![Boiled and unboiled HepG2 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR/gp78 antibody [HL2865] (NBP3-48740) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](http://images.novusbio.com/fullsize/nbp3-48740_rabbit-amfr-gp78-mab-hl2865-western-blot-1411202518391012.jpg "Boiled and unboiled HepG2 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR/gp78 antibody [HL2865] (NBP3-48740) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | HL2865 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit AMFR/gp78 Antibody (HL2865) (NBP3-48740) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of Human AMFR/gp78. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | AMFR |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for AMFR/gp78 Antibody (NBP3-48740)Find related products by research area.
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| Gene Symbol | AMFR |
![AMFR/gp78 antibody [HL2865] detects AMFR/gp78 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.AMFR/gp78 stained by AMFR/gp78 antibody [HL2865] (NBP3-48740) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 20 min](http://images.novusbio.com/fullsize/nbp3-48740_rabbit-amfr-gp78-mab-hl2865-immunohistochemistry-paraffin-141120251838419.jpg "AMFR/gp78 antibody [HL2865] detects AMFR/gp78 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.AMFR/gp78 stained by AMFR/gp78 antibody [HL2865] (NBP3-48740) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 20 min")
![Boiled and unboiled mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR/gp78 antibody [HL2865] (NBP3-48740) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48740_rabbit-amfr-gp78-mab-hl2865-western-blot-1411202518252016.jpg "Boiled and unboiled mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR/gp78 antibody [HL2865] (NBP3-48740) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Boiled and unboiled rat tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR/gp78 antibody [HL2865] (NBP3-48740) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48740_rabbit-amfr-gp78-mab-hl2865-western-blot-1411202518373814.jpg "Boiled and unboiled rat tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR/gp78 antibody [HL2865] (NBP3-48740) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")