AlphaB Crystallin/CRYAB Antibody - BSA Free

Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.

• Reactivity: Hu, Mu, Rt, Po, Ch, Gp, Pm, Rb, Sh
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.08 mg/ml

AlphaB Crystallin/CRYAB Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AlphaB Crystallin/CRYAB Antibody - BSA Free (NB100-2519) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-AlphaB Crystallin/CRYAB Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal region of the human Crystallin AB protein (within residues 70-140). [Swiss-Prot P02511]
• Localization: Cytoplasmic
• Predicted Species: Rat (100%), Porcine (100%), Rabbit (100%), Guinea Pig (100%), Chicken (100%), Sheep (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRYAB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:150. Use reported in scientific literature
  • Western Blot 0.5-1.0 ug/ml
• Application Notes: In Western blot analysis, a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. **CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes.
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  HSP27 Overexpression Lysate
  Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  AlphaB Crystallin/CRYAB Peptide (NB100-2519PEP)

Reactivity Notes

Predicted to react with rat, sheep, rabbit, monkey, guinea pig, chicken, and pig based on 100% sequence homology. Immunogen sequence has 81% homology with Zebrafish.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1.08 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AlphaB Crystallin/CRYAB Antibody - BSA Free

• alpha B crystallin
• alpha(B)-crystallin
• AlphaB Crystallin
• alpha-crystallin B chain
• CRYA2
• CRYA2alpha crystallin B chain
• CRYAB
• crystallin, alpha B
• CTPP2
• Heat shock protein beta-5
• heat-shock 20 kD like-protein
• HSPB5
• Renal carcinoma antigen NY-REN-27
• Rosenthal fiber component

Background

Crystallin AB (CRYAB or alpha-basic-crystallin) belongs to HSP20 family and was originally discovered as chaperone abundantly expressed in eye lens where it plays a key role in maintaining transparency and refractive index of lens. Subsequently, CRYAB was found to acts as a molecular chaperone induced by a variety of stress stimuli, conferring cytoprotection by suppressing aggregation of denatured protein. In its anti-apoptotic role, CRYAB protects cells against stress, TNF-alpha, apoptotic effectors etc. and can inhibit caspase-3/PARP alongwith preventing Bax/Bcl-2 translocation. CRYAB is a heteropolymer of three CRYAA and one CRYAB subunits, and can aggregate with homologous proteins (e.g. HSPB1) to form large complexes and interact with HSPBAP1 as well as TTN/titin. Localized in cytoplasm, during heat shock stimulus, CRYAB translocates to sub-nuclear structures known as SC35/nuclear splicing speckles. CRYAB is expressed in several other tissues such as muscle, brain, spleen, lung, kidney, skin etc and has been shown to implicate in certain diseases including cardiac myopathies, neurodegenerative diseases and malignancies including brain, breast, prostate and renal cancers. Defectsive CRYAB causes myopathy myofibrillar type 2 (MFM2), cataract posterior polar type 2 (CTPP2) and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AlphaB Crystallin/CRYAB Antibody (NB100-2519)(4)

Publications using NB100-2519

• Chen AT, Xiao Y, Tang X et al. Cross-platform analysis reveals cellular and molecular landscape of glioblastoma invasion Neuro-oncology 2022-07-28 [PMID: 35901838]
• Shaw A, TOth B, Arianti R Et al. BMP7 increases UCP1-dependent and independent thermogenesis with a unique gene expression program in human neck area derived adipocytes Pharmaceuticals 2021-09-28 [PMID: 34832860]
• Pignataro L, Varodayan FP, Tannenholz LE et al. Brief alcohol exposure alters transcription in astrocytes via the heat shock pathway. Brain Behav. 2013-03-01 [PMID: 23533150] (ICC/IF, Mouse)
• Liu L, Qi X, Chen Z et al. Targeting the IRE1alpha/XBP1 and ATF6 Arms of the Unfolded Protein Response Enhances VEGF Blockade to Prevent Retinal and Choroidal Neovascularization. Am J Pathol 2013-02-07 [PMID: 23395094]

Bioinformatics

• Gene Symbol: CRYAB