HSP70/HSPA1A Antibody (1H11) - BSA Free

Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis on non-permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate showing cell membrane staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.

Flow (Cell Surface): HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - A cell surface stain was performed on HeLa cells with Hsp70 / Hsc70 Antibody (1H11) NBP2-59339PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Hsp70 / Hsc70 Antibody (1H11) [NBP2-59339] - Clone 1H11: FITC conjugate. Tissue: Jurkat E6.1. Species: Human. Unfixed/non-permeablized cells were incubated with (A) Anti-HSP70 control antibody (not cell-surface-specific), or (B) Anti-HSP70 antibody, clone 1H11 for 40 min at 4 degrees C. Fixed/permeabilized cells were incubated with (C) Anti-HSP70 control antibody, or (D) Anti-HSP70 antibody, clone 1H11 or 40 min at 4 degrees C. Analysis shows that non-permeabilized cells show cell surface binding with 1H11 clone (NBP2-59339) but not with the control antibody, and the permeabilized cells show strong intracellular binding with the control antibody but much less with 1H11 clone (NBP2-59339). Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, Flow, ICC/IF
• Clone: 1H11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

HSP70/HSPA1A Antibody (1H11) - BSA Free Summary

• Immunogen: Human native HSP70 protein
• Localization: Cell membrane
• Specificity: Binds the altered conformation of HSP70 embedded in the cell membrane and to intracellular HSP70 to a lesser extent.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for HSP70/HSPA1A Antibody (1H11) - BSA Free

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA1A