HRD1 Antibody

Images

 
Western Blot: HRD1 Antibody [NB100-2526] - Detection of HRD1 in an endogenous 293T cell lysate.
Immunocytochemistry/ Immunofluorescence: HRD1 Antibody [NB100-2526] - HRD1 antibody was tested in Hek293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: HRD1 Antibody [NB100-2526] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human tonsil using rabbit polyclonal HRD1 antibody at 2ug/ml concentration (1:500 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HRD1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of human HRD1 (within residues 300-400). [UniProt Q96PK3]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYVN1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Knockout Validated
Application Notes
This HRD1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. Heat samples at 50 degrees C for 30 minutes to detect HRD1 by Western blot. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24453213) KO Validation was reported in scientific literature (PMID: 26551274).
Positive Control
HRD1 Lysate (NBL1-16659)
Publications
Read Publications using
NB100-2526 in the following applications:

  • 1 publication
  • WB
    8 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for HRD1 Antibody

  • E3 ubiquitin-protein ligase synoviolin
  • EC 6.3.2
  • HRD1EC 6.3.2.-
  • KIAA1810Synovial apoptosis inhibitor 1
  • MGC40372
  • synovial apoptosis inhibitor 1, synoviolin

Background

HRD1 (synovial apoptosis inhibitor 1 or SYVN1) is a ubiquitin ligase whose expression is induced by UPR following ER stress and it provides protection to cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in ER. HRD1 acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from ER-associated UBC7 E2 ligase and transfers it to target proteins for promoting their degradation. It is a component of ER's quality control system called ERAD (ER-associated degradation) which is involved in ubiquitin-dependent degradation of misfolded ER proteins. HRD1 also facilitates the degradation of normal/naturally short-lived proteins such as SGK, and has been shown to protect neuronal cells from apoptosis triggered by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by prooting their degradation. Moreover, HRD1 sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HRD1 Antibody (NB100-2526)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(8)
All Applications
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Human
(3)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB100-2526 Applications Species
Sun S, Shi G, Sha H et al. IRE1alpha is an endogenous substrate of endoplasmic-reticulum-associated degradation. Nat Cell Biol 2015 Dec [PMID: 26551274] (WB) WB
Sun S, Shi G, Sha H et al. IRE1 [alpha] is an endogenous substrate of endoplasmic-reticulum-associated degradation. Nature cell Biology. 2015 Nov 09 (WB, Mouse) WB Mouse
Xie H, Tang CA, Song JH, Mancuso A. IRE1a RNase-dependent lipid homeostasis promotes survival in Myc-transformed cancers. J. Clin. Invest. 2018 Feb 26 [PMID: 29381485] (WB, Human) WB Human
Hwang J, Walczak CP, Shaler TA et al. Characterization of protein complexes of the endoplasmic reticulum associated degradation E3 ubiquitin ligase Hrd1. J. Biol. Chem. Apr 14 2017 [PMID: 28411238] (WB, Human) WB Human
Koenig Pa, Nicholls Pk, Schmidt Fi et al. The E2 Ubiquitin Conjugating Enzyme UBE2J1 is Required for Spermiogenesis in Mice. J. Biol. Chem. 2014 Oct 15 [PMID: 25320092] (WB, Mouse) WB Mouse
Eshraghi A, Dixon SD, Tamilselvam B et al. Cytolethal Distending Toxins Require Components of the ER-Associated Degradation Pathway for Host Cell Entry PLoS Pathog. 2014 Jul 01 [PMID: 25078082]
Sun S, Shi G, Han X et al. Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival. Proc. Natl. Acad. Sci. U.S.A. 2014 Feb 5 [PMID: 24453213] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Francisco AB, Singh R, Sha H et al. Haploid insufficiency of suppressor enhancer Lin12 1 like (SEL1L) predisposes mice to high fat diet-induced hyperglycemia. J Biol Chem;286(25):22275-82. 2011 Jun 24. [PMID: 21536682]
Dougan SK, Hu CC, Paquet ME et al. Derlin-2-deficient mice reveal an essential role for protein dislocation in chondrocytes Mol Cell Biol 2011 Mar [PMID: 21220515] (WB, Mouse) WB Mouse
Soundararajan R, Wang J, Melters D, Pearce D. Glucocorticoid-induced Leucine zipper 1 stimulates the epithelial sodium channel by regulating serum- and glucocorticoid-induced kinase 1 stability and subcellular localization. J Biol Chem 2010 Dec 17 [PMID: 20947508] (WB, Human) WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HRD1 Antibody (NB100-2526). (Showing 1 - 1 of 1 FAQs).

  1. What is the molecular weight for HRD1?
    • The theoretical molecular weight for HRD1 is 67kDa.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on HRD1.

OS9: Taking proteins to the ER finish line
The OS9 protein is a lectin/glycoprotein that maintains endoplasmic reticulum (ER) quality control and ER-associated degradation (the so-called ERAD pathway) of newly synthesized proteins. It is essential for the recognition of terminally misfolded no...  Read full blog post.

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Bioinformatics

Gene Symbol SYVN1
Entrez
Uniprot