes myopathy myofibrillar type 2 (MFM2), cataract posterior polar type 2 (CTPP2) and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AlphaB Crystallin/CRYAB Antibody (NB100-2519)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Filter By Species
Mouse
(1)
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Product General Protocols

View specific protocols for AlphaB Crystallin/CRYAB Antibody (NB100-2519): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AlphaB Crystallin/CRYAB Antibody (NB100-2519) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2519AF350
Alexa Fluor 405 NB100-2519AF405
Alexa Fluor 488 NB100-2519AF488
Alexa Fluor 532 NB100-2519AF532
Alexa Fluor 594 NB100-2519AF594
Alexa Fluor 647 NB100-2519AF647
Alexa Fluor 700 NB100-2519AF700
Alexa Fluor 750 NB100-2519AF750
Biotin NB100-2519B
DyLight 350 NB100-2519UV
DyLight 405 NB100-2519V
DyLight 488 NB100-2519G
DyLight 550 NB100-2519R
DyLight 594 NB100-2519DL594
DyLight 650 NB100-2519C
DyLight 680 NB100-2519FR
DyLight 755 NB100-2519IR
FITC NB100-2519F
HRP NB100-2519H
Janelia Fluor 549 NB100-2519JF549
Janelia Fluor 646 NB100-2519JF646

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Bioinformatics

Gene Symbol CRYAB