HSP70/HSPA1A Antibody (C92F3A-5)

Images

 
Western Blot: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92. Load: 15 ug ...read more
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours ...read more
Flow Cytometry: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92 (SMC-100). Tissue: Heat Shocked CD3+ CD8+ T ...read more
Immunohistochemistry: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92. Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse ...read more
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ce, Ch, Dr, Fi, Gp, Ha, Pm, Rb, ShSpecies Glossary
Applications WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-P, IM, MiAr, Binding Activity
Clone
C92F3A-5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HSP70/HSPA1A Antibody (C92F3A-5) Summary

Immunogen
Human HSP70
Localization
Cytoplasm
Specificity
Detects approx 70kDa. Does not cross-react with HSC70 (HSP73).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Electron Microscopy
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:10000
  • Immunohistochemistry-Paraffin 1:10000
  • Immunomicroscopy
  • Microarray
  • Binding Activity
Application Notes
1 ug/ml of HSP70 Antibody was sufficient for detection of HSP70 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Reviewed Applications
Read 1 Review rated 5
using
NB110-61582 in the following applications:

Publications
Read Publications using
NB110-61582 in the following applications:

  • WB
    2 publications

Reactivity Notes

Porcine reactivity reported in (PMID: 23624345).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HSP70/HSPA1A Antibody (C92F3A-5)

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsp70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-61582
Species: Hu, Mu, Rt, Po, Bv, Ca, Ce, Ch, Dr, Fi, Gp, Ha, Pm, Rb, Sh
Applications: WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-P, IM, MiAr, Binding Activity

Publications for HSP70/HSPA1A Antibody (NB110-61582)(5)

Review for HSP70/HSPA1A Antibody (NB110-61582) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-61582:
Filter by Applications
WB
(1)
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 05/14/2014
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Blocking

Blocking Detailsskim milk, 1hr, rt

Primary Anitbody

Dilution Ratio1:200, Over night, RT

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP70/HSPA1A Antibody (NB110-61582) (0)

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Secondary Antibodies

 

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Recent Reviews

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Anonymous
05/14/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HSPA1A
Entrez
Uniprot