HSP70/HSPA1A Antibody (5A5) - BSA Free

Western Blot: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - analysis of Rat skeletal muscle tissue lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5A5 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.

• Reactivity: Hu, Mu, Rt, Am, Ch, Dr, Fi, Ye
• Applications: WB, Flow, ICC/IF, IHC, IP, MA
• Clone: 5A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HSP70/HSPA1A Antibody (5A5) - BSA Free Summary

• Description: Novus Biologicals Mouse HSP70/HSPA1A Antibody (5A5) - BSA Free (NBP1-21690) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-HSP70/HSPA1A Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant HSP70 overexpressed in E.coli
• Localization: Cytoplasm
• Specificity: Detects approx 70kDa. May detect HSP70, HSC70, Grp78 and HSP72.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1ug
  • Microarray
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of HSP70 Antibody was sufficient for detection of HSP70 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. Use in Flow reported in scientific literature (PMID:33513976)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HSP70/HSPA1A Antibody (5A5) - BSA Free

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Detects several members of the heat shock protein 70kDa gene family including Hsp70, Hsc70, Grp78 and following heat shock, Hsp72. IF staining of Hsp70 in heat shocked HeLa cells results in cytoplasmic staining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP70/HSPA1A Antibody (NBP1-21690)(8)

Publications using NBP1-21690

• Ferguson Bennit HR, Gonda A, Kabagwira J, et al. Natural Killer Cell Phenotype and Functionality Affected by Exposure to Extracellular Survivin and Lymphoma-Derived Exosomes International journal of molecular sciences 2021-01-27 [PMID: 33513976] (FLOW, Human)
• Lucas TA, Johns G, Jiang W, Yang L. A population model of chaparral vegetation response to frequent wildfires. Bulletin of mathematical biology 2014-08-13 [PMID: 24091781]
• Balashova N, Chang FJ, Lamothe M, Sun Q, Beuve A. Characterization of a novel type of endogenous activator of soluble guanylyl cyclase. J Biol Chem 280:2186-96. 2005-01-01 [PMID: 15509556] (IP)
• Fink AL. Chaperone-mediated protein folding. Physiol. Rev. 79: 425-449. 1999-01-01 [PMID: 10221986]
• Boorstein W R, Ziegelhoffer T, Craig E A. Molecular evolution of the HSP70 multigene family. J. Mol. Evol.38 (1): 1-17. 1993-01-01 [PMID: 8151709]
• Rothman J. Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells. Cell 59: 591 -601. 1989-01-01 [PMID: 2573430]
• DeLuca-Flaherty et al. Uncoating protein (hsc70) binds a conformationally labile domain of clathrin light chain LCa to stimulate ATP hydrolysis. Cell 62: 875-887. 1990-01-01 [PMID: 1975516]
• Bork P, Sander C & Valencia A. An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins. Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 1992-01-01 [PMID: 1323828]

FAQs for HSP70/HSPA1A Antibody (NBP1-21690). (Showing 1 - 1 of 1 FAQs).

1. What is the molecular weight of the antibody sold by your company for product 5A5 monoclonal antibody against heat shock protein 70?
   • This product detects multiple heat shock proteins, all of which are detected at around 70 kDa. If you're asking about the molecular weight of the antibody itself, this is a mouse monoclonal IgG1 antibody which has a weight of around 150 kDa.

Bioinformatics

• Gene Symbol: HSPA1A
• Entrez:
  • 3304(Human)
• Uniprot:
  • P08107()