HRD1 Antibody

Images

 
Western Blot: HRD1 Antibody [NB100-2526] - Hrd1 is required for CDT intoxication. Co-immunoprecipitation of Derl2 and Hrd1. Derl2 was immunoprecipitated and samples were analyzed for Hrd1 by western blot. Image ...read more
Immunocytochemistry/ Immunofluorescence: HRD1 Antibody [NB100-2526] - HRD1 antibody was tested in Hek293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: HRD1 Antibody [NB100-2526] - FFPE tissue section of human tonsil using rabbit polyclonal HRD1 antibody at 2 ug/mL concentration (1:500 dilution). The primary antibody binding to SYVN1/HRD1 ...read more
Western Blot: HRD1 Antibody [NB100-2526] - Total protein from human HeLa, Hek293 and mouse 3T3 cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more

Product Details

Summary
Reactivity Hu, Mu, HaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HRD1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of human HRD1 (within residues 300-400). [UniProt Q96PK3]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYVN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockout Validated
Application Notes
Heat samples at 50C for 30 minutes to detect HRD1 by Western blot. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24453213). KO Validation was reported in scientific literature (PMID: 26551274).
Control
HRD1 Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-2526 in the following applications:

Publications
Read Publications using
NB100-2526 in the following applications:

  • 2 publications
  • WB
    8 publications

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 25078082).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HRD1 Antibody

  • E3 ubiquitin-protein ligase synoviolin
  • EC 6.3.2
  • HRD1EC 6.3.2.-
  • KIAA1810Synovial apoptosis inhibitor 1
  • MGC40372
  • synovial apoptosis inhibitor 1, synoviolin

Background

HRD1 (synovial apoptosis inhibitor 1 or SYVN1) is a ubiquitin ligase whose expression is induced by UPR following ER stress and it provides protection to cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in ER. HRD1 acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from ER-associated UBC7 E2 ligase and transfers it to target proteins for promoting their degradation. It is a component of ER's quality control system called ERAD (ER-associated degradation) which is involved in ubiquitin-dependent degradation of misfolded ER proteins. HRD1 also facilitates the degradation of normal/naturally short-lived proteins such as SGK, and has been shown to protect neuronal cells from apoptosis triggered by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by prooting their degradation. Moreover, HRD1 sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HRD1 Antibody (NB100-2526)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(2)
WB
(8)
All Applications
Filter By Species
Human
(4)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-2526 Applications Species
Jiang LL, Zhu B, Zhao Y et al. Membralin deficiency dysregulates astrocytic glutamate homeostasis leading to ALS-like impairment J. Clin. Invest. May 21 2019 [PMID: 31112137] (IHC-P, Human, Mouse) IHC-P Human, Mouse
Xie H, Tang CA, Song JH, Mancuso A. IRE1a RNase-dependent lipid homeostasis promotes survival in Myc-transformed cancers. J. Clin. Invest. 2018 Feb 26 [PMID: 29381485] (WB, Human) WB Human
Hwang J, Walczak CP, Shaler TA et al. Characterization of protein complexes of the endoplasmic reticulum associated degradation E3 ubiquitin ligase Hrd1. J. Biol. Chem. Apr 14 2017 [PMID: 28411238] (WB, Human) WB Human
Koenig Pa, Nicholls Pk, Schmidt Fi et al. The E2 Ubiquitin Conjugating Enzyme UBE2J1 is Required for Spermiogenesis in Mice. J. Biol. Chem. 2014 Oct 15 [PMID: 25320092] (WB, Mouse) WB Mouse
Eshraghi A, Dixon SD, Tamilselvam B et al. Cytolethal Distending Toxins Require Components of the ER-Associated Degradation Pathway for Host Cell Entry PLoS Pathog. 2014 Jul 01 [PMID: 25078082]
Sun S, Shi G, Han X et al. Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival. Proc. Natl. Acad. Sci. U.S.A. 2014 Feb 5 [PMID: 24453213] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Francisco AB, Singh R, Sha H et al. Haploid insufficiency of suppressor enhancer Lin12 1 like (SEL1L) predisposes mice to high fat diet-induced hyperglycemia. J Biol Chem;286(25):22275-82. 2011 Jun 24. [PMID: 21536682]
Dougan SK, Hu CC, Paquet ME et al. Derlin-2-deficient mice reveal an essential role for protein dislocation in chondrocytes Mol Cell Biol 2011 Mar [PMID: 21220515] (WB, Mouse) WB Mouse
Soundararajan R, Wang J, Melters D, Pearce D. Glucocorticoid-induced Leucine zipper 1 stimulates the epithelial sodium channel by regulating serum- and glucocorticoid-induced kinase 1 stability and subcellular localization. J Biol Chem 2010 Dec 17 [PMID: 20947508] (WB, Human) WB Human
Sun S, Shi G, Sha H et al. IRE1 [alpha] is an endogenous substrate of endoplasmic-reticulum-associated degradation. Nature cell Biology. 2015 Nov 09 (WB, Mouse) WB Mouse
Show All 11 Publications.

Reviews for HRD1 Antibody (NB100-2526) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB100-2526:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot HRD1 NB100-2526
Enlarge
5
reviewed by:
Anonymous
WB Human 04/04/2020
View

Summary

ApplicationWestern Blot
Sample TestedRKO,AR42J human acinar pancreatic cancer cell line
SpeciesHuman
LotA-2
Western Blot HRD1 NB100-2526
Enlarge
5
reviewed by:
Anonymous
WB Human 11/06/2018
View

Summary

ApplicationWestern Blot
Sample Tested293T,MDA MB 231 cells
SpeciesHuman
LotA-2
Western Blot HRD1 NB100-2526
Enlarge
5
reviewed by:
Anonymous
WB Human 10/17/2018
View

Summary

ApplicationWestern Blot
Sample Tested293T and HePG2 cell lysate
SpeciesHuman
LotA-2

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HRD1 Antibody (NB100-2526). (Showing 1 - 1 of 1 FAQs).

  1. What is the molecular weight for HRD1?
    • The theoretical molecular weight for HRD1 is 67kDa.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for HRD1 Antibody (NB100-2526)

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Research Areas for HRD1 Antibody (NB100-2526)

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Blogs on HRD1.

OS9: Taking proteins to the ER finish line
The OS9 protein is a lectin/glycoprotein that maintains endoplasmic reticulum (ER) quality control and ER-associated degradation (the so-called ERAD pathway) of newly synthesized proteins. It is essential for the recognition of terminally misfolded no...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Anonymous
04/04/2020
Application: WB
Species: Human

Anonymous
11/06/2018
Application: WB
Species: Human

Anonymous
10/17/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol SYVN1
Entrez
Uniprot