HSP90 beta Antibody (7G7) - BSA Free

Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot: HSP90 beta Antibody (7G7) [NBP3-26216] - Positive Western Blot detected in: HepG2 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate. All lanes: HSP90 beta Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 84 kDa Observed band size: 80-90 kDa

Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (7G7) [NBP3-26216] - Staining of HepG2 cell with HSP90 beta Antibody (7G7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 495-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: HSP90 beta Antibody (7G7) [NBP3-26216] - Overlay Peak curve showing Hela cells stained with HSP90 beta Antibody (7G7) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 7G7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HSP90 beta Antibody (7G7) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human HSP90 beta [UniProt P08238]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HSP90AB1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HSP90 beta Antibody (7G7) - BSA Free

• D6S182
• Heat shock 84 kDa
• heat shock 90kD protein 1, beta
• heat shock 90kDa protein 1, beta
• heat shock protein 90kDa alpha (cytosolic), class B member 1
• heat shock protein beta
• heat shock protein HSP 90-beta
• HSP 84
• HSP84
• HSP90 beta
• HSP90AB1
• HSP90B
• HSP90-BETA
• HSP90BHSP 90
• HSPC2
• HSPC2D6S182
• HSPCB
• HSPCBFLJ26984

Background

The 90-kDa heat shock protein (Hsp90) is a highly conserved and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine, serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform, (Hsp90-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSP90AB1