HSP70/HSPA1A Antibody (5A5)

Images

 
Western Blot: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - analysis of Rat skeletal muscle tissue lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5A5. Load: 15 ug protein. ...read more
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
5A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HSP70/HSPA1A Antibody (5A5) Summary

Immunogen
Human recombinant HSP70 overexpressed in E.coli detects several members of the heat shock protein 70kDa gene family including Hsp70, Hsc70, Grp78 and following heat shock, Hsp72. IF staining of Hsp70 in heat shocked HeLa cells results in cytoplasmic staining.
Localization
Cytoplasm
Specificity
Detects approx 70kDa. May detect HSP70, HSC70, Grp78 and HSP72.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1ug
Application Notes
1 ug/ml of HSP70 Antibody was sufficient for detection of HSP70 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Publications
Read Publications using NBP1-21690.

Reactivity Notes

Based on homology, It's predicted to detect Mouse, Rat, Amphibians, Chicken, Fish, Yeast (Saccharomyces cerevisiae) and Drosophila.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HSP70/HSPA1A Antibody (5A5)

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

Detects several members of the heat shock protein 70kDa gene family including Hsp70, Hsc70, Grp78 and following heat shock, Hsp72. IF staining of Hsp70 in heat shocked HeLa cells results in cytoplasmic staining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP70/HSPA1A Antibody (NBP1-21690)(6)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP70/HSPA1A Antibody (NBP1-21690). (Showing 1 - 1 of 1 FAQs).

  1. What is the molecular weight of the antibody sold by your company for product 5A5 monoclonal antibody against heat shock protein 70?
    • This product detects multiple heat shock proteins, all of which are detected at around 70 kDa. If you're asking about the molecular weight of the antibody itself, this is a mouse monoclonal IgG1 antibody which has a weight of around 150 kDa.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HSPA1A
Entrez
Uniprot