Western Blot: HRD1 Antibody [NB100-2526] - Detection of HRD1 in an endogenous 293T cell lysate.
Immunocytochemistry/ Immunofluorescence: HRD1 Antibody [NB100-2526] - HRD1 antibody was tested in Hek293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: HRD1 Antibody [NB100-2526] - FFPE tissue section of human tonsil using rabbit polyclonal HRD1 antibody at 2 ug/mL concentration (1:500 dilution). The primary antibody binding to SYVN1/HRD1 ...read more
Heat samples at 50C for 30 minutes to detect HRD1 by Western blot. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24453213). KO Validation was reported in scientific literature (PMID: 26551274).
Hamster reactivity reported in scientific literature (PMID: 25078082).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Alternate Names for HRD1 Antibody
E3 ubiquitin-protein ligase synoviolin
KIAA1810Synovial apoptosis inhibitor 1
synovial apoptosis inhibitor 1, synoviolin
HRD1 (synovial apoptosis inhibitor 1 or SYVN1) is a ubiquitin ligase whose expression is induced by UPR following ER stress and it provides protection to cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in ER. HRD1 acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from ER-associated UBC7 E2 ligase and transfers it to target proteins for promoting their degradation. It is a component of ER's quality control system called ERAD (ER-associated degradation) which is involved in ubiquitin-dependent degradation of misfolded ER proteins. HRD1 also facilitates the degradation of normal/naturally short-lived proteins such as SGK, and has been shown to protect neuronal cells from apoptosis triggered by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by prooting their degradation. Moreover, HRD1 sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
OS9: Taking proteins to the ER finish line The OS9 protein is a lectin/glycoprotein that maintains endoplasmic reticulum (ER) quality control and ER-associated degradation (the so-called ERAD pathway) of newly synthesized proteins. It is essential for the recognition of terminally misfolded no... Read full blog post.
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