HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free

Western Blot: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp70.

Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded pancreas tissue using anti-Hsp70 mouse monoclonal antibody.

Flow Cytometry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Analysis of permeabilized Jurkat cells, using anti-HSPA1A antibody( Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

Western Blot: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp70 monoclonal antibody.

Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Hsp70 mouse monoclonal antibody.

Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded endometrium tissue using anti-Hsp70 mouse monoclonal antibody.

Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded Kidney tissue using anti-Hsp70 mouse monoclonal antibody.

Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded liver tissue using anti-Hsp70 mouse monoclonal antibody.

Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded prostate tissue using anti-Hsp70 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI3G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free (NBP2-02190) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA1A