| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2526SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89667-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89668-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02998-20ul | |||
![Western Blot: HRD1 Antibody [NB100-2526] - Hrd1 is required for CDT intoxication. Co-immunoprecipitation of Derl2 and Hrd1. Derl2 was immunoprecipitated and samples were analyzed for Hrd1 by western blot. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004295) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HRD1-Antibody-Western-Blot-NB100-2526-img0006.jpg "Western Blot: HRD1 Antibody [NB100-2526] - Hrd1 is required for CDT intoxication. Co-immunoprecipitation of Derl2 and Hrd1. Derl2 was immunoprecipitated and samples were analyzed for Hrd1 by western blot. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004295) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: HRD1 Antibody [NB100-2526] - HRD1 antibody was tested in Hek293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2526-img0003.jpg)
![Immunohistochemistry-Paraffin: HRD1 Antibody [NB100-2526] - FFPE tissue section of human tonsil using rabbit polyclonal HRD1 antibody at 2 ug/mL concentration (1:500 dilution). The primary antibody binding to SYVN1/HRD1 antigen in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This HRD1 antibody generated an expected cytoplasmic staining of HRD1 with stronger signal around periphery of the nucleus signifying the staining of the endoplasmic reticulum.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NB100-2526-img0004.jpg)
![Western Blot: HRD1 Antibody [NB100-2526] - Total protein from human HeLa, Hek293 and mouse 3T3 cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HRD1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/HRD1-Antibody-Western-Blot-NB100-2526-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HRD1 Antibody [NBP1-89667] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & endoplasmic reticulum.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89667-img0008.jpg "Immunocytochemistry/Immunofluorescence: HRD1 Antibody [NBP1-89667] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & endoplasmic reticulum.")
![Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human lymph node.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89667-img0014.jpg)
![Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89667-img0002.jpg)
![Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89667-img0009.jpg)
![Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89667-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining in human tonsil and skeletal muscle tissues using anti-SYVN1 antibody. Corresponding SYVN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89667-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human cerebral cortex, lymph node, skeletal muscle and tonsil using Anti-SYVN1 antibody NBP1-89667 (A) shows similar protein distribution across tissues to independent antibody NBP1-89668 (B).](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89667-img0012.jpg)
![Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89667-img0013.jpg)
![Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89668] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89668-img0009.jpg "Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89668] - Staining of human cerebral cortex.")
![Immunohistochemistry: HRD1 Antibody [NBP1-89668] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-NBP1-89668-img0003.jpg)
![Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89668] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89668-img0004.jpg)
![Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89668] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89668-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89668] - Staining in human tonsil and skeletal muscle tissues using anti-SYVN1 antibody. Corresponding SYVN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89668-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89668] - Staining of human cerebral cortex, lymph node, skeletal muscle and tonsil using Anti-SYVN1 antibody NBP1-89668 (A) shows similar protein distribution across tissues to independent antibody NBP1-89667 (B).](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89668-img0007.jpg)
![Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89668] - Staining of human lymph node.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89668-img0008.jpg)
![Western Blot: HRD1 Antibody (JE44-67) [NBP3-32991] - Western blot analysis of HRD1 on different lysates with Rabbit anti-HRD1 antibody (NBP3-32991) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: Raji cell lysate Lane 4: HCT 116 cell lysate Lane 5: HeLa cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32991) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32991_rabbit-hrd1-mab-je44-67-288202412493459.jpg "Western Blot: HRD1 Antibody (JE44-67) [NBP3-32991] - Western blot analysis of HRD1 on different lysates with Rabbit anti-HRD1 antibody (NBP3-32991) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: Raji cell lysate Lane 4: HCT 116 cell lysate Lane 5: HeLa cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32991) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: HRD1 Antibody (JE44-67) [NBP3-32991] - Immunocytochemistry analysis of HeLa cells labeling HRD1 with Rabbit anti-HRD1 antibody (NBP3-32991) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HRD1 antibody (NBP3-32991) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32991_rabbit-hrd1-mab-je44-67-29202410484812.jpg)
![Flow Cytometry: HRD1 Antibody (JE44-67) [NBP3-32991] - Flow cytometric analysis of HeLa cells labeling HRD1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32991, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32991_rabbit-hrd1-mab-je44-67-29202416453233.jpg)
![Western Blot: HRD1 Antibody - BSA Free [HRD1] - Western blot analysis of lysates from Mouse pancreas using HRD1 Rabbit pAb at 1:3000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-02998_rabbit-polyclonal-hrd1-antibody-52202516321432.jpg "Western Blot: HRD1 Antibody - BSA Free [HRD1] - Western blot analysis of lysates from Mouse pancreas using HRD1 Rabbit pAb at 1:3000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.")
![Western Blot: HRD1 Antibody - BSA Free [HRD1] - Western blot analysis of various lysates using HRD1 Rabbit pAb at 1:3000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-02998_rabbit-polyclonal-hrd1-antibody-5220251640286.jpg)
![Western Blot: HRD1 Antibody (4H4) [H00084447-M01] - Analysis of SYVN1 expression in transfected 293T cell line by SYVN1 monoclonal antibody (M01), clone 4H4.Lane 1: SYVN1 transfected lysate(67.685 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HRD1-Antibody-4H4-Western-Blot-H00084447-M01-img0002.jpg "Western Blot: HRD1 Antibody (4H4) [H00084447-M01] - Analysis of SYVN1 expression in transfected 293T cell line by SYVN1 monoclonal antibody (M01), clone 4H4.Lane 1: SYVN1 transfected lysate(67.685 KDa).Lane 2: Non-transfected lysate.")
![ELISA: HRD1 Antibody (4H4) [H00084447-M01] - Detection limit for recombinant GST tagged SYVN1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HRD1-Antibody-4H4-Sandwich-ELISA-H00084447-M01-img0001.jpg)
![Immunohistochemistry-Paraffin: HRD1 Antibody [NBP3-06671] - HRD1 Antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NBP3-06671-img0001.jpg "Immunohistochemistry-Paraffin: HRD1 Antibody [NBP3-06671] - HRD1 Antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Western Blot: HRD1 Overexpression Lysate (Adult Normal) [NBL1-16659] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HRD1.](http://images.novusbio.com/fullsize/HRD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16659-img0002.jpg "Western Blot: HRD1 Overexpression Lysate (Adult Normal) [NBL1-16659] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HRD1.")
![Western Blot: HRD1 Overexpression Lysate (Adult Normal) [NBL1-16660] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HRD1.](http://images.novusbio.com/fullsize/HRD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16660-img0002.jpg "Western Blot: HRD1 Overexpression Lysate (Adult Normal) [NBL1-16660] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HRD1.")